- Clone
- Poly6341 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Caspase 3 preproprotein, Casp3, cpp32, Apopain, Yama, PARP cleavage protease, SREBP cleavage activity 1
- Isotype
- Rabbit Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Western blot analysis of extracts from Hela cells, using anti-caspase-3, Poly6341.
Cat # | Size | Price | Save |
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634101 | 125 µl (5 Western Blots) | ¥22,400 |
Caspase 3 is a member of the peptidase family C14. This cytoplasmic caspase functions as a heterotetramer and has two isoforms (one acts as a dominant negative inhibitor). The pro-form of this caspase is approximately 32 kD. Caspase 3 is involved in the caspase activation cascade responsible for apoptosis execution. Caspase 3 is inhibited by c-IAP1, c-IAP2, XIAP, survivin, and Livin and activated by Caspase 6, -8 , and -10, granzyme B, Apaf-1 by cleavage into large p17 and small p12 subunits. Caspase 3 has been shown to cleave PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topoisomerase I, vimentin, Rb, lamin B, mdm2, and β-catenin. The poly6341 antibody has been shown to be useful for western blotting, immunoprecipitation and immunohistochemistry of human Human caspase-3 precursor.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Peptide mapping to the amino terminal domain of human caspase-3 prescursor
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% gelatin.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. DO NOT FREEZE.
- Application
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WB - Quality tested
IP - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. Western blotting suggested working dilution(s): Use 25µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This product may contain other non-IgG subtypes. - Product Citations
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- RRID
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AB_2069874 (BioLegend Cat. No. 634101)
Antigen Details
- Structure
- Peptidase family C14, heterotetramer. Two isoforms, pro-Casp3 32 kD
- Interaction
- Active heterodimers between large p17 subunit and small p11 subunit of Casp7 and vice versa. Cleaves PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topo
- Bioactivity
- Involved in caspase activation cascade responsible for apoptosis execution. Cleaves/activates SREBPs, Casp6, Casp7, Casp9
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
- Antigen References
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1. Goldberg Y, et al. 1996. Nat. Genet. 13:442.
2. Fernandes-Alnemri T, et al. 1996. P. Natl. Acad. Sci. USA 93:7464.
3. Slee E, et al. 2001. J. Biol. Chem. 276:7320.
4. Woo M, et al. 2003. Nat. Immunol. 4:1016. - Regulation
- Inhibited by dominant negative isoform, c-IAP1, c-IAP2, XIAP, survivin, Livin. Activated when cleaved by Casp6, -8, -10, granzyme B, Apaf-1 into large p17 and small p12 subunits
- Gene ID
- 12367 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- Caspase-3
- Specificity Alt (DOES NOT SHOW ON TDS):
- Caspase-3
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB,IP
- UniProt
- View information about Caspase-3 on UniProt.org
Related FAQs
Other Formats
View All Caspase-3 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Caspase-3 | Poly6341 | WB,IP |
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Purified anti-Caspase-3
Western blot analysis of extracts from Hela cells, using ant...
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