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APC Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin-Allophycocyanin, SAv-APC
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Product Citations
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APC_Streptavidin_Antibody_042123
Human peripheral blood lymphocytes were stained with APC streptavidin alone (open histogram) or Biotin anti-human CD3 (clone UCHT1) followed by (APC) Streptavidin (filled histogram).
  • APC_Streptavidin_Antibody_042123
    Human peripheral blood lymphocytes were stained with APC streptavidin alone (open histogram) or Biotin anti-human CD3 (clone UCHT1) followed by (APC) Streptavidin (filled histogram).
See APC spectral data
Cat # Size Price Save
405207 100 µg ¥26,180
Description

Streptavidin binds to biotin with high affinity. Streptavidin-APC is useful for detecting biotinylated antibodies. The excitation of APC by 600-635 nm laser light induces a fluorescence emission maximum of 660 nm.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-APC solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this Streptavidin-APC is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the APC conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.125 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Streptavidin-APC is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-APC is 320 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Kamimura D, et al. 2006. J. Immunol. 177:306.
  2. Andoniou CE, et al. 2005. Nature Immunology 6:1011.
  3. Chou WC,et al.2006.Blood108:3005. PubMed
  4. Malagolini N, et al. 2009. Glycobiology. 19:172. PubMed
  5. Kroeger KM, et al. 2009. J. Leukocyte Biol. PubMed
  6. Imada M, et al. 2009. Int. Immunol. 21:1151. PubMed
  7. Yi Y, et al. 2010 J. Biol Chem. 285:21233. PubMed
  8. King IL, et al. 2010. J. Immunol. 185:6138. PubMed
  9. Yokoyama T, et al. 2011. Int Immunol. PubMed
  10. Rusert P, et al. 2011. J. Exp Med. 208:1419. PubMed
  11. Bollig N, et al. 2012. PNAS. 109:8664. PubMed
  12. Nguyen V, et al. 2012. J. Immunol. 189:1081. PubMed
  13. Jager NA, et al. 2012. J Nucl Med. 53:1229. PubMed
  14. Liberman AC, et al. 2012. Mol Immunol. 50:220. PubMed
  15. Ogawa H, et al. 2012. Biochem Biophys Res Commun. 420:114. PubMed
  16. Ludin A, et al.2012. Nat Immunol. 13:1072. PubMed
  17. Sugiura D, et al. 2012. PLoS One. 7:e44770. PubMed
  18. Tani-Ichi S, et al. 2012. PNAS. 110:612. PubMed
  19. Jin L, et al. 2013. PNAS. 110:3907. PubMed
  20. Ota T, et al. 2013. J. Immunol. 191:3179. PubMed
  21. Fu X, et al. 2013. PNAS. 110:17982. PubMed
  22. Ryan SO, et al. 2014. Glycobiology. 24:362. PubMed
  23. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  24. Pilling D, et al. 2014. PLoS One. 9:93730. PubMed
  25. Matthews JA, et al. 2014. PLoS One. 9:97707. PubMed
  26. Owen KA, et al. 2014. PLoS Pathog. 10:1004159. PubMed
  27. Duek A, et al. 2014. Blood. 123:3943. PubMed
  28. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  29. Skogberg G, et al. 2014. J Immunol. 193:2187. PubMed
  30. Lundberg P, et al. 2014. J Exp Med. PubMed
  31. Martin RK, et al. 2014. PLoS One. 9:110609. PubMed
  32. Rai S, et al. 2014. PLoS One. 9:109441. PubMed
  33. Santoyo-Ramos P, et al. 2014. PLoS One. 9:112580. PubMed
  34. Jager NA, et al. 2014. J Nucl Med. 55:1945. PubMed
  35. Kong S, et al. 2014. J Immunol. 193:5515. PubMed
  36. Cabrera-Perez J, et al. 2015. J Immunol. 194:1609. PubMed
