- Clone
- A161A1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T4/Leu-3
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A161A1_PECy7_CD4_Antibody_FC_052815 A161A1_PECy7_CD4_Antibody_FC_052815](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A161A1_PECy7_CD4_Antibody_FC_052815.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with CD4 (clone A161A1) PE/Cyanine7 (filled histogram) or rat IgG2b, κ PE/Cyanine7 isotype control (open histogram).
Cat # | Size | Price | Save |
---|---|---|---|
357409 | 25 tests | ¥33,660 | |
357410 | 100 tests | ¥68,420 |
CD4, also known as T4/Leu-3, is a 55 kD single-chain type I transmembrane glycoprotein and member of the immunoglobulin superfamily. It is expressed on most thymocytes, helper T cells, type II NKT cells, and monocytes/macrophages. CD4 is part of the TCR/CD3 complex, binds to β2 domain from the MHC class II molecule, and participates in TCR signal transduction. CD4 is the receptor of IL-16 and is a coreceptor for the human immunodeficiency virus (HIV) and human herpes virus 7 (HHV-7).
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Human CD4 T cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- Product Citations
-
- RRID
-
AB_2565661 (BioLegend Cat. No. 357409)
AB_2565662 (BioLegend Cat. No. 357410)
Antigen Details
- Structure
- 55 kD, type I transmembrane glycoprotein, member of the Ig superfamily
- Distribution
-
Helper T cells, subset of thymocytes, monocytes/macrophages, type II NKT cells
- Interaction
- Part of the TCR/CD3 complex
- Ligand/Receptor
- β2 domain from the MHC class II
- Cell Type
- T cells, Monocytes, Macrophages, NKT cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Zhu J, et al. 2010. Annu Rev. Immunol. 28:445.
2. Vignali DA. 2010. J. Immunol. 184:5933.
3. Zhou L, et al. 2009. Immunity 30:646.
4. Singer A, et al. 2008. Nat. Rev. Immunol. 8:788.
5. Zhu J and Paul WE. 2008. Blood 112:1557. - Gene ID
- 920 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- CD4
- Specificity Alt (DOES NOT SHOW ON TDS):
- CD4
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC
- UniProt
- View information about CD4 on UniProt.org
Related FAQs
- I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
- TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Other Formats
View All CD4 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD4 | A161A1 | FC |
PE anti-human CD4 | A161A1 | FC |
FITC anti-human CD4 | A161A1 | FC |
PerCP/Cyanine5.5 anti-human CD4 | A161A1 | FC |
APC anti-human CD4 | A161A1 | FC |
PE/Cyanine7 anti-human CD4 | A161A1 | FC |
PE/Dazzle™ 594 anti-human CD4 | A161A1 | FC |
APC/Cyanine7 anti-human CD4 | A161A1 | FC |
Alexa Fluor® 700 anti-human CD4 | A161A1 | FC |
Brilliant Violet 510™ anti-human CD4 | A161A1 | FC |
APC/Fire™ 750 anti-human CD4 | A161A1 | FC |
Alexa Fluor® 647 anti-human CD4 | A161A1 | FC |
Biotin anti-human CD4 | A161A1 | FC |
Brilliant Violet 421™ anti-human CD4 | A161A1 | FC |
PE/Cyanine5 anti-human CD4 | A161A1 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD4
Human peripheral blood lymphocytes were stained with purifie... -
PE anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
FITC anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
PerCP/Cyanine5.5 anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
APC anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
PE/Cyanine7 anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
PE/Dazzle™ 594 anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
APC/Cyanine7 anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
Alexa Fluor® 700 anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
Brilliant Violet 510™ anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (c... -
APC/Fire™ 750 anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
Alexa Fluor® 647 anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
Biotin anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
Brilliant Violet 421™ anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
PE/Cyanine5 anti-human CD4
Human peripheral blood lymphocytes were stained with anti-hu...
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