Loading...

APC anti-mouse CD206 (MMR) Antibody

Pricing & Availability
Clone
C068C2 (See other available formats)
Regulatory Status
RUO
Other Names
MMR (macrophage mannose receptor), MR (mannose receptor), MRC1
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
C068C2_APC_1_082911
Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) PE, and then intracellularly stained with CD206 (clone C068C2) APC (top) or rat IgG2a, κ APC isotype control (bottom).
  • C068C2_APC_1_082911
    Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) PE, and then intracellularly stained with CD206 (clone C068C2) APC (top) or rat IgG2a, κ APC isotype control (bottom).
  • C068C2_APC_2_082911
Compare all formats See APC spectral data
Cat # Size Price Save
141707 25 µg ¥28,820
141708 100 µg ¥65,120
Description

CD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD206 (MMR)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Keller J, et al. 2012. Biochem Biophys Res Commun. 417:217. PubMed
  2. Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed
  3. Yang X, et al. 2015. PNAS. 112:2900. PubMed
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Wang C, et al. 2018. J Pharmacol Exp Ther. 365:72. PubMed
  2. Liu J, et al. 2017. Mucosal Immunol. 10.1038/mi.2016.139. PubMed
  3. Jiang Z, et al. 2021. J Clin Invest. 131: . PubMed
  4. Kiepura A, et al. 2021. Int J Mol Sci. 22:. PubMed
  5. Yokozeki Y, et al. 2021. Biomed Res Int. 2021:7988320. PubMed
  6. Liu C, et al. 2022. Regen Ther. 21:192. PubMed
  7. Miki H, et al. 2020. J Immunol. 204:1892. PubMed
  8. Zhou Y, et al. 2022. Br J Pharmacol. 179:1978. PubMed
  9. Liang X, et al. 2022. Cancer Commun (Lond). 42:205. PubMed
  10. Chang S, et al. 2022. Phytother Res. 36:1678. PubMed
  11. Tu J, et al. 2022. Cancer Sci. 113:2496. PubMed
  12. Wu J, et al. 2023. Bioact Mater. 20:259. PubMed
  13. Poh AR, et al. 2022. Sci Adv. 8:eabl7882. PubMed
  14. Poh AR, et al. 2022. Cell Rep. 41:111479. PubMed
  15. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  16. Lin J, et al. 2022. Eur J Histochem. 67: . PubMed
  17. Sun J, et al. 2023. Mediators Inflamm. 2022:1108569. PubMed
  18. Ye Z, et al. 2023. Front Med (Lausanne). 9:1075465. PubMed
  19. De Schepper S, et al. 2023. Nat Neurosci. 26:406. PubMed
  20. Kimura Y, et al. 2023. Front Immunol. 14:1070196. PubMed
  21. Liang P, et al. 2022. Prostate Cancer Prostatic Dis. 25:539. PubMed
  22. Gao T, et al. 2022. Cell Biochem Biophys. 80:579. PubMed
  23. Jia DJ, et al. 2022. Gut Microbes. 14:2145843. PubMed
  24. Song X, et al. 2022. Int J Mol Sci. 23:. PubMed
  25. Wu Y, et al. 2023. Adv Mater. 35:e2210787. PubMed
  26. Sheng Y, et al. 2023. Cancers (Basel). 15:. PubMed
  27. Wang Y, et al. 2023. Front Bioeng Biotechnol. 11:1128268. PubMed
  28. Kaur N, et al. 2023. Heliyon. 9:e14952. PubMed
  29. Lu Y, et al. 2023. J Transl Med. 21:339. PubMed
  30. Zhang Y, et al. 2023. NPJ Regen Med. 8:28. PubMed
  31. Hong M, et al. 2021. Int J Mol Sci. 22:. PubMed
  32. El-Hindi K, et al. 2022. Int J Mol Sci. 23:. PubMed
  33. He J, et al. 2022. Front Immunol. 12:760138. PubMed
  34. Pathak JL, et al. 2021. Front Cell Dev Biol. 628139:9. PubMed
  35. Jeong J, et al. 2017. Sci Rep. 7:39848. PubMed
  36. Poh AR, et al. 2017. Cancer Cell. 31:563. PubMed
  37. Liu C, et al. 2019. J Transl Med. 17:300. PubMed
  38. Zhang X, et al. 2017. J Cell Mol Med. 10.1111/jcmm.13329. PubMed