  37. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
  38. Kim Yu, et al. 2015. PLoS One. 10:120294. PubMed
  39. Zhang L, et al. 2015. J Immunol. 194:5243. PubMed
Product Citations
  1. Goel RR, et al. 2022. Cell. 185:1875. PubMed
  2. Sugimoto M, et al. 2022. DNA Res. 29: . PubMed
  3. Nutalai R, et al. 2022. Cell. 185:2116. PubMed
  4. Singh S, et al. 2022. iScience. 25:104549. PubMed
  5. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  6. Scharf L, et al. 2023. JCI Insight. 8: . PubMed
  7. Cao Y, et al. 2023. Nature. 614:521. PubMed
  8. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  9. Hernandez SPA, et al. 2023. Front Immunol. 13:1035344. PubMed
  10. Pušnik J, et al. 2023. Nat Commun. 14:572. PubMed
  11. Gon Y, et al. 2023. PLoS One. 18:e0279389. PubMed
  12. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
  13. Dickerson KM, et al. 2022. Blood Cancer Discov. 3:240. PubMed
  14. Lopez R, et al. 2022. Nat Biotechnol. 40:1360. PubMed
  15. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  16. Suzuki M, et al. 2015. J Immunol. 195: 1273-1281. PubMed
  17. Preece R, et al. 2020. Gene Ther. 451:27. PubMed
  18. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
  19. Perruzza L, et al. 2019. Sci Rep. 9:9315. PubMed
  20. Cohen–Khait R, et al. 2017. Structure. 25:1867. PubMed
  21. White Z, et al. 2022. Blood Cells Mol Dis. 97:102688. PubMed
  22. Chen HY, et al. 2022. Elife. 11:. PubMed
  23. Parry H, et al. 2022. Cell Rep Med. 3:100739. PubMed
  24. Vyasamneni R, et al. 2023. Cell Rep Methods. 3:100388. PubMed
  25. Nihei Y, et al. 2023. Sci Adv. 9:eadd6734. PubMed
  26. Koutsakos M, et al. 2023. Immunity. 56:879. PubMed
  27. García-Vega M, et al. 2023. iScience. 26:106562. PubMed
  28. Liu Y, et al. 2023. Nat Commun. 14:2179. PubMed
  29. Hie B, et al. 2023. Nat Biotechnol. . PubMed
  30. Li X, et al. 2023. Nat Commun. 14:2929. PubMed
  31. Lan Y, et al. 2023. iScience. 26:106817. PubMed
  32. Tarban N, et al. 2022. Cells. 11:. PubMed
  33. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
  34. Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed
  35. Georgiadis C, et al. 2021. Leukemia. . PubMed
  36. Malagolini N, et al. 2009. Glycobiology. 0.911111111. PubMed
  37. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  38. Schneider C, et al. 2018. Cell. 174:271. PubMed
  39. Proietti M, et al. 2019. Nat Commun. 10:250. PubMed
  40. Temming AR, et al. 2021. Front Immunol. 12:594773. PubMed
  41. Blake SJ, et al. 2021. Cell Rep Med. 2:100464. PubMed
  42. Sarkar R, et al. 2021. Bio Protoc. 11:e4255. PubMed
  43. Hou S, et al. 2021. Sci Immunol. 6:eabg9433. PubMed
  44. Ota T, et al. 2013. J Immunol. 191:3179. PubMed
  45. Santoyo-Ramos P, et al. 2014. PLoS One. 9:112580. PubMed
  46. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  47. Viborg N, et al. 2019. Oncoimmunology. 8:e1663107. PubMed
  48. Pazina T, et al. 2019. Cancer Immunol Res. 7:1633. PubMed
  49. da Mota JB, et al. 2020. J Leukoc Biol. 108:1239. PubMed
  50. Niewolik D, et al. 2022. Nucleic Acids Res. 50:2096. PubMed
  51. Io S, et al. 2021. STAR Protoc. 2:100921. PubMed
  52. Yamanaka S, et al. 2021. STAR Protocols. 2(1):100314. PubMed
  53. J L, et al. 2016. Sci Rep. 6:30904. PubMed
  54. Gaudreault V, et al. 2015. PLoS One. 10: e0140805. PubMed
  55. Jager N, et al. 2014. J Nucl Med. 55:1945. PubMed
  56. Ludin A, et al. 2012. Nat Immunol. 13:1072. PubMed
