Loading...

PE anti-mouse CD80 Antibody

Pricing & Availability
Clone
16-10A1 (See other available formats)
Regulatory Status
RUO
Other Names
B7-1, B7, Ly-53
Isotype
Armenian Hamster IgG
Ave. Rating
Submit a Review
Product Citations
publications
16-10A1_PE_032309.jpg
Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with 16-10A1 PE
  • 16-10A1_PE_032309.jpg
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with 16-10A1 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
104707 50 µg 88€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
104708 200 µg 235€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Reported Reactivity
Dog
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
CHO cell line transfected with mouse B7 (CD80)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104747-104752).

Application References
  1. Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC)
  2. Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  5. Ma XT, et al. 2006. Cancer Res. 66:1169.
  6. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC)
  7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  8. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  9. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed
  10. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  11. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
Product Citations
  1. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  2. Sohn HS, et al. 2022. Sci Adv. 8:eabo5284. PubMed
  3. Chen H, et al. 2023. Nat Commun. 14:941. PubMed
  4. Cheng K, et al. 2023. Small. 19:e2300125. PubMed
  5. Sang H, et al. 2023. Adv Clin Exp Med. 32:551. PubMed
  6. Ma X, et al. 2023. Nat Commun. 14:1606. PubMed
  7. Huang T, et al. 2023. Front Immunol. 14:1124152. PubMed
  8. Fu S, et al. 2023. Nat Commun. 14:2248. PubMed
  9. Li T, et al. 2023. Nat Commun. 14:2498. PubMed
  10. Li Y, et al. 2023. Front Cell Infect Microbiol. 13:1117230. PubMed
  11. Wu N, et al. 2023. Front Bioeng Biotechnol. 11:1173381. PubMed
  12. Wang K, et al. 2023. Nat Commun. 14:2950. PubMed
  13. Wang X, et al. 2023. Stem Cell Rev Rep. . PubMed
  14. Lu R, et al. 2022. Scand J Immunol. 96:e13177. PubMed
  15. Wang S, et al. 2022. Sci Adv. 8:eabn3883. PubMed
  16. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  17. Blanco LP, et al. 2022. Arthritis Rheumatol. 74:1971. PubMed
  18. Sun Y, et al. 2023. Bioact Mater. 22:47. PubMed
  19. Liu M, et al. 2022. Cells. 11: . PubMed
  20. Montilla A, et al. 2023. Cell Death Dis. 14:16. PubMed
  21. Lombard-Vadnais F, et al. 2023. iScience. 26:105852. PubMed
  22. Han J, et al. 2023. Front Microbiol. 13:1067725. PubMed
  23. Rozanski C, et al. 2011. J Exp Med. 208:1435. PubMed
  24. Mulder R, et al. 2017. Front Immunol. 1.464583333. PubMed
  25. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  26. Wilhelm J, et al. 2021. J Control Release. 329:353. PubMed
  27. Takeda Y, et al. 2018. Innate Immun. 24:323. PubMed
  28. Kashyap AS, et al. 2019. Cell Rep. 28:3367. PubMed
  29. Wei H, et al. 2021. Malar J. 20:89. PubMed
  30. Gruber T, et al. 2020. JCI Insight. 5:00. PubMed
  31. Kodumudi KN, et al. 2019. Front Immunol. 10:1939. PubMed
  32. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  33. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  34. Kremenovic M, et al. 2022. J Immunother Cancer. 10:. PubMed
  35. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
  36. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  37. Rafiq S, et al. 2018. Nat Biotechnol. 36:847. PubMed
  38. Liang Z, et al. 2021. J Cell Physiol. 236:4725. PubMed
  39. Mardomi A, et al. 2021. J Cell Physiol. 236:6328. PubMed
  40. Jiang S, et al. 2020. Scand J Immunol. e12867:91. PubMed
  41. Sharma S, et al. 2015. J Immunol. 194:5529. PubMed
  42. Bhattacharjee P, et al. 2018. Sci Rep. 13:e0199785. PubMed
  43. Wu J, et al. 2021. Mol Med Rep. 23:. PubMed
  44. Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
  45. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  46. Lin SY, et al. 2017. Autophagy. 14:778. PubMed
  47. Utley A, et al. 2020. Cell Rep. 31:107815. PubMed
  48. Tsukasaki M, et al. 2020. Cell Reports. 32(10):108124. PubMed
  49. Zhang L, et al. 2021. J Immunother Cancer. 9:. PubMed
  50. Xie Z, et al. 2020. Light Sci Appl. 0.486805556. PubMed
  51. Zhang F, et al. 2017. Mol Immunol. 10.1016/j.molimm.2017.06.019. PubMed
