- Clone
- J099B12 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Histidine Tag
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![J099B12_HRP_His-Tag_Antibody_1_061824 J099B12_HRP_His-Tag_Antibody_1_061824](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/J099B12_HRP_His-Tag_Antibody_1_061824.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg total protein) from HEK293 cells either non-transfected or transfected with an expression vector for a His-tagged protein were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and then probed with HRP anti-His Tag (clone J099B12). Direct-Blot ™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426318) was used as a detection agent. Lane M: Molecular weight marker -
ELISA assay was performed by coating a 96-well plate with 100 µL/well of a His-tagged recombinant protein (100 ng/mL). The coated His-tagged recombinant protein was detected with HRP anti-His Tag Antibody (Clone J099B12) followed by TMB High Sensitivity Substrate Solution (Cat. No. 421501). Absorbance was measured at 450 nm.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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652503 | 25 µg | 90€ | ||||
652504 | 100 µg | 329€ |
This antibody was raised against linear 8XHis tag. It reacts with His tag protein, either C, N, or internal His6 and up repeats. It can be used for Western blotting to detect linear His repeat (His6 or more).
Product DetailsProduct Details
- Verified Reactivity
- Epitope tag
- Reported Reactivity
- Species independent
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH-conjugated His-tag peptide
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solution with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light. Avoid exposure to sodium azide.
- Application
-
WB - Quality tested
ELISA - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per mL. For direct ELISA, a concentration range of 0.001 - 0.1 µg per mL is recommended. Avoid using diluent solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the end-user titrate each lot of reagents for optimal performance.
- Application References
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- Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
- Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
- Product Citations
-
- RRID
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AB_2734520 (BioLegend Cat. No. 652503)
AB_2563555 (BioLegend Cat. No. 652504)
Antigen Details
- Function
- Recombinant poly-histidine tag detection
- Biology Area
- Cell Biology
- Gene ID
- NA
- UniProt
- View information about His Tag on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All His Tag Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-His Tag | J099B12 | WB,ICC |
HRP anti-His Tag | J099B12 | WB,ELISA |
Ultra-LEAF™ Purified anti-His Tag | J099B12 | WB |
Alexa Fluor® 488 anti-His Tag | J099B12 | ICC |
Alexa Fluor® 594 anti-His Tag | J099B12 | ICC |
Alexa Fluor® 647 anti-His Tag | J099B12 | ICC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-His Tag
Western blot analysis of cell lysates from HeLa cells transf... Immunofluorescence of HeLa cells transfected without (A) or ... Insect cell supernatant expressing His-tagged mouse CCL22 wa... -
HRP anti-His Tag
Whole cell extracts (15 µg total protein) from HEK293 cells ... ELISA assay was performed by coating a 96-well plate with 10... -
Ultra-LEAF™ Purified anti-His Tag
0.1µl recombinant Posi-Tag Epitope Tag Protein was resolved ... -
Alexa Fluor® 488 anti-His Tag
Hela cells transfected with multi-tag plasmid were fixed wi... -
Alexa Fluor® 594 anti-His Tag
Hela cells transfected with multi-tag plasmid were fixed wit... -
Alexa Fluor® 647 anti-His Tag
Hela cells transfected with multi-tag plasmid were fixed wit...
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