  57. Jager N, et al. 2012. J Nucl Med. 53:1222. PubMed
  58. Kimura T, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 317:L271. PubMed
  59. Biffi G, et al. 2018. Cancer Discov. 2:282. PubMed
  60. Lebert-Ghali C, et al. 2017. Exp Hematol. 10.1016/j.exphem.2017.03.003. PubMed
  61. Mathews J, et al. 2014. PLoS One. 9:97707. PubMed
  62. Kim Y, et al. 2015. PLoS One. 10:120294. PubMed
  63. Jin L, et al. 2016. Stem Cell Res. 16:40-53. PubMed
  64. Palm A, et al. 2016. Sci Rep. 10.1038/srep20531. PubMed
  65. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  66. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  67. Catapano M, et al. 2020. J Invest Dermatol. 140:816. PubMed
  68. Rusert P, et al. 2011. J Exp Med. 208:1419. PubMed
  69. Liu C, et al. 2022. Cell Host Microbe. 30:53. PubMed
  70. She L, et al. 2021. JCI Insight. 6:e143509. PubMed
  71. Cao Y, et al. 2022. Nature. :. PubMed
  72. Sato Y, et al. 2021. J Clin Invest. Online ahead of print. PubMed
  73. Toulmin SA, et al. 2021. Nat Commun. 12:3993. PubMed
  74. Barcala Tabarrozzi A, et al. 2016. Sci Rep. 6:36646. PubMed
  75. Kong S, et al. 2014. J Immunol. 193:5515. PubMed
  76. Gui J, et al. 2020. Nat Cancer. 1:603. PubMed
  77. Duek A, et al. 2014. Blood. 123:3943. PubMed
  78. Yokoyama T, et al. 2011. J Immunol. 208:885. PubMed
  79. Cao Y, et al. 2022. Nature. 602:657. PubMed
  80. Kakizaki M, et al. 2021. Cell Death Dis. 12:1010. PubMed
  81. Calés C, et al. 2016. Mol Cell Biol. 36: 900 - 912. PubMed
  82. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
  83. Bollig N, et al. 2012. Proc Natl Acad Sci U S A. 109:8664. PubMed
  84. Yi Y, et al. 2010. J Biol Chem. 285:21233. PubMed
  85. Chou W, et al. 2006. Blood. 108:3005. PubMed
  86. Tashita C, et al. 2020. World J Gastroenterol. 26:918. PubMed
  87. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  88. Wang L, et al. 2022. Nat Commun. 13:4709. PubMed
  89. Kunitomi A, et al. 2022. Cell Rep Methods. 2:100317. PubMed
  90. Tani-ichi S, et al. 2012. Proc Natl Acad Sci U S A. 110:612. PubMed
  91. Ryan S, et al. 2014. Glycobiology. 24:262. PubMed
  92. Han X, et al. 2017. Mol Ther. 25(1):274-284. PubMed
  93. Hao Y, et al. 2020. J Biol Chem. . PubMed
  94. Parikh BA, et al. 2020. Cell Rep. 32:107969. PubMed
  95. Hansen UK, et al. 2020. Front Immunol. 11:373. PubMed
  96. Mizoguchi I, et al. 2020. J Clin Invest. 130:6124. PubMed
  97. Locke F, et al. 2017. Mol Ther. 25(1):285-295. PubMed
  98. Jennings R, et al. 2016. Int Immunol. 28: 503 - 512. PubMed
  99. Morita N, et al. 2016. Innate Immunity. 10.1177/1753425916672312. PubMed
  100. Rai S, et al. 2014. PLoS One. 9:109441. PubMed
  101. Owen K, et al. 2014. PLoS Pathog. 10:1004159. PubMed
  102. Sugiura D, et al. 2012. PLoS One. 7:e44770. PubMed
  103. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
  104. Friedrich SK, et al. 2021. Pathogens. 10: . PubMed
  105. Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed
  106. Nguyen V, et al. 2012. J Immunol. 189: 1081. PubMed
  107. Jin L, et al. 2013. Proc Natl Acad Sci U S A. 110:3907. PubMed
  108. James KR, et al. 2020. Nat Immunol. 1.113194444. PubMed
  109. Audemard-Verger A, et al. 2017. J Immunol. 10.4049/jimmunol.1700430. PubMed
  110. Hermann BP, et al. 2018. Cell Rep. 25:1650. PubMed
  111. Van Gool F, et al. 2019. Immunity. 50:362. PubMed
  112. Pascual G, et al. 2021. Nature. 599:485. PubMed
  113. Saini SK, et al. 2021. Sci Immunol. 6:. PubMed
