- Clone
- 5G6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Sialic acid binding Ig-like lectin 10
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![5G6_APC_1_033012 5G6_APC_1_033012](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/5G6_APC_1_033012.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Human peripheral blood lymphocytes were stained with CD19 FITC and Siglec-10 (clone 5G6) APC (top) or mouse IgG1, κ APC isotype control (bottom). -
Siglec-10 is a lectin that specifically binds α-2,3- or α-2,6-linked sialic acid. It is a member of the Ig-superfamily and is expressed in monocytes, eosinophils, B cells, and a subset of NK cells. Siglec-10 is a single pass transmembrane protein. The extracellular domain contains one V-set Ig-like domain and three C2-set domains; the cytoplasmic domain contains one immunoreceptor tyrosine-based inhibitor motif (ITIM) which, after tyrosine-phosphorylation, recruits SH2-family phosphatases such as SHP-1 and PTPN6, resulting in the blocking of the signal transduction. CD24 and the vascular adhesion protein-1 (VAP-1) have been described as ligands of Siglec-10.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant Siglec-10 fused to human IgG Fc
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Red Laser (633 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Munday J, et al. 2001. Biochem. J. 355:489
- Yokoi H, et al. 2006. Allergy. 61:769
- Kivi E, et al. 2009. Blood 114:5385
- Barkal AA, et al. 2019. Nature. 572(7769):392-396. (Agonist)
- Product Citations
-
- RRID
-
AB_11203899 (BioLegend Cat. No. 347606)
Antigen Details
- Structure
- Lectin, belongs to the Ig-superfamily, has one V-set Ig-like domain and three C2-set domains, a single transmembrane domain and one immunoreceptor tyrosine-based inhibitor motif (ITIM) in the cytoplasmic domain
- Distribution
-
Monocytes, eosinophils, B cells, and a subset of NK cells
- Function
- Cell adhesion, signal transduction
- Interaction
- SHP-1, PTPN6
- Ligand/Receptor
-
CD24, vascular adhesion protein-1 (VAP-1)
- Bioactivity
- Inhibition of signal transduction by recruiting cytoplasmic phosphatases
- Cell Type
- B cells, Eosinophils, Monocytes, NK cells
- Biology Area
- Immunology
- Molecular Family
- Siglec Molecules
- Antigen References
-
1. Chen GY, et al. 2009. Science 323:1722
2. Aizawa H, et al. 2003. Genomics.82:521
3. Kitzig F, et al. 2002. Biochem Biophys Res Commun. 296:355 - Gene ID
- 89790 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- Siglec-10
- Specificity Alt (DOES NOT SHOW ON TDS):
- Siglec-10
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC
- UniProt
- View information about Siglec-10 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Siglec-10 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-human Siglec-10 | 5G6 | FC |
APC anti-human Siglec-10 | 5G6 | FC |
PE/Cyanine7 anti-human Siglec-10 | 5G6 | FC |
TotalSeq™-C1050 anti-human Siglec-10 Antibody | 5G6 | PG |
TotalSeq™-B1050 anti-human Siglec-10 Antibody | 5G6 | PG |
PE/Dazzle™ 594 anti-human Siglec-10 | 5G6 | FC |
Brilliant Violet 421™ anti-human Siglec-10 | 5G6 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
PE anti-human Siglec-10
Human peripheral blood lymphocytes stained with anti-human C... -
APC anti-human Siglec-10
Human peripheral blood lymphocytes were stained with CD19 FI... -
PE/Cyanine7 anti-human Siglec-10
Human peripheral blood lymphocytes were stained with CD19 Br... -
TotalSeq™-C1050 anti-human Siglec-10 Antibody
-
TotalSeq™-B1050 anti-human Siglec-10 Antibody
-
PE/Dazzle™ 594 anti-human Siglec-10
Human peripheral blood lymphocytes were stained with anti-hu... -
Brilliant Violet 421™ anti-human Siglec-10
Human peripheral blood lymphocytes were stained with anti-hu...
Follow Us