- Clone
- A7R34 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-7 receptor α chain, IL-7Rα
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_A7R34_A488_102809 A_A7R34_A488_102809](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A_A7R34_A488_102809.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
C57BL/6 mouse splenocytes stained with CD3 (145-2C11) APC and A7R34 Alexa Fluor® 488 (top) or rat IgG2a, κ Alexa Fluor® 488 isotype control (bottom). -
Cat # | Size | Price | Save |
---|---|---|---|
135017 | 25 µg | ¥27,280 | |
135018 | 100 µg | ¥64,460 |
CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- IL-7Ra-IgG1 fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
A7R34 is able to block clone SB/199 binding to IL-7R.
-
Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
-
AB_1937206 (BioLegend Cat. No. 135017)
AB_1937205 (BioLegend Cat. No. 135018)
Antigen Details
- Structure
- Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
- Distribution
-
Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
- Function
- T cell and immature B cell proliferation and development
- Ligand/Receptor
- IL-7
- Cell Type
- B cells, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. Okuno Y, et al. 2001. P. Natl. Acad. Sci. USA 99:6246.
3. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403. - Gene ID
- 16197 View all products for this Gene ID
- UniProt
- View information about CD127 on UniProt.org
Related FAQs
Other Formats
View All CD127 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-mouse CD127 (IL-7Rα)
C57BL/6 mouse splenocytes stained with rat IgG2a (RTK2758) P... C57BL/6 mouse splenocytes stained with A7R34 PE and CD3 (145... -
FITC anti-mouse CD127 (IL-7Rα)
C57BL/6 splenocytes stained with CD3 (145-2C11) PE and A7R34... -
PE anti-mouse CD127 (IL-7Rα)
C57BL/6 mouse splenocytes stained with rat IgG2a (RTK2758) P... C57BL/6 mouse splenocytes stained with A7R34 PE and CD3 (145... -
APC anti-mouse CD127 (IL-7Rα)
C57BL/6 splenocytes stained with CD3 (145-2C11) PE and A7R34... -
PE/Cyanine7 anti-mouse CD127 (IL-7Rα)
C57BL/6 mouse splenocytes stained with CD3ε (clone 1... -
PE/Cyanine5 anti-mouse CD127 (IL-7Rα)
C57BL/6 mouse splenocytes stained with CD3 (clone 145-2C11) ... -
Alexa Fluor® 488 anti-mouse CD127 (IL-7Rα)
C57BL/6 mouse splenocytes stained with CD3 (145-2C11) APC an... -
Alexa Fluor® 647 anti-mouse CD127 (IL-7Rα)
Mouse splenocytes stained with CD3 FITC and A7R34 Alexa Fluo... -
PerCP/Cyanine5.5 anti-mouse CD127 (IL-7Rα)
C57BL/6 mouse splenocytes stained with CD3 (17A2) PE and A7R... -
Biotin anti-mouse CD127 (IL-7Rα)
C57BL/6 mouse splenocytes stained with CD3ε (145-2C11) APC ... -
Brilliant Violet 421™ anti-mouse CD127 (IL-7Rα)
C57BL/6 mouse splenocytes were stained with CD3ε FITC and CD... -
Brilliant Violet 605™ anti-mouse CD127 (IL-7Rα)
C57BL/6 mouse splenocytes were stained with CD3ε FIT... -
Purified anti-mouse CD127 (IL-7Rα) (Maxpar® Ready)
C57BL/6 mouse splenocytes stained with 169Tm-anti-TCRbeta (H... -
PE/Dazzle™ 594 anti-mouse CD127 (IL-7Rα)
C57BL/6 mouse splenocytes were stained with CD3ε FIT... -
Brilliant Violet 510™ anti-mouse CD127 (IL-7Rα)
C57BL/6 mouse splenocytes were stained with CD3ε APC... -
Brilliant Violet 711™ anti-mouse CD127 (IL-7Rα)
C57BL/6 mouse splenocytes were stained with CD3ε APC... -
Brilliant Violet 785™ anti-mouse CD127 (IL-7Rα)
C57BL/6 mouse splenocytes were stained with CD3ε APC... -
APC/Cyanine7 anti-mouse CD127 (IL-7Rα)
C57BL/6 mouse splenocytes were stained with CD3 FITC and CD1... -
Brilliant Violet 650™ anti-mouse CD127 (IL-7Rα)
C57BL/6 mouse splenocytes were stained with CD3ε FIT... -
TotalSeq™-A0198 anti-mouse CD127 (IL-7Rα)
-
TotalSeq™-C0198 anti-mouse CD127 (IL-7Rα)
-
Ultra-LEAF™ Purified anti-mouse CD127 (IL-7Rα)
-
TotalSeq™-B0198 anti-mouse CD127 (IL-7Rα)
Follow Us