- Clone
- SK7; SK3; HI30;
- Regulatory Status
- RUO
- Isotype
- Mouse IgG1, κ (all clones)
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![SK7-SK3-HI30_Cocktail_1_FC_081915 SK7-SK3-HI30_Cocktail_1_FC_081915](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/SK7-SK3-HI30_Cocktail_1_FC_081915.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with anti-human CD3 FITC/CD4 PE/CD45 PerCP cocktail. The top image shows SSC versus CD45+ profile ungated. The bottom image (CD3 versus CD4) image is gated on CD45+ lymphocytes as shown in the top image. -
CD3: SK7 antibody reacts with a combinatorial epitope of CD3ε, a 20 kD chain of the CD3/T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells, and some thymocytes.
CD4: SK3 antibody reacts with CD4, a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages.
CD45: HI30 antibody reacts with CD45, a 180-240 kD single chain type I membrane glycoprotein which is also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets.
Product Details
- Verified Reactivity
- Human
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- Preparation
- This reagent is a combination of FITC conjugated SK7, PE conjugated SK3, and PerCP conjugated HI30 at optimal concentration for three-color flow cytometric analysis.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood.
- Application Notes
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This cocktail has been optimized to be used in a lyse-wash and lyse-no wash assay.
Note:
Single color controls are required to perform instrument compensation and are not included in the kit. - RRID
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AB_2566176 (BioLegend Cat. No. 368302)
Antigen Details
- Distribution
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T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells, some thymocytes, and hematopoietic cells (not expressed in circulating erythrocytes or platelets).
- Cell Type
- Mesenchymal Stem Cells
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Antigen References
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1. Kan EA, et al. 1983. J. Immunol. 131:536.
2. Wood GS, et al. 1985. Am. J. Pathol. 120:371.
3. van Dongen JJ, et al. 1988. Blood 71:603.
4. Center D, et al. 1996. Immunol. Today 17:476.
5. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
6. Knapp W, et al. (1989) Leucocyte Typing IV. New York, NY: Oxford University Press.
7. Kishihara K, et al. 1993. Cell 74:143.
8. Esser M, et al. 2001. J. Virol. 75:6173. - Gene ID
- 916 View all products for this Gene ID 920 View all products for this Gene ID 5788 View all products for this Gene ID
Related Pages & Pathways
Pages
Related FAQs
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
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PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other Formats
View All Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Anti-human CD3 FITC/CD4 PE/CD45 PerCP Cocktail | SK7; SK3; HI30 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Anti-human CD3 FITC/CD4 PE/CD45 PerCP Cocktail
Human peripheral blood lymphocytes were stained with anti-hu...
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