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- Clone
- MD-1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 507501 | 50 µg | 85€ | ||||
| 507502 | 500 µg | 226€ | ||||
Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The 4S.B3 antibody reacts with the human IFN-γ. The MD-1 antibody reacts with human and monkey interferon-gamma (IFN-γ). The MD-1 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
ICFC, IHC-F, WB - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, the antibody should be titrated between 0.25 - 2 µg/ml to determine optimal condition. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA1,2,5 and ELISPOT3,4 Capture: The Purified MD-1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4S.B3 (Cat. No. 502504/502514) antibody as the detecting antibody and recombinant human IFN-γ (Cat. No. 570209) as the standard. The LEAF™ Purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 0.5-2 µg/ml is recommended.
Neutralization1: The LEAF™ Purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IFN-γ bioactivity (Cat. No. 507513).
Additional reported applications (for the relevant formats) include: Western blotting1,2, intracellular staining for flow cytometry6, and immunohistochemical staining7 of paraformaldehyde-fixed, saponin-treated frozen tissue sections.
Note: For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended. - Application References
-
- Van der Meide P, et al. 1985. J. Immuno. Meth. 79:293.
- Yazdanbakhsh M, et al. 1993. Eur. J. Immuno. 23:3312.
- Besouw N, et al. 1994. J. Med. Primatol. 23:42.
- Van der Meide P, et al. 1995. J. Med. Primatol. 24:271.
- Hilkens C, et al. 1996. J. Immunol. 156:1722.
- Hamann D, et al. 1996. J. Immunol. 156:1387.
- Wassenaar A, et al. 1995. Infect. Immunity. 63:2147.
- Product Citations
- RRID
-
AB_2122340 (BioLegend Cat. No. 507501)
AB_2122339 (BioLegend Cat. No. 507502)
Antigen Details
- Structure
- Cytokine; dimer; 20-25 kD (Mammalian)
- Bioactivity
- Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
- Cell Sources
- CD8+ and CD4+ T cells, NK cells
- Cell Targets
- T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
- Receptors
- IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
- Cell Type
- Tregs
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151. - Regulation
- Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
- Gene ID
- 3458 View all products for this Gene ID
- UniProt
- View information about IFN-gamma on UniProt.org
Related Pages & Pathways
Pathways
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