  52. Pardy RD, et al. 2021. Nat Commun. 12:4051. PubMed
  53. Coe D, et al. 2022. JCI Insight. 7:. PubMed
  54. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  55. Mulas F, et al. 2020. Cell Mol Immunol. . PubMed
  56. Xie Z, et al. 2018. J Am Heart Assoc. 7:e007442. PubMed
  57. Florêncio KGD, et al. 2022. Front Immunol. 13:941757. PubMed
  58. Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed
  59. Guo L, et al. 2021. Vaccines (Basel). 9:. PubMed
  60. Ganesan R, et al. 2020. FASEB J. 34:15888. PubMed
  61. Pilones KA, et al. 2020. Cancer Immunol Res. 8:1054. PubMed
  62. Chen G, et al. 2021. Sci Adv. 7:eabg5686. PubMed
  63. Wong E, et al. 2012. J Immunol. 189:5667. PubMed
  64. White C, et al. 2015. J Immunol. 194:697. PubMed
  65. Matundan H, et al. 2019. J Virol. 93. PubMed
  66. Matundan HH, et al. 2021. J Virol. 95:e0103621. PubMed
  67. Wu G, et al. 2022. Wellcome Open Res. 6:134. PubMed
  68. Zheng D, et al. 2022. Acta Pharm Sin B. 12:2740. PubMed
  69. Vogel A, et al. 2022. STAR Protoc. 3:101653. PubMed
  70. Sun L, et al. 2015. Sci Rep. 5: 14871. PubMed
  71. Marshall N, et al. 2012. Cancer Res. 72:581. PubMed
  72. Yang C, et al. 2019. Nanomedicine (Lond). 14:2423. PubMed
  73. Tomay F, et al. 2019. J Transl Med. 17:237. PubMed
  74. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  75. Zhang X, et al. 2022. JCI Insight. 7:. PubMed
  76. Fan Q, et al. 2021. Bioact Mater. 6:4014. PubMed
  77. Ye Y, et al. 2017. Sci Immunol. 2:17. PubMed
  78. Zhang W, et al. 2020. Nat Commun. 11:1187. PubMed
  79. Zhang Q, et al. 2021. Front Cell Dev Biol. 9:655552. PubMed
  80. Taddio MF, et al. 2021. Mol Imaging Biol. 23:196. PubMed
  81. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  82. Kelly SH, et al. 2021. ACS Biomater Sci Eng. 7:1876. PubMed
  83. Li J, et al. 2022. Nat Commun. 13:4032. PubMed
  84. Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
  85. He J, et al. 2020. Evid Based Complement Alternat Med. 2020:5637507. PubMed
  86. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  87. Li Z, et al. 2022. Nat Commun. 13:6321. PubMed
  88. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
  89. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  90. Qi J, et al. 2021. Nat Commun. 12:4755. PubMed
  91. Li J, et al. 2020. Development. :. PubMed
  92. Miura N, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.01.020. PubMed
  93. Feng S, et al. 2021. Exp Ther Med. 21:20. PubMed
  94. Simmons D, et al. 2012. J Immunol. 188:3116. PubMed
  95. Zhang Q, et al. 2022. Commun Biol. 5:544. PubMed
  96. Walker MT, et al. 2021. Front. Allergy. 2:. PubMed
  97. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  98. Tateishi R, et al. 2015. PLoS One. 10: e0141650. PubMed
  99. Kelly SH, et al. 2021. Adv Healthc Mater. 10:e2001614. PubMed
  100. Piñón-Zárate G, et al. 2022. Molecules. 27:. PubMed
  101. Vogel A, et al. 2022. Cell Rep. 38:110420. PubMed
  102. Yang Z, et al. 2021. Nat Commun. 12:4299. PubMed
  103. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  104. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  105. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  106. Kadir NA, et al. 2020. Pathogens. 9:00. PubMed
  107. Xiao Z, et al. 2022. Mater Today Bio. 15:100297. PubMed
  108. Zhang X, et al. 2021. Front Pharmacol. 12:706748. PubMed
  109. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  110. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  111. Lightman SM, et al. 2020. Cell Reports. 1.086805556. PubMed
  112. Chen R, et al. 2021. Front Oncol. 11:743050. PubMed
  113. Wang Y, et al. 2020. Vaccines (Basel). 8:. PubMed
  114. Jeisy-Scott V, et al. 2012. J Virol. 86:10988. PubMed
  115. Liu WL, et al. 2019. Nat Commun. 10:3199. PubMed
  116. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  117. Zhang Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  118. Lin YC, et al. 2020. Mol Med Rep. 22:4909. PubMed
RRID
AB_313128 (BioLegend Cat. No. 104707)
AB_313129 (BioLegend Cat. No. 104708)

Antigen Details

Structure
Ig superfamily, 60 kD
Distribution

Macrophages, activated B cells and T cells, dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28 (stimulatory), CD152(CTLA4) (inhibitory)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.

Gene ID
12519 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 02/18/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account