- Clone
- A53-B/A2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Keratin 19, Keratin 19 type 1 cytoskeletal
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A53-BA2_AF647_Cytokeratin19_Antibody_IF_070114 A53-BA2_AF647_Cytokeratin19_Antibody_IF_070114](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A53-BA2_A647_Cytokeratin19_Antibody_ICC_080819.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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MCF-7 cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes at 37°C, permeabilized with 0.5% Triton X-100 buffer for 10 minutes at room temperature, blocked for 30 minutes at room temperature with 5% fetal bovine serum (FBS), then stained with 5 µg/mL anti-Cytokeratin 19 (clone A53-B/A2) Alexa Fluor® 647 (red). Nuclei were counterstained with DAPI (blue). Images were acquired with a 60X objective.
Cytokeratin 19, also known as keratin 19, is a type I intermediate filament protein with a molecular weight of approximately 40-44 kD. Cytokeratin 19 is a heterotetramer composed of two type I and two type II keratin subunits. Unlike other cytokeratins, cytokeratin 19 lacks a C-terminal non-helical extension. This cytokeratin is widely expressed in the periderm (transient superficial layer enveloping developing epidermis), muscle, intestine, bile duct, esophagus, stomach, and thymus. Cytokeratin 19 can be upregulated by vitamin A and is thought to play a critical role in embryogenesis. Cytokeratin 19 intereacts with the pinin protein and has been shown to be modified by phosphorylation (Ser10, Ser35).
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human mammary carcinoma cell line MCF-7
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemistry of paraffin-embedded sections, immunocytochemistry, and ELISA.
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Application References
(PubMed link indicates BioLegend citation) -
- Karsten U, et al. 1985. Eur. J. Cancer Clin. Oncol. 21:733.
- Nishikata T,et al. Anticancer Res. 33:2867. PubMed
- Product Citations
-
- RRID
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AB_2563651 (BioLegend Cat. No. 628506)
Antigen Details
- Structure
- Type I intermediate filament protein, contains three coiled-coil domains, lacks C-terminal non-helical extension found in other keratins, approximately 40-44 kD. Cytokeratin 19 is a heterotetramer composed of two type I and two type II keratin subunits.
- Distribution
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Expressed in the periderm (transient superficial layer enveloping developing epidermis), also expressed in muscle, intestine, bile duct, esophagus, stomach, and thymus. Upregulated by vitamin A.
- Function
- Intermediate filament protein involved with the cytoskeleton. May play a critical role in embryogenesis
- Interaction
- Interacts with pinin
- Modification
- Phosphorylation (Ser10, Ser35)
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Antigen References
-
1. Bader BL, et al. 1986. EMBO J. 5:1865.
2. Eckert RL. 1988. Proc. Natl. Acad. Sci. 85:1114.
3. Stasiak PC and Lane EB. 1987 Nucleic Acids Res. 15:10058. - Gene ID
- 3880 View all products for this Gene ID
- UniProt
- View information about Cytokeratin 19 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Cytokeratin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Cytokeratin 19 | A53-B/A2 | WB,ICC,IP,IHC-P,ELISA |
Alexa Fluor® 594 anti-Cytokeratin 19 | A53-B/A2 | ICC,IHC-P |
Alexa Fluor® 647 anti-Cytokeratin 19 | A53-B/A2 | ICC |
Alexa Fluor® 488 anti-Cytokeratin 19 | A53-B/A2 | ICC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Cytokeratin 19
Total cell lysate (15 µg protein) from MCF-7 were resolved ... MCF-7 cells were stained with anti-Cytokeratin 19 (clone A5... MCF-7 cells were stained with purified anti- Cytokeratin 19 ... -
Alexa Fluor® 594 anti-Cytokeratin 19
MCF7 cells were fixed with 1% paraformaldehyde (PFA), permea... Human paraffin-embedded colon was prepared with standard dep... -
Alexa Fluor® 647 anti-Cytokeratin 19
MCF-7 cells were fixed with 1% paraformaldehyde (PFA) for 10... -
Alexa Fluor® 488 anti-Cytokeratin 19
MCF-7 cells were fixed with 1% paraformaldehyde (PFA) for te...
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