- Clone
- dG9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PRF1, P1, PFP, HPLH2
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![dG9_A594_Perforin_Antibody_102820 dG9_A594_Perforin_Antibody_102820](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/dG9_A594_Perforin_Antibody_102820.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood mononuclear cells were fixed with 2% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes at room temperature. Then the cells were stained with 5 µg/ml Perforin (clone dG9) Alexa Fluor® 594 (red) and CD8 (clone HIT8a) Alexa Fluor® 488 (green) for one hour. The nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Cow
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified granules from the human lymphoma cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Clone dG9 primarily recognizes perforin associated with cytotoxic granules9. Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular flow cytometric analysis and immunofluorescence microscopy5,7, and immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections1,4.
Does not cross-react with mouse1. -
Application References
(PubMed link indicates BioLegend citation) -
- Hameed A, et al. 1992. Am. J. Pathol. 140:1025. (IHC)
- Schaerli P, et al. 2004. J. Exp. Med. 199:1265.
- Watanabe N, et al. 1997. Blood 90:3662.
- Mauad T, et al. 2004. Pediatr. Pulmonol. 38:233. (IHC)
- Barrat FJ, et al. 1999. P. Natl. Acad. Sci. USA 96:8645. (IF)
- Chen H, et al. 2005. J. Immunol. 175:591.
- Bryceson YT, et al. 2007. Blood doi:10.1182/blood-2007-02-074468. (IF)
- Wood SM, et al. 2009. Blood 114:4117. PubMed
- Makedonas G, et al. 2010. PLoS Pathog. 6:e1000798.
- RRID
-
AB_2566802 (BioLegend Cat. No. 308124)
Antigen Details
- Structure
- 70 kD
- Distribution
-
CTL, NK (cytoplasmic granules)
- Function
- Mediates targeted cell lysis
- Cell Type
- NK cells, T cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361.
2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735. - Gene ID
- 5551 View all products for this Gene ID
- UniProt
- View information about Perforin on UniProt.org
Other Formats
View All Perforin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
FITC anti-human Perforin | dG9 | ICFC |
PE anti-human Perforin | dG9 | ICFC |
Purified anti-human Perforin | dG9 | ICFC,IP,ICC,IHC |
Alexa Fluor® 488 anti-human Perforin | dG9 | ICFC |
Alexa Fluor® 647 anti-human Perforin | dG9 | ICFC |
APC anti-human Perforin | dG9 | ICFC |
Pacific Blue™ anti-human Perforin | dG9 | ICFC |
PerCP/Cyanine5.5 anti-human Perforin | dG9 | ICFC |
Brilliant Violet 510™ anti-human Perforin | dG9 | ICFC |
Brilliant Violet 421™ anti-human Perforin | dG9 | ICFC |
Alexa Fluor® 594 anti-human Perforin | dG9 | ICC |
APC/Cyanine7 anti-human Perforin | dG9 | ICFC |
PE/Cyanine7 anti-human Perforin | dG9 | ICFC |
Brilliant Violet 711™ anti-human Perforin | dG9 | ICFC |
PE/Dazzle™ 594 anti-human Perforin | dG9 | ICFC |
TotalSeq™-B0044 anti-human Perforin | dG9 | ICPG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-human Perforin
Whole blood lymphocytes stained intracellularly with dG9 FIT... -
PE anti-human Perforin
Human peripheral blood lymphocytes intracellularly stained w... -
Purified anti-human Perforin
Whole blood lymphocytes stained intracellularly with dG9 FIT... -
Alexa Fluor® 488 anti-human Perforin
Human peripheral blood lymphocytes intracellularly stained w... -
Alexa Fluor® 647 anti-human Perforin
Human peripheral blood mononuclear cells intracellularly sta... -
APC anti-human Perforin
Human peripheral blood lymphocytes intracellularly stained w... -
Pacific Blue™ anti-human Perforin
Human peripheral blood lymphocytes were surface stained with... -
PerCP/Cyanine5.5 anti-human Perforin
Human peripheral blood lymphocytes were surface stained with... -
Brilliant Violet 510™ anti-human Perforin
Human peripheral blood lymphocytes were surface stained with... -
Brilliant Violet 421™ anti-human Perforin
Human peripheral blood lymphocytes were surface stained with... -
Alexa Fluor® 594 anti-human Perforin
Human peripheral blood mononuclear cells were fixed with 2% ... -
APC/Cyanine7 anti-human Perforin
Human peripheral blood lymphocytes were surface stained with... -
PE/Cyanine7 anti-human Perforin
Human peripheral blood lymphocytes were surface stained with... -
Brilliant Violet 711™ anti-human Perforin
Human peripheral blood lymphocytes were surface stained with... -
PE/Dazzle™ 594 anti-human Perforin
Human peripheral blood lymphocytes were surface stained wit... -
TotalSeq™-B0044 anti-human Perforin
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