- Clone
- LN3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Major Histocompatibility Class II, MHC class II
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![LN-3_Alx647_011108 LN-3_Alx647_011108](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/LN-3_Alx647_011108.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes stained with LN-3 Alexa Fluor® 647
The LN3 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- human PBL
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Red Laser (633 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: immunohistochemical staining1 of frozen sections and formalin-fixed paraffin-embedded sections1, and immunoprecipitation1.
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Application References
(PubMed link indicates BioLegend citation) -
- Marder RJ, et al. 1985. Lab. Invest. 52:497.
- Norton AJ and Isaacson PG. 1987. Am. J. Pathol. 128:225.
- Hua ZX, et al. 1998. Hum. Pathol. 29(12):1441.
- RRID
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AB_893570 (BioLegend Cat. No. 327012)
Antigen Details
- Structure
- Ig superfamily, MHC class II, heterodimeric transmembrane protein
- Distribution
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B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs
- Function
- Peptide presentation
- Ligand/Receptor
- CD3/TCR, CD4
- Cell Type
- Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
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1. Levacher M, et al. 1990. Clin. Exp. Immunol. 81:177.
2. Terstappen L, et al. 1990. J. Leuk. Biol. 48:138.
3. Edwards J, et al. 1985. J. Immunol. 137:490.
4. van Es A, et al. 1984. Transplantation 37:65.
5. O'Doherty U, et al. 1994. Immunology 82:487.
6. Thomas R, et al. 1994. J. Immunol. 153:4016.
7. Grouard G, et al. 1996. Nature 384:364. - Gene ID
- 3122 View all products for this Gene ID 3123 View all products for this Gene ID
- UniProt
- View information about HLA-DR on UniProt.org
Related FAQs
Other Formats
View All HLA-DR Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human HLA-DR | LN3 | FC,IHC,IP,SB |
Biotin anti-human HLA-DR | LN3 | FC,IHC |
FITC anti-human HLA-DR | LN3 | FC |
PE anti-human HLA-DR | LN3 | FC |
Alexa Fluor® 488 anti-human HLA-DR | LN3 | FC |
Alexa Fluor® 647 anti-human HLA-DR | LN3 | FC |
Alexa Fluor® 700 anti-human HLA-DR | LN3 | FC |
Pacific Blue™ anti-human HLA-DR | LN3 | FC |
PE/Cyanine7 anti-human HLA-DR | LN3 | FC |
PerCP/Cyanine5.5 anti-human HLA-DR | LN3 | FC |
APC anti-human HLA-DR | LN3 | FC |
APC/Fire™ 750 anti-human HLA-DR | LN3 | FC |
TotalSeq™-Bn1316 anti-human HLA-DR | LN3 | SB |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human HLA-DR
Human peripheral blood lymphocytes stained with purified LN3... SeqIF™ (sequential immunofluorescence) staining on COMET™ of... -
Biotin anti-human HLA-DR
Human peripheral blood lymphocytes stained with biotinylated... -
FITC anti-human HLA-DR
Human peripheral blood lymphocytes stained with LN-3 FITC -
PE anti-human HLA-DR
Human peripheral blood lymphocytes stained with LN-3 PE -
Alexa Fluor® 488 anti-human HLA-DR
Human peripheral blood lymphocytes stained with LN-3 Alexa F... -
Alexa Fluor® 647 anti-human HLA-DR
Human peripheral blood lymphocytes stained with LN-3 Alexa F... -
Alexa Fluor® 700 anti-human HLA-DR
Human peripheral blood lymphocytes stained with LN3 Alexa Fl... -
Pacific Blue™ anti-human HLA-DR
Human peripheral blood lymphocytes stained with LN3 Pacific ... -
PE/Cyanine7 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
PerCP/Cyanine5.5 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
APC anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
APC/Fire™ 750 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
TotalSeq™-Bn1316 anti-human HLA-DR
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