  114. Mainini F, et al. 2015. PLoS One. 10: 0145403. PubMed
  115. Zhang L, et al. 2015. J Immunol. 194:5423. PubMed
  116. Skogberg G, et al. 2014. J Immunol. 193:2187. PubMed
  117. Ogawa H, et al. 2012. Biochem Biophys Res Commun. 420:114. PubMed
  118. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  119. Guichard V, et al. 2017. Elife. 6:e27215. PubMed
  120. Collins DR, et al. 2021. Immunity. 54:2372. PubMed
  121. Larcombe-Young D, et al. 2022. STAR Protoc. 3:101414. PubMed
  122. Pilling D, Gomer R 2014. PLoS One. 9:93730. PubMed
  123. Dejnirattisai W, et al. 2021. Cell. 2183:184. PubMed
  124. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  125. Jiang H, et al. 2011. PLoS One. 6:e27464. PubMed
  126. Tuekprakhon A, et al. 2022. Cell. 185:2422. PubMed
  127. Kim SP, et al. 2022. Cancer Immunol Res. :OF1. PubMed
  128. Robertson TF, et al. 2021. J Cell Biol. 220:. PubMed
  129. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  130. Lundberg P, et al. 2014. J Exp Med. 211:2213. PubMed
  131. King I, et al. 2010. J Immunol. 185:6138. PubMed
  132. Budai Z, et al. 2021. Cells. 10:. PubMed
  133. Bainter W, et al. 2021. Sci Immunol. 6:eabf6723. PubMed
  134. Martin R, et al. 2014. PLoS One. 9:110609. PubMed
  135. Gerdes P, et al. 2022. Nat Commun. 13:7470. PubMed
  136. Koide S, et al. 2022. iScience. 25:103603. PubMed
  137. Tian D, et al. 2019. Nat Commun. 10:4246. PubMed
  138. Yewdell WT, et al. 2021. Cell Rep. 37:109961. PubMed
  139. Nguyen NT, et al. 2021. Nat Nanotechnol. 16:1424. PubMed
  140. Kosti P, et al. 2021. STAR Protocols. 2:100723. PubMed
  141. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
  142. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
  143. Mazor Y, et al. 2017. Sci Rep. 7:40098. PubMed
  144. Wan X, Thomas J, Unanue E 2016. J Exp Med. 213: 967 - 978. PubMed
  145. Liberman A, et al. 2012. Mol Immunol. 50:220. PubMed
  146. GR N, et al. 2012. PLoS One. 7:e39045. PubMed
  147. Zahradník J, et al. 2021. ACS Synth Biol. Online ahead of print. PubMed
  148. Chen Y, et al. 2021. Cancer Res Treat. Online ahead of print. PubMed
  149. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  150. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  151. Tripathi S, et al. 2016. Proc Natl Acad Sci U S A. 113: 1555 - 1564. PubMed
  152. Hauser J, et al. 2016. Mol Immunol. 80:78-90. PubMed
  153. Wang W, et al. 2020. STAR Protoc. 1:100218. PubMed
  154. Pedersen NW, et al. 2019. Nat Commun. 10:837. PubMed
  155. Nagatake T, et al. 2019. Mucosal Immunol. 12:p1082. PubMed
  156. Yang C, et al. 2019. Nat Commun. 10:3931. PubMed
  157. Kroeger K, et al. 2009. J Leukoc Biol. 86:769. PubMed
  158. Fichtner ML, et al. 2022. Acta Neuropathol Commun. 10:154. PubMed
  159. Holm JS, et al. 2022. Nat Commun. 13:1935. PubMed
  160. Spanier J, et al. 2016. Nat Commun. 7:11804. PubMed
  161. Tagawa M, et al. 2016. PLoS One. 11: 0150030. PubMed
  162. Fu X, et al. 2013. Proc Natl Acad Sci U S A. 110:17892. PubMed

Antigen Details

Gene ID
NA
Specificity (DOES NOT SHOW ON TDS):
Biotin
Specificity Alt (DOES NOT SHOW ON TDS):
Biotin
App Abbreviation (DOES NOT SHOW ON TDS):
FC,ICFC
UniProt
View information about Biotin on UniProt.org

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Go To Top Version: 5    Revision Date: 04/21/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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