- Clone
- Poly19251 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Protein Phosphatase 1 Regulatory Subunit 12A, Myosin Binding Subunit, Myosin Phosphatase, Target Subunit 1, Protein Phosphatase Myosin-Binding Subunit
- Previously
-
Covance Catalog# PRB-457C
- Isotype
- Rabbit Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![a-Purified_Myosin_Antibody_WB_032019 a-Purified_Myosin_Antibody_WB_032019](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Purified_Myosin_Antibody_WB_032019.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Total cell lysates (15 µg total protein) from HepG2, A431 HEL and Raw264.7 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with Purified anti-MyPt1 Antibody, clone Poly19251, at a 1:10000 dilution overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG Antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. -
Western Blot showing specificity of Myosin Phosphatase. Lane 1: Molecular weight marker; Lane 2: HeLa; Lane 3: BSA neg control.
Myosin phosphatase target subunit 1 (MYPT1) is a regulatory subunit of protein phosphatase 1. It regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The GTP-bound, active form of RhoA (GTP.RhoA), through its interaction with myosin-binding subunit (MBS) of myosin phosphatase, regulates the phosphorylation of myosin light chain (MLC). Rho inhibits myosin phosphatase through the action of Rho-kinase. Four splice-variant isoforms for the MYPT1 gene have been identified.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Reported Reactivity
- Dog
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- This antibody was generated against the N-terminal sequence (residues 1-38) of the large, non-catalytic subunit of myosin phosphatase.
- Preparation
- Serum
- Storage & Handling
- Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:10000 - 1:20000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
*Predicted MW = 115 kD
The non-catalytic subunit of myosin phosphate, the myosin binding subunit (MBS), is called M130 or MYPT1.
This product may contain other non-IgG subtypes. -
Application References
(PubMed link indicates BioLegend citation) -
- Muranyi A, et al. 1998. Biochem J. 330:225. (WB)
- Product Citations
-
- RRID
-
AB_2565464 (BioLegend Cat. No. 925101)
Antigen Details
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Gene ID
- 4659 View all products for this Gene ID
- UniProt
- View information about Myosin Phosphatase on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All Myosin Phosphatase Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Anti-Myosin Phosphatase | Poly19251 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Follow Us