- Clone
- 429 (MVCAM.A) (See other available formats)
- Regulatory Status
- RUO
- Other Names
- VCAM-1, INCAM-110
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_429_Alx488_021808 1_429_Alx488_021808](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/429_Alx488_021808.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse bone marrow cells stained with 429 Alexa Fluor® 488 -
C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of CD106 (clone 429 (MVCAM.A)) Alexa Fluor® 488 (green) and CD117 (clone 2B8) Alexa Fluor® 594 (red) overnight at 4°C. The image was captured by 10X objective. -
Mice were injected subcutaneously with sheep red blood cells in a volume of 25 µL per site on days 0 and 4 and harvested on day 11. Confocal image of C57BL/6 mouse lymph node acquired using the IBEX method of highly multiplexed antibody-based imaging: CD206 (red) in Cycle 5 and CD106 (cyan) in Cycle 7. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH). -
Mice were injected subcutaneously with sheep red blood cells in a volume of 25 mL per site on days 0 and 4 and harvested on day 11. Confocal image of C57BL/6 mouse lymph node acquired using the IBEX method of highly multiplexed antibody-based imaging: CD206 (red) in Cycle 5 and CD106 (cyan) in Cycle 7. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse preadipose cell line PA6
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality testes
IHC-F - Verified
SB - Reported in the literature, not verified in house
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: immunohistochemical staining2,3,5-7 of acetone-fixed frozen sections, blocking4,5,8 of ligand binding in vitro and in vivo, immunoprecipitation1 , and spatial biology (IBEX)11,12. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105727 & 105728).
- Additional Product Notes
-
Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
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Application References
(PubMed link indicates BioLegend citation) -
- Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168. (IP)
- Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
- Ishiyama N, et al. 1998. Pathobiology 66:274. (IHC)
- Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
- Baron JL, et al. 1994. J. Clin. Invest. 93:1700. (Block IHC)
- Buck CA, et al. 1996. Cell Adhes. Commun. 4:69. (IHC)
- Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
- Meunier MC, et al. 2005. Nature Medicine 11:1222. (Block) PubMed
- Monnier J, et al. 2012. J. Immunol. 189:956. PubMed
- Motohashi N, et al. 2013. J Cell Sci. 126:2678. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
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- RRID
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AB_493427 (BioLegend Cat. No. 105710)
Antigen Details
- Structure
- Ig superfamily, 47 kD
- Distribution
-
Bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells
- Function
- Adhesion
- Ligand/Receptor
- VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin)
- Cell Type
- Dendritic cells, Endothelial cells, Mesenchymal Stem Cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168.
3. Bevilacquea MP. 1993. Annu. Rev. Immunol. 11:767.
4. Koni PA, et al. 2001. J. Exp. Med. 193:741. - Gene ID
- 22329 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- CD106
- Specificity Alt (DOES NOT SHOW ON TDS):
- CD106
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC,IHC-F,SB
- UniProt
- View information about CD106 on UniProt.org
Related FAQs
- If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?
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It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.
- Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?
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Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.
- Are other fluorophores compatible with IBEX?
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Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.
- The same antibody works in one tissue type but not another. What is happening?
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Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.
- How can I be sure the staining I’m seeing in my tissue is real?
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In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.
Other Formats
View All CD106 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Biotin anti-mouse CD106 | 429 (MVCAM.A) | FC |
FITC anti-mouse CD106 | 429 (MVCAM.A) | FC |
LEAF™ Purified anti-mouse CD106 | 429 (MVCAM.A) | FC, IHC-F, IP |
Purified anti-mouse CD106 | 429 (MVCAM.A) | FC,IHC-F,IP |
Alexa Fluor® 488 anti-mouse CD106 | 429 (MVCAM.A) | FC,IHC-F,SB |
Alexa Fluor® 647 anti-mouse CD106 | 429 (MVCAM.A) | FC,IHC-F,3D IHC |
PE anti-mouse CD106 | 429 (MVCAM.A) | FC |
PerCP/Cyanine5.5 anti-mouse CD106 | 429 (MVCAM.A) | FC |
APC anti-mouse CD106 | 429 (MVCAM.A) | FC |
PE/Cyanine7 anti-mouse CD106 | 429 (MVCAM.A) | FC |
Pacific Blue™ anti-mouse CD106 | 429 (MVCAM.A) | FC |
Alexa Fluor® 594 anti-mouse CD106 | 429 (MVCAM.A) | IHC-F |
TotalSeq™-A0226 anti-mouse CD106 | 429 (MVCAM.A) | PG |
Ultra-LEAF™ Purified anti-mouse CD106 | 429 (MVCAM.A) | FC,IHC-F,IP |
TotalSeq™-C0226 anti-mouse CD106 | 429 (MVCAM.A) | PG |
TotalSeq™-B0226 anti-mouse CD106 | 429 (MVCAM.A) | PG |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse CD106
C57BL/6 bone marrow myeloid cells stained with 429 FITC -
FITC anti-mouse CD106
C57BL/6 bone marrow myeloid cells stained with 429 FITC -
LEAF™ Purified anti-mouse CD106
C57BL/6 bone marrow myeloid cells stained with 429 FITC -
Purified anti-mouse CD106
C57BL/6 mouse bone marrow myeloid cells stained with 429 pur... -
Alexa Fluor® 488 anti-mouse CD106
C57BL/6 mouse bone marrow cells stained with 429 Alexa Fluor... C57BL/6 mouse frozen testis section was fixed with 4% parafo... Mice were injected subcutaneously with sheep red blood cells... Mice were injected subcutaneously with sheep red blood cells... -
Alexa Fluor® 647 anti-mouse CD106
C57BL/6 bone marrow myeloid cells stained with 429 Alexa Flu... C57BL/6 mouse frozen testis section was fixed with 4% parafo... Dissected C57/B6 mouse testis was immersed in 4% paraformal... Formalin-fixed, 300 micron-thick mouse testis section was bl... Paraformaldehyde-fixed (4%), 500 µm-thick mouse testis secti... -
PE anti-mouse CD106
C57BL/6 bone marrow cells stained with 429 PE (gated on myel... -
PerCP/Cyanine5.5 anti-mouse CD106
C57BL/6 mouse bone marrow cells stained with CD106 (clone 42... -
APC anti-mouse CD106
C57BL/6 mouse bone marrow cells stained with 429 APC -
PE/Cyanine7 anti-mouse CD106
C57BL/6 bone marrow cells stained with 429 PE/Cyanine7 -
Pacific Blue™ anti-mouse CD106
C57BL/6 bone marrow cells stained with 429 Pacific Blue&trad... C57BL/6 bone marrow cells stained with rat IgG2a isotype con... -
Alexa Fluor® 594 anti-mouse CD106
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Dissected C57/B6 mouse testis was immersed in 4% paraformal... -
TotalSeq™-A0226 anti-mouse CD106
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Ultra-LEAF™ Purified anti-mouse CD106
C57BL/6 mouse bone marrow myeloid cells stained with 429 pur... -
TotalSeq™-C0226 anti-mouse CD106
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TotalSeq™-B0226 anti-mouse CD106
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