  39. Kurelac I, et al. 2019. Nat Commun. 10:903. PubMed
  40. Chang CF, et al. 2017. Neurobiol Dis. 103:54. PubMed
  41. Pilch Z, et al. 2018. Cancer Immunol Res. 6:930. PubMed
  42. Giordano C, et al. 2014. Hum Mol Genet. . PubMed
  43. Mylonas K, et al. 2015. Immunobiology. 220: 924-933. PubMed
  44. Cardona S, et al. 2015. ASN Neuro. 7: 1759091415608204. PubMed
  45. Tahiri H, et al. 2016. Sci Rep. 6:37391. PubMed
  46. Zhang J, et al. 2021. Front Cardiovasc Med. 8:737652. PubMed
  47. Chen L, et al. 2022. Mol Ther Oncolytics. 24:522. PubMed
  48. Liang S, et al. 2022. Acta Pharm Sin B. 12:2494. PubMed
  49. Adachi Y, et al. 2022. Nat Commun. 13:5117. PubMed
  50. Nawaz A, et al. 2022. Nat Commun. 13:7058. PubMed
  51. Zhang L, et al. 2022. Acta Pharm Sin B. 12:3427. PubMed
  52. Chen X, et al. 2021. Cell Death Differ. 28:1237. PubMed
  53. Trott DW, et al. 2021. Geroscience. 43:1331. PubMed
  54. Yuan Q, et al. 2021. Cell Reports. 34(5):108724. PubMed
  55. Young JA, et al. 2020. Journal of Neuroendocrinology. e12854.. PubMed
  56. Shang W, et al. 2019. J Diabetes Res. 2019:5641271. PubMed
  57. Wada N, et al. 2020. Mol Metab. 37:100988. PubMed
  58. Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed
  59. Motoyama S, et al. 2020. Cells. :9. PubMed
  60. Li S, et al. 2021. Ann Transl Med. 9:1644. PubMed
  61. Chen F, et al. 2022. Evid Based Complement Alternat Med. 2022:1240242. PubMed
  62. Helm M, et al. 2022. Life (Basel). 12:. PubMed
  63. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  64. Li Q, et al. 2021. Brain Behav Immun. 94:437. PubMed
  65. Kan C, et al. 2022. Bone Res. 10:62. PubMed
  66. Liao Y, et al. 2020. Mol Ther. 28:855. PubMed
  67. Hu M, et al. 2022. Can Respir J. 2022:5802938. PubMed
  68. Yi M, et al. 2021. J Hematol Oncol. 14:27. PubMed
  69. Qiu J, et al. 2016. Sci Rep. 6:39407. PubMed
  70. Lasky C, et al. 2015. Infect Immun . 83: 2627 - 2635. PubMed
  71. van Dierendonck XAMH, et al. 2020. Cell Reports. 30(6):1811-1822. PubMed
  72. Mariani SA, et al. 2019. Immunity. 50:1439. PubMed
  73. Yan J, et al. 2018. Nat Commun. 9:33. PubMed
  74. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  75. Xie Z, et al. 2021. Open Life Sci. 16:653. PubMed
  76. Wu L, et al. 2022. Front Cell Dev Biol. 9:684842. PubMed
  77. Chen X, et al. 2022. J Appl Oral Sci. 30:e20220316. PubMed
  78. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  79. Xiao Y, et al. 2022. Clin Transl Med. 12:e1071. PubMed
  80. Feng T, et al. 2022. Nat Commun. 13:5208. PubMed
  81. Kornstädt L, et al. 2021. Front Immunol. 11:607048. PubMed
  82. Chen L, et al. 2021. Cell Death Differ. 28:1880. PubMed
  83. Taguchi K, et al. 2015. J Urol. . PubMed
  84. Calvo B, et al. 2020. IBRO Rep. 8:36. PubMed
  85. Jin RM, et al. 2017. J Immunol. 198:352. PubMed
  86. Linde N, et al. 2018. Nat Commun. 9:21. PubMed
  87. Ayala TS, et al. 2019. Sci Rep. 9:11447. PubMed
  88. Taguchi K, et al. 2014. J Am Soc Nephrol. 25:1680. PubMed
  89. Rios-Doria J, et al. 2021. Front Oncol. 10:598477. PubMed
  90. Mallampalli RK, et al. 2021. Front Immunol. 12:735576. PubMed
  91. Xu M, et al. 2022. Genes Dis. 9:429. PubMed
  92. Ganesan R, et al. 2020. FASEB J. 34:15888. PubMed
  93. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  94. Xu Z, et al. 2020. Cancer Immunol Res. 1354:8. PubMed
