- Clone
- MRG2b-85 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin G2b
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![MRG2b-85_A488_IgG2b_Antibody_FC_0791118 MRG2b-85_A488_IgG2b_Antibody_FC_0791118](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/MRG2b-85_A488_IgG2b_Antibody_FC_091118.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with purified CD3 (clone 17A2) followed by anti-rat IgG2b (clone MRG2b-85) Alexa Fluor® 488. -
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of CD3 (clone 17-A2) purified and 10 µg/ml of B220 (Clone RA3-6B2) Alexa Fluor® 594 (red) overnight at 4°C, followed by 2.5 µg/ml of anti-rat IgG2b (Clone MRG2b-85) Alexa Fluor® 488 (green) for 2 hours at room temperature. The image was captured by 10X objective.
Cat # | Size | Price | Save |
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408210 | 25 µg | ¥23,320 | |
408211 | 100 µg | ¥44,000 |
The MRG2b-85 monoclonal antibody reacts with rat immunoglobulin G2b (IgG2b) in all tested rat strains (Lou, Lou/Ws1/M, Lewis, Wistar, DA, Sprague-Dawley). The MRG2b-85 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
Product DetailsProduct Details
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Mixed rat Igs
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
IHC-F - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Blue Laser (488 nm)
- Product Citations
-
- RRID
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AB_2715913 (BioLegend Cat. No. 408210)
AB_2715886 (BioLegend Cat. No. 408211)
Antigen Details
- Gene ID
- NA
- UniProt
- View information about IgG2b on UniProt.org
Related FAQs
Other Formats
View All IgG2b Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-rat IgG2b | MRG2b-85 | ELISA,FC |
Biotin anti-rat IgG2b | MRG2b-85 | ELISA,FC |
FITC anti-rat IgG2b | MRG2b-85 | FC |
Alexa Fluor® 594 anti-rat IgG2b | MRG2b-85 | IHC-F,ICC |
Alexa Fluor® 488 anti-rat IgG2b | MRG2b-85 | FC,IHC-F |
Alexa Fluor® 647 anti-rat IgG2b | MRG2b-85 | IHC-F,ICC |
PE anti-rat IgG2b | MRG2b-85 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-rat IgG2b
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Biotin anti-rat IgG2b
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FITC anti-rat IgG2b
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Alexa Fluor® 594 anti-rat IgG2b
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... HeLa cells were fixed with 4% paraformaldehyde for 10 minute... -
Alexa Fluor® 488 anti-rat IgG2b
C57BL/6 mouse splenocytes were stained with purified CD3 (cl... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
Alexa Fluor® 647 anti-rat IgG2b
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... HeLa cells were fixed with 4% paraformaldehyde for 10 minute... -
PE anti-rat IgG2b
C57BL/6 splenocytes stained with purified anti-mouse CD3 (cl...
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