- Clone
- 2/40/15 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Tyrosine 3-monooxygenase, tyrosine 3-hydroxylase
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![2-40-15_A594_Tyrosine_Hydroxylase_Antibody_1_021119.png 2-40-15_A594_Tyrosine_Hydroxylase_Antibody_1_021119.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/2-40-15_A594_Tyrosine_Hydroxylase_Antibody_1_021119.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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IHC staining of Alexa Fluor® 594 anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded human Substantia Nigra tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm -
IHC staining of Alexa Fluor® 594 anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm -
IHC staining of Alexa Fluor® 594 anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # | Size | Price | Save |
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818003 | 25 µg | ¥33,660 | |
818004 | 100 µg | ¥71,060 |
Tyrosine hydroxylase, also known as tyrosine 3-monooxygenase, is the enzyme that converts amino acid L-Tyrosine to L-3, 4-Dihydroxyphenylalanine (L-DOPA). L-DOPA is a precursor to dopamine, which is then converted to norepinephrine and epinephrine (adrenaline). Dopamine, norepinephrine and epinephrine are all important neurotransmitters. Alterations in the activity of tyrosine hydroxylase may be involved in a number of neurological diseases such as Parkinson's and schizophrenia.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against purified Tyrosine Hydroxylase from rat Pheochromocytoma tumor.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-P - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.2 - 1 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
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This antibody is effective in immunoblotting (WB) and formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P).
This antibody is less reactive in human tissue in Western Blot. - Product Citations
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- RRID
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AB_2801150 (BioLegend Cat. No. 818003)
AB_2801151 (BioLegend Cat. No. 818004)
Antigen Details
- Structure
- Expected MW: 65 kD
- Cell Type
- Dopaminergic Neurons, Neural Stem Cells, Neurons
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
- Molecular Family
- Enzymes and Regulators, Presynaptic proteins
- Gene ID
- 25085 View all products for this Gene ID
- UniProt
- View information about Tyrosine Hydroxylase on UniProt.org
Other Formats
View All Tyrosine Hydroxylase Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Tyrosine Hydroxylase | 2/40/15 | IHC-P,WB |
Alexa Fluor® 594 anti-Tyrosine Hydroxylase | 2/40/15 | IHC-P |
Alexa Fluor® 647 anti-Tyrosine Hydroxylase | 2/40/15 | IHC-P |
Alexa Fluor® 488 anti-Tyrosine Hydroxylase | 2/40/15 | IHC-P |
Spark YG™ 570 anti-Tyrosine Hydroxylase | 2/40/15 | IHC-P |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Tyrosine Hydroxylase
IHC staining of purified anti-Tyrosine Hydroxylase antibody ... IHC staining of purified anti-Tyrosine Hydroxylase antibody ... IHC staining of purified anti-Tyrosine Hydroxylase antibody ... IHC staining of purified anti-Tyrosine Hydroxylase antibody... IHC staining of purified anti-Tyrosine Hydroxylase antibody ... Western blot of purified anti-Tyrosine Hydroxylase antibody ... -
Alexa Fluor® 594 anti-Tyrosine Hydroxylase
IHC staining of Alexa Fluor® 594 anti-Tyrosine Hydroxylase a... IHC staining of Alexa Fluor® 594 anti-Tyrosine Hydroxylase a... IHC staining of Alexa Fluor® 594 anti-Tyrosine Hydroxylase a... -
Alexa Fluor® 647 anti-Tyrosine Hydroxylase
IHC staining of Alexa Fluor® 647 anti-Tyrosine Hydroxylase a... IHC staining of Alexa Fluor® 647 anti-Tyrosine Hydroxylase a... IHC staining of Alexa Fluor® 647 anti-Tyrosine Hydroxylase a... IHC staining of Alexa Fluor® 647 anti-Tyrosine Hydroxylase a... -
Alexa Fluor® 488 anti-Tyrosine Hydroxylase
IHC staining of Alexa Fluor® 488 anti-Tyrosine Hydroxylase a... IHC staining of Alexa Fluor® 488 anti-Tyrosine Hydroxylase a... IHC staining of Alexa Fluor® 488 anti-Tyrosine Hydroxylase a... -
Spark YG™ 570 anti-Tyrosine Hydroxylase
IHC staining of Spark YG™ 570 anti-Tyrosine Hydroxylase anti... IHC staining of Spark YG™ 570 anti-Tyrosine Hydroxylase anti...
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