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Purified anti-human CD274 (B7-H1, PD-L1) Antibody

Pricing & Availability
Clone
29E.2A3 (See other available formats)
Regulatory Status
RUO
Other Names
Programmed cell death ligand 1 (PD-L1), B7 homolog 1 (B7-H1)
Isotype
Mouse IgG2b, κ
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Product Citations
publications
29E.2A3_Purified_CD274_Antibody_FC_091013
PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with purified CD274 (clone 29E.2A3) (filled histogram) or purified mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • 29E.2A3_Purified_CD274_Antibody_FC_091013
    PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with purified CD274 (clone 29E.2A3) (filled histogram) or purified mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • 29E.2A3_Purified_CD274_Antibody_IHC-P_112316
    Human paraffin embedded tonsil tissue stained with purified anti-human CD274 (red) antibody. The nuclei were counterstained with DAPI (blue). See additional supplemental data for detailed information.
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329701 25 µg 48€
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329702 100 µg 67€
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Description

CD274, also known as PD-L1 and B7-H1, is type I transmembrane glycoprotein that serves as a ligand for CD279 (PD-1). This interaction is believed to regulate the balance between the stimulatory and inhibitory signals needed for responses to microbes and maintenance of self-tolerance. CD274 is involved in the costimulation of T cell proliferation and IL-10 and IFN-γ production in an IL-2-dependent and CD279-independent manner. Conflicting data has shown that CD274 can inhibit T cell proliferation and cytokine production, and alternatively, enhance T cell activation. Other studies suggest that CD274 may signal bidirectionally, raising interesting implications for its expression in a wide variety of cell types, including T and B cells, antigen-presenting cells, and nonhematopoietic cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human PD-L1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region5. Additional reported applications (for the relevant formats) include: blocking1-3 and immunohistochemical staining of acetone-fixed frozen sections1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329715, 329716, 329745 - 329748). 

It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor® 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the Alexa Fluor® 700 conjugate of interest.

Clone 29E.2A3 does not work in Western blot applications7.

Application References
  1. Brown J, et al. 2003. J. Immunol. 170:1257. (FC, IHC, Block)
  2. Radziewicz H, et al. 2007. J. Virol. 81:2545. (Block)
  3. Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (Block)
  4. Barsoum IB, et al. 2014. Cancer Res. 74:665. PubMed
  5. Haile, S et al. 2013. J. Immunol. 191:2829.
  6. RL M, et al. 2015. PNAS. 112:6506-6514. PubMed
  7. Mahoney KM, et al. 2015. Cancer Immunol. Res. 3:1308.
Product Citations
  1. Lee SWL, et al. 2018. Front Immunol. 8:1064. PubMed
  2. Wang Y, et al. 2019. Sci Rep. 9:566. PubMed
  3. Salvany‐Celades M et al. 2019. Cell Rep. 27(9):2537-2547 . PubMed
  4. Baruah P, et al. 2019. Front Immunol. 10:1644. PubMed
  5. Acheampong E, et al. 2019. Cancers (Basel). 11. PubMed
  6. Hartley GP, et al. 2018. Cancer Immunol Res. 6:1260. PubMed
  7. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  8. Juttukonda LJ, et al. 2017. Cell Host Microbe. 22:531. PubMed
  9. Nolan E, et al. 2017. Sci Transl Med. 9:eaal4925. PubMed
  10. Horn LA, et al. 2017. Oncotarget. 8:57964. PubMed
  11. Kim MH, et al. 2018. Cancer Immunol Res. 0.427083333. PubMed
  12. Hsu DC, et al. 2018. Clin Infect Dis. 67:437. PubMed
  13. Agrawal N, et al. 2018. Front Immunol. 2.053472222. PubMed
  14. Komohara Y, et al. 2018. J Clin Exp Hematop. 58:152. PubMed
  15. Kim DH, et al. 2019. Exp Mol Med. 51:94. PubMed
  16. Burr ML, et al. 2017. Nature. 549:101. PubMed
  17. Enyindah-Asonye G, et al. 2019. Front Immunol. 10:511. PubMed
  18. Zhou B, et al. 2020. Theranostics. 10:6530. PubMed
  19. Janse van Rensburg HJ, et al. 2018. Cancer Res. 78:1457. PubMed
  20. Parackova Z, et al. 2020. Sci Rep. 0.759027778. PubMed
  21. Barsoum I, et al. 2014. Cancer Res. 74:665. PubMed
  22. Hamanishi J, et al. 2015. J Clin Oncol. 101200/JCO2015623397. PubMed
  23. Li C, et al. 2016. Nat Commun. 7:12632. PubMed
  24. Hang Q, et al. 2020. Zhongguo Fei Ai Za Zhi. 1.539583333. PubMed
  25. Schwabenland M, et al. 2021. Immunity. . PubMed
  26. Zheng G, et al. 2021. Signal Transduct Target Ther. 6:236. PubMed
  27. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
  28. Garcia de Moura R, et al. 2021. Front Immunol. 12:632667. PubMed
  29. Zeller AN, et al. 2021. Eur Cell Mater. 41:603. PubMed
  30. Saber MM, et al. 2022. Antibodies (Basel). 11:. PubMed
  31. Heesters BA, et al. 2021. J Exp Med. 218:. PubMed
  32. Bouchard G, et al. 2022. Cancer Res. 82:648. PubMed
  33. Paterson K, et al. 2022. IEEE Open J Eng Med Biol. 3:86. PubMed
  34. Perdigoto AL, et al. 2022. JCI Insight. 7:. PubMed
  35. Hwang HJ, et al. 2021. Immun Inflamm Dis. 9:274. PubMed
  36. Yonemitsu K, et al. 2022. Sci Rep. 12:12007. PubMed
  37. Alpert A, et al. 2022. Cell Syst. 13:71. PubMed
  38. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  39. Qiao J, et al. 2022. PLoS One. 17:e0277956. PubMed
  40. Niu Q, et al. 2022. Proc Natl Acad Sci U S A. 119:e2213236119. PubMed
  41. Zhang G, et al. 2023. J Immunother Cancer. 11:. PubMed
RRID
AB_940370 (BioLegend Cat. No. 329701)
AB_940372 (BioLegend Cat. No. 329702)

Antigen Details

Distribution

T cells, B cells, NK cells, monocytes/macrophages, granulocytes and dendritic cells

Function
CD274 is involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-CD1-independent manner. Its interaction with PD-1-CD1 inhibits T-cell proliferation and cytokine production.
Ligand/Receptor
PD-1 (PDCD1)
Cell Type
B cells, Dendritic cells, Fibroblasts, Granulocytes, Macrophages, Monocytes, NK cells, T cells
Biology Area
Cancer Biomarkers, Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.

Gene ID
29126 View all products for this Gene ID
UniProt
View information about CD274 on UniProt.org

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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