- Clone
- 695H1-9-9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Nonclassical MHC-Ib, H2-Qa2
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![695H1-9-9-Alx647_081406 695H1-9-9-Alx647_081406](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/695H1-9-9-Alx647_081406.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes stained with 695H1-9-9 Alexa Fluor® 647
Cat # | Size | Price | Save |
---|---|---|---|
121707 | 25 µg | ¥22,220 | |
121708 | 100 µg | ¥50,380 |
Mouse Qa-2 is a 40 kD GPI-linked surface protein. It is a member of nonclassical MHC class Ib molecule encoded by Q5, Q6, Q7, Q9, and Q10 genes of the H-2 complex. Qa-2 is a nonpolymorphic molecule primarily expressed on mouse T and B cells. The expression level of Qa-2 on mature T cells is higher than on B cells. Different mouse strains express Qa-2 at varying levels. Qa-2, in association with β2-microglobulin, binds to peptide and plays a role in antigen presentation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- C3H.SW mouse skin graft and splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation2.
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Application References
(PubMed link indicates BioLegend citation) -
- Sharrow SO, et al. 1989. J. Immunol. 142:3495.
- Patk E, et al. 1993. Microbiol. Immunol. 37:743.
- Product Citations
-
- RRID
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AB_571988 (BioLegend Cat. No. 121707)
AB_571989 (BioLegend Cat. No. 121708)
Antigen Details
- Structure
- GPI-linked protein, associates with β2-microglobulin, 40 kD
- Distribution
-
T and B cells, mature T cells expression higher than B cells
- Function
- Antigen presentation, promote immune response
- Ligand/Receptor
- Peptide
- Cell Type
- B cells, T cells
- Biology Area
- Immunology
- Molecular Family
- MHC Antigens
- Antigen References
-
1. Sharrow SO, et al. 1989. J. Immunol. 142:3495.
2. Mann DW, et al. 1987. J. Immunol. 138:240.
3. Mellor AL, et al.1985. Proc. Natl. Acad. Sci.USA. 82:5920.
4. Tabaczewski P, et al. 1997. J. Immunol. 159:2771.
5. Chiang EY and Stroynowski I. 2005. J. Immunol. 174:5367. - Gene ID
- 111458 View all products for this Gene ID
- UniProt
- View information about Qa-2 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Qa-2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Biotin anti-mouse Qa-2 | 695H1-9-9 | FC |
Alexa Fluor® 647 anti-mouse Qa-2 | 695H1-9-9 | FC |
FITC anti-mouse Qa-2 | 695H1-9-9 | FC |
Purified anti-mouse Qa-2 | 695H1-9-9 | FC |
APC anti-mouse Qa-2 | 695H1-9-9 | FC |
PE anti-mouse Qa-2 | 695H1-9-9 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse Qa-2
C57BL/6 mouse splenocytes stained with biotinylated 695H1-9-... -
Alexa Fluor® 647 anti-mouse Qa-2
C57BL/6 mouse splenocytes stained with 695H1-9-9 Alexa Fluor... -
FITC anti-mouse Qa-2
C57BL/6 mouse splenocytes were stained with Qa-2 (clone 695H... -
Purified anti-mouse Qa-2
C57BL/6 mouse splenocytes were stained with purified anti-mo... -
APC anti-mouse Qa-2
C57BL/6 mouse splenocytes were stained with APC anti-mouse Q... -
PE anti-mouse Qa-2
C57BL/6 mouse splenocytes were stained with anti-mouse Qa2 (...
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