- Clone
- A15102C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Lectin, galactoside-binding, soluble, 4 (LGALS4), GAL4, L36LBP, Galectin
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A15102C_A647_Galectin-4_Antibody_043020 A15102C_A647_Galectin-4_Antibody_043020](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A15102C_A647_Galectin-4_Antibody_043020.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human Galectin-4 (clone A15102C) Alexa Fluor® 647 (red) at 4°C overnight. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
Cat # | Size | Price | Save |
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397005 | 25 µg | ¥36,300 | |
397006 | 100 µg | ¥85,140 |
Galectin-4, also known as LGALS4, is a member of galectin family, a family of conserved animal lectins, which are characterized by their affinity for poly-N-acetyllactosamine-enriched glycoconjugates and sequence similarities in the carbohydrate recognition domain (CRD). Galectin-4 was initially identified in gastrointestinal mucosa including oral mucosa and esophagus. RNAse protection assays revealed that it is not expressed in the brain, kidney, skeletal muscle, heart, liver, and lung tissues. Similar to other galectins, galectin-4 does not have a signal peptide and is synthesized as a cytosolic protein, but it can be secreted. Galectin-4 controls intestinal inflammation and it binds to activated T cells at the CD3 region, which results in a potent inhibition of cell cycle progression and induction of antigen-induced cell death. Galectin-4 induces T cell death through the caspase-independent calcium-calpain signaling pathway. Galectin-4 is downregulated in colon cancer tissue and is absent in invasive carcinomas. Nevertheless, high levels in serum are detected in patients with colon and breast cancer. In contrast, it is considerably upregulated in pancreatic adenocarcinomas, hepatocellular carcinomas, and gastric cancer.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human Galectin-4
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- RRID
-
AB_2860969 (BioLegend Cat. No. 397005)
AB_2860970 (BioLegend Cat. No. 397006)
Antigen Details
- Structure
- Contains at least two distinct carbohydrate recognising domains (CRD).
- Distribution
-
It is expressed in oral mucosa, esophagus, gastrointestinal epithelium, and intestinal epithelial cell lines.
- Function
- Controls intestinal inflammation, intestinal epithelial wound healing, protein apical trafficking, and stabilizes adherens junctions and lipid rafts in the microvillus membrane.
- Interaction
- T cells and macrophages.
- Ligand/Receptor
- Galectin-4 binds to glycosphingolipids with 3-O-sulfated Gal residues and carcinoembryonic antigens.
- Cell Type
- Epithelial cells
- Biology Area
- Cancer Biomarkers, Immunology
- Antigen References
-
1. Rabinovich GA, et al. 2002. J Leuko Biol. 71:741.
2. Huflejt ME, Leffler H. 2004. Glycoconj J. 20:247.
3. Ideo H, et al. 2005. J Biol Chem. 6:4730-7.
4. Markova V, et al. 2006. Int J Mol Med. 18:65.
5. Paclik D, et al. 2008. Inflamm Bowel Dis. 14:1366.
6. Paclik D, et al. 2008. PLoS One 3(7):e2629.
7. Belo A, et al. 2013. PLoS One 8(6):e65957.
8. Houzelstein D, et al. 2013. J Histochem Cytochem. 61:348.
9. Viguier M, et al. 2014. Tissue Barriers 2:e29103.
10. Cai Z, et al. 2014. Cancer Sci. 105:1510. - Gene ID
- 3960 View all products for this Gene ID
- UniProt
- View information about Galectin-4 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Galectin-4 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human Galectin-4 | A15102C | IHC-P |
Alexa Fluor® 594 anti-human Galectin-4 | A15102C | IHC-P |
Alexa Fluor® 647 anti-human Galectin-4 | A15102C | IHC-P |
Alexa Fluor® 488 anti-human Galectin-4 | A15102C | IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human Galectin-4
Human paraffin-embedded colon cancer tissue slices were prep... -
Alexa Fluor® 594 anti-human Galectin-4
Human paraffin-embedded colon tissue slices were prepared wi... Human paraffin-embedded colon cancer tissue slices were prep... -
Alexa Fluor® 647 anti-human Galectin-4
Human paraffin-embedded colon tissue slices were prepared wi... -
Alexa Fluor® 488 anti-human Galectin-4
Human paraffin-embedded colon tissue slices were prepared wi...
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