- Clone
- HIT2 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- III 155
- Other Names
- T10, ADP-ribosyl cyclase
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![HIT2_Purified_CD38_CyTOF_112113 HIT2_Purified_CD38_CyTOF_112113](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/HIT2_Purified_CD38_CyTOF_112113.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 167Er-anti-CD38 (HIT2). Data provided by DVS Sciences.
CD38 is a 45 kD type II transmembrane glycoprotein also known as T10. It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such as brain, muscles, and kidney). In humans, it is expressed at high levels on plasma cells and activated T and B cells. By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation, adhesion, and the metabolism of cADPR and NAADP. CD31 is the ligand of CD38.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee, Horse, Cow
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF® - Verified - Recommended Usage
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This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6 and spatial biology (IBEX)10,11.
- Additional Product Notes
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Maxpar® is a registered trademark of Standard BioTools Inc.
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Application References
(PubMed link indicates BioLegend citation) -
- Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
- Dieu M. 1998. J. Exp. Med. 188:373.
- Esser M, et al. 2001. J. Virol. 75:6173.
- Jeannin P, et al. 1999. J. Immunol. 162:2044.
- Kapsogeorgou EK, et al. 2001. J. Immunol. 166:3107.
- van der Voort R, et al. 1997. J. Exp. Med. 185:2121. (IHC)
- Bende RJ, et al. 2003. Am. J. Pathol. 162:105.
- Lehner M, et al. 2008. J. Leukoc. Biol. 83:883. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
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AB_2562819 (BioLegend Cat. No. 303535)
Antigen Details
- Structure
- ADP-ribosyl cyclase, ectoenzyme, type II glycoprotein, 45 kD
- Distribution
-
T cells, B cells, NK, myeloid, plasma, and dendritic cells
- Function
- Ecto-ADP-ribosyl cyclase, calcium signaling, cell activation
- Ligand/Receptor
- CD31, hyaluronic acid
- Cell Type
- B cells, Dendritic cells, NK cells, Plasma cells, T cells
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Ferrero E, et al. 1999. J. Leukoc. Biol. 65:151.
2. Lund F, et al. 1995. Immunol. Today 16:469. - Gene ID
- 952 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- CD38
- Specificity Alt (DOES NOT SHOW ON TDS):
- CD38
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC,CyTOF®
- UniProt
- View information about CD38 on UniProt.org
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
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We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
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We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
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We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
-
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All CD38 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 APC -
FITC anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 FITC -
PE anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 PE -
PE/Cyanine5 anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 PE/Cyan... -
Purified anti-human CD38
Human peripheral blood lymphocytes stained with purified HIT... -
Alexa Fluor® 488 anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 Alexa F... -
Alexa Fluor® 647 anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 Alexa F... -
PE/Cyanine7 anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 PE/Cyan... -
Biotin anti-human CD38
Human peripheral blood lymphocytes stained with biotinylated... -
PerCP anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 PerCP -
PerCP/Cyanine5.5 anti-human CD38
Human peripheral blood lymphocytes were stained with CD38 (c... -
Alexa Fluor® 700 anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 Alexa F... Confocal image of human lymph node sample acquired using the... -
Brilliant Violet 421™ anti-human CD38
Human peripheral blood lymphocytes were stained with CD38 (c... -
Brilliant Violet 711™ anti-human CD38
Human peripheral blood lymphocytes were stained with CD38 (c... -
Brilliant Violet 785™ anti-human CD38
Human peripheral blood lymphocytes were stained with CD38 (c... -
Brilliant Violet 605™ anti-human CD38
Human peripheral blood lymphocytes were stained with CD38 (c... -
APC/Cyanine7 anti-human CD38
Human peripheral blood lymphocytes were stained with CD38 (c... -
Purified anti-human CD38 (Maxpar® Ready)
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 167Er-an... -
PE/Dazzle™ 594 anti-human CD38
Human peripheral blood granulocytes were stained with CD38 (... -
PE anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
Brilliant Violet 510™ anti-human CD38
Human peripheral blood lymphocytes were stained with CD38 (c... -
FITC anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
PE/Dazzle™ 594 anti-human CD38
Typical results from human peripheral blood granulocytes sta... -
TotalSeq™-A0389 anti-human CD38
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TotalSeq™-C0389 anti-human CD38
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APC/Fire™ 750 anti-human CD38
Human peripheral blood lymphocytes were stained with CD38 (C... -
TotalSeq™-B0389 anti-human CD38
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APC/Fire™ 810 anti-human CD38
Human peripheral blood lymphocytes were stained with CD38 (c... -
Spark NIR™ 685 anti-human CD38 Antibody
Human peripheral blood lymphocytes were stained with CD38 (c... -
TotalSeq™-D0389 anti-human CD38
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PerCP/Cyanine5.5 anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
APC anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 750 anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
PE/Cyanine7 anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
GMP PE anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
GMP FITC anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
Pacific Blue™ anti-human CD38
Human peripheral blood lymphocytes were stained with anti-hu... -
Pacific Blue™ anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
GMP APC/Fire™ 750 anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
GMP PE/Dazzle™ 594 anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
GMP APC anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
GMP PE/Cyanine7 anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
Spark Blue™ 515 anti-human CD38
Human peripheral blood cells were surface stained with (left... -
GMP Pacific Blue™ anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
Spark Violet™ 423 anti-human CD38
Human peripheral blood lymphocytes were stained with anti-hu... -
Brilliant Violet 650™ anti-human CD38
Human peripheral blood lymphocytes were stained with anti-hu...
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