- Clone
- 9F (See other available formats)
- Regulatory Status
- RUO
- Other Names
- DPYSL2, Collapsin Response Mediator Protein 2, Dihydropyrimidinase-Related Protein 2
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![9F_Purified_CRMP2_Antibody_1_072519 9F_Purified_CRMP2_Antibody_1_072519](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/9F_Purified_CRMP2_Antibody_1_072519.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-CRMP2 antibody (clone 9F). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-CRMP2 antibody (clone 9F) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-CRMP2 antibody (clone 9F) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-CRMP2 antibody (clone 9F) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # | Size | Price | Save |
---|---|---|---|
869001 | 25 µg | ¥23,320 | |
869002 | 100 µg | ¥60,720 |
CRMP2 is a ubiquitously expressed, microtubule-associated protein. In the central nervous system, CRMP2 forms homotetramers or heterotetramers with other CRMP proteins during development and regulates axonal growth and guidance, neuronal growth cone collapse, and migration. The C-terminal sequence of CRMP2 is highly homologous to that of Tau protein. Phosphorylation of CRMP2 alters its interaction with microtubules and affects its protein scaffolding function. Similar to Tau, CRMP2 is a biochemically prominent component of Neurofibrillary tangles. These finding suggest that CRMP2 may be an etiologically significant protein and a potential therapeutic target for Alzheimer’s disease.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 1.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2814627 (BioLegend Cat. No. 869001)
AB_2814628 (BioLegend Cat. No. 869002)
Antigen Details
- Structure
- CRMP2 is a 572 amino acid protein with predicted and observed molecular mass of ~ 63 kD.
- Distribution
-
Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Cytosol, plasma membrane, and cytoskeleton - Function
- CRMP2 forms homotetramers or heterotetramers with other CRMP proteins, and regulates axonal growth and guidance, neuronal growth cone collapse, and migration.
- Interaction
- CRMP2 forms homotetramers and heterotetramers with CRMP1, DPYSL3, DPYSL4 or DPYSL5. It interacts with HTR4, CLN6, and, MICALL1.
- Cell Type
- Astrocytes, Neurons, Oligodendrocytes
- Biology Area
- Cell Motility/Cytoskeleton/Structure, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
- Molecular Family
- Cytoskeletal Proteins, Microfilaments, Microtubules, Scaffold Proteins
- Antigen References
-
- Hensley K, et al. 2016. J Alzheimers dis. 53(1):1
- Quach TT, et al. 2015. Mol Psychiatry. 20(9):1037
- Ip JP, et la. 2014. Neuroscientist. 20(6):589
- Gene ID
- 1808 View all products for this Gene ID 12934 View all products for this Gene ID 25416 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- CRMP2
- Specificity Alt (DOES NOT SHOW ON TDS):
- CRMP2
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB,IHC-P
- UniProt
- View information about CRMP2 on UniProt.org
Other Formats
View All CRMP2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-CRMP2 | 9F | WB,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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