- Clone
- W16197A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- ACTB, Actin, cytoplasmic 1, Beta-actin
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W16197A_DBHRP_beta-actin_Antibody_032918 W16197A_DBHRP_beta-actin_Antibody_032918](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W16197A_DBHRP_beta-actin_Antibody_032918.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Whole cell lysates (15 µg protein) from HeLa was resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:10,000 diluted (0.01 µg/mL), 1:40,000 diluted (0.0025 µg/mL),1:100,000 diluted (0.001 µg/mL) Direct-Blot™ HRP anti-β-actin Antibody, clone W16197A, and 1:10,000 diluted (0.01 µg/mL) purified anti-β-actin Antibody, clone W16197A (Cat. No. 664801) (upper). Proteins were visualized using an 1:3000 diluted HRP anti rat-IgG secondary antibody (Cat. No.405405) for purified anti-β-actin Antibody and chemiluminescence detection. Ponceau S staining was used as loading control (lower). Lane M: MW ladder.
Cat # | Size | Price | Save |
---|---|---|---|
664803 | 25 µL | ¥22,220 | |
664804 | 100 µL | ¥74,800 |
β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat, Zebrafish, Drosophila
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Human ACTB peptide (1-13 a.a.).
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.001 - 0.01 µg per ml (1:10,000 - 1:100,000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.
25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution. - Application Notes
-
Reactivity to Drosophila was only verified with the purified format.
- Product Citations
-
- RRID
-
AB_2810693 (BioLegend Cat. No. 664803)
AB_2728496 (BioLegend Cat. No. 664804)
Antigen Details
- Structure
- 375 amino acids with a predicted molecular weight of 42 kD.
- Distribution
-
Cytoplasm
- Function
- Actins are highly conserved proteins that are involved in cell motility.
- Interaction
- Interacts with CPNE1 and CPNE4, ERBB2, GCSAM, TBC1D21.
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Microfilaments
- Antigen References
-
1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687. - Gene ID
- 60 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- beta-actin
- Specificity Alt (DOES NOT SHOW ON TDS):
- β-actin
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB
- UniProt
- View information about beta-actin on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All β-actin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-β-actin | W16197A | WB |
Direct-Blot™ HRP anti-β-actin | W16197A | WB |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-β-actin
Total lysates (15 µg protein) from HeLa, NIH3T3, rat brain a... Total lysates (15 µg protein) from HeLa was resolved by elec... Western blot of purified anti-β-actin antibody (clone W16197... -
Direct-Blot™ HRP anti-β-actin
Whole cell lysates (15 µg protein) from HeLa was resolved by...
Follow Us