- Clone
- 6/40c (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CEACAM8, CD67, CGM6, NCA-95
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![6slash40c_BV421_CD66b_Antibody_102820 6slash40c_BV421_CD66b_Antibody_102820](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/6slash40c_BV421_CD66b_Antibody_102820.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood granulocytes were stained with CD66b (clone 6/40c) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ isotype control (clone MOPC-21) Brilliant Violet 421™ (open histogram). -
Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD66b (clone 6/40c) Brilliant Violet 421™ (blue) at 4°C overnight. Nuclei were counterstained with Helix NP™ Green (green). The image was scanned with a 10X objective and stitched with Metamorph® software.
Cat # | Size | Price | Save |
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392915 | 25 tests | ¥42,460 | |
392916 | 100 tests | ¥94,380 |
CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant soluble human CEACAM8-Fc produced in HEK293 cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Singer, et al. 2014. PLoS One. 9(4):e94106. PMID: 24743304
- Klaile, et al. 2013. Respir Res. 14:85. PMID 23941132
- Product Citations
-
- RRID
-
AB_2888722 (BioLegend Cat. No. 392915)
AB_2888722 (BioLegend Cat. No. 392916)
Antigen Details
- Structure
- Ig superfamily, CEA antigen group, GPI-linked glycoprotein
- Distribution
-
Granulocytes
- Function
- Cell adhesion, neutrophil activation
- Ligand/Receptor
- CD66c
- Cell Type
- Granulocytes
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Kuijpers T, et al. 1993. J Immunol. 151:4934.
- Kuroki M, et al. 1992. J Leuk Biol. 52:551.
- Gene ID
- 1088 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- CD66b
- Specificity Alt (DOES NOT SHOW ON TDS):
- CD66b
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC,IHC-P
- UniProt
- View information about CD66b on UniProt.org
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
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It is lot-specific. On average it ranges between 2-4.
Other Formats
View All CD66b Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD66b | 6/40c | FC,IHC-P,SB |
PE anti-human CD66b | 6/40c | FC |
TotalSeq™-A0166 anti-human CD66b | 6/40c | PG |
Alexa Fluor® 594 anti-human CD66b | 6/40c | IHC-P |
TotalSeq™-C0166 anti-human CD66b | 6/40c | PG |
Alexa Fluor® 647 anti-human CD66b | 6/40c | IHC-P,FC |
TotalSeq™-B0166 anti-human CD66b | 6/40c | PG |
PE/Fire™ 640 anti-human CD66b Antibody | 6/40c | FC |
Brilliant Violet 421™ anti-human CD66b Antibody | 6/40c | FC,IHC-P |
TotalSeq™-D0166 anti-human CD66b | 6/40c | PG |
TotalSeq™-Bn0166 anti-human CD66b | 6/40c | SB |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD66b
Human peripheral blood granulocytes was stained with CD66b (... Human paraffin-embedded spleen tissue slices were prepared w... SeqIF™ (sequential immunofluorescence) staining on COMET™ of... -
PE anti-human CD66b
Human peripheral blood granulocytes were stained with CD66b ... -
TotalSeq™-A0166 anti-human CD66b
-
Alexa Fluor® 594 anti-human CD66b
Human paraffin-embedded spleen tissue slices were prepared w... -
TotalSeq™-C0166 anti-human CD66b
-
Alexa Fluor® 647 anti-human CD66b
Human paraffin-embedded spleen tissue slices were prepared w... Human peripheral blood granulocytes were stained with CD66b ... -
TotalSeq™-B0166 anti-human CD66b
-
PE/Fire™ 640 anti-human CD66b Antibody
Human peripheral blood granulocytes were stained with anti-h... -
Brilliant Violet 421™ anti-human CD66b Antibody
Human peripheral blood granulocytes were stained with CD66b ... Human paraffin-embedded spleen tissue slices were prepared w... -
TotalSeq™-D0166 anti-human CD66b
-
TotalSeq™-Bn0166 anti-human CD66b
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