- Clone
- 1E10A15 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Nuclear receptor subfamily 4, group A, member 1 (NR4A1), GFRP, HMR, Testicular receptor 3 (TR3)
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1E10A15_A594_Nur77_ICC_033114 1E10A15_A594_Nur77_ICC_033114](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/1E10A15_A594_Nur77_ICC_033114.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml anti-Nur77 (clone 1E10A15) Alexa Fluor® 594 (red) in blocking buffer, overnight at 4°C, followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
Nur77, a transcription factor belonging to a superfamily of orphan nuclear receptors, has been shown to activate a subset of genes containing NGFI-B response element (NBRE). Nur77 expression is upregulated in response to stimuli of numerous growth factors, mitogens, and inflammatory substances, indicating that Nur77 has regulatory roles in cell growth and inflammation. Nur77 has also been shown to participate in programmed cell death through mitochondrial targeting and interaction with Bcl-2. In the endocrine system, Nur77 has been reported to participate in regulating production of steroidogenic enzymes. Nur77 and NOR-1 double knockout mice develop acute myeloid leukemia, suggesting that Nur77 plays a role in regulating differentiation of myeloid progenitor cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial human Nur77 recombinant protein (1-266 aa)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - RRID
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AB_2563321 (BioLegend Cat. No. 653504)
Antigen Details
- Structure
- 64 kD protein containing a DNA-binding domain, two zinc finger domain, and a potential ligand-binding domain
- Distribution
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Nucleus
- Function
- Nur77 is a transcription factor that mediates various physiological stimuli, such as growth factors, hormones, inflammatory, and energy metabolic signals. Nur77 plays roles in many important biological processes including cell growth, differentiation, and apoptosis.
- Interaction
- Interacts with GADD45GIP1 and Bcl-2.
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Transcription Factors
- Molecular Family
- Nuclear Markers, Steroid Receptors/Nuclear Receptors
- Antigen References
-
1. Nakai A, et al. 1990. Mol. Endocrinol. 4:1438.
2. Han YH, et al. 2006. Oncogene 25:2974.
3. Li M, et al. 2010. Mol. Cell. Endocrinol. 319:39.
4. Pearen MA, et al. 2010. Mol. Endocrinol. 24:1891.
5. Moll UM, et al. 2006. Oncogene 25:4725.
6. Fassett MS, et al. 2012. Proc. Natl. Acad. Sci. USA 109:3891. - Gene ID
- 3164 View all products for this Gene ID
- UniProt
- View information about Nur77 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Nur77 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Nur77 | 1E10A15 | WB,ICC |
Alexa Fluor® 594 anti-Nur77 | 1E10A15 | ICC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-Nur77
Whole cell extracts (15 µg protein) from serum starved HeLa ... -
Alexa Fluor® 594 anti-Nur77
HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 ...
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