  95. Xue N, et al. 2017. Sci Rep. 7:39011. PubMed
  96. Lee JY, et al. 2020. Nat Commun. 11:2358. PubMed
  97. Yoon J, et al. 2020. Front Cell Dev Biol. 0.520138889. PubMed
  98. Kumar MP, et al. 2018. Cell Rep. 25:1458. PubMed
  99. Wang Q, et al. 2018. Nat Commun. 9:559. PubMed
  100. Rhys HI, et al. 2018. EBioMedicine. 29:60. PubMed
  101. Sasaki T, et al. 2020. Cell Reports. 28(1):202-217.e7.. PubMed
  102. Moya-Pérez A, et al. 2015. PLoS One. 10: 0126976. PubMed
  103. De Simone G, et al. 2021. Immunity. :. PubMed
  104. Zhou X, et al. 2022. Front Pharmacol. 13:872188. PubMed
  105. Sun S, et al. 2021. Front Immunol. 12:777665. PubMed
  106. Rashid MH, et al. 2021. Oncol Rep. 45:1171. PubMed
  107. Zhang S, et al. 2021. J Inflamm Res. 14:4915. PubMed
  108. Sun M, et al. 2021. J Immunother Cancer. 9:. PubMed
  109. Fan Q, et al. 2021. Bioact Mater. 6:4014. PubMed
  110. Blriot C, et al. 2021. Immunity. :. PubMed
  111. Koikawa K, et al. 2021. Cell. 184:4753. PubMed
  112. Yamakawa D, et al. 2021. Cell Reports. 34(10):108817. PubMed
  113. Luo J et al. 2018. Immunity. 49(1):107-119 . PubMed
  114. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  115. Subramanian K, et al. 2019. Nat Microbiol. 4:62. PubMed
  116. Di Mitri D, et al. 2019. Cell Rep. 28:2156. PubMed
  117. Fujimoto N, et al. 2020. PLoS Biol. 18:e3000704. PubMed
  118. Remmerie A, et al. 2020. Immunity. 53:641. PubMed
  119. Quenum Zangbede FO, et al. 2018. J Neurosci. 38:6737. PubMed
  120. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  121. Ali S, et al. 2021. PLoS One. 16:e0246646. PubMed
  122. Trott DW, et al. 2021. J Physiol. 599:3973. PubMed
  123. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  124. Guo D, et al. 2022. Bone Res. 10:45. PubMed
  125. Yuan M, et al. 2022. Oxid Med Cell Longev. 2022:5479491. PubMed
  126. Li ZL, et al. 2021. Mol Ther Nucleic Acids. 25:502. PubMed
  127. Dong R, et al. 2021. Frontiers in Immunology. 12:620238. PubMed
  128. Zhang Y, et al. 2020. Mol Med Rep. 22:534. PubMed
  129. Kiyohara H, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:135. PubMed
  130. Settelmeier S, et al. 2020. PLoS One. 15:e0233261. PubMed
  131. He H, et al. 2012. Blood. 120:3152. PubMed
  132. Dai K, et al. 2015. J Leukoc Biol. 98: 1071 - 1080. PubMed
  133. Gao Z, et al. 2022. J Inflamm Res. 15:3337. PubMed
  134. Tomimatsu M, et al. 2022. Sci Rep. 12:16656. PubMed
  135. Rao L, et al. 2020. Nat Commun. 11:4909. PubMed
  136. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  137. Nam J, et al. 2021. Adv Ther (Weinh). 4:. PubMed
  138. Canedo T, et al. 2021. Neuropsychopharmacology. 46:2358. PubMed
  139. Zhang W, et al. 2021. Front Immunol. 12:768435. PubMed
  140. Roux C, et al. 2021. Antioxidants (Basel). 10:. PubMed
  141. Li K, et al. 2020. J Cell Mol Med. 24:13115. PubMed
  142. Huo M, et al. 2017. FASEB J. 10.1096/fj.201601030R. PubMed
  143. Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
  144. Lee SH, et al. 2018. J Exp Med. 215:357. PubMed
  145. Tsunekuni K, et al. 2020. J Clin Med. 9:00. PubMed
  146. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  147. Fang W, et al. 2021. J Exp Clin Cancer Res. 40:4. PubMed
  148. Tang PC, et al. 2022. Adv Sci (Weinh). 9:e2101235. PubMed
  149. Cui Y, et al. 2022. Front Oncol. 12:732860. PubMed
  150. Piirainen S, et al. 2021. Glia. 69:2459. PubMed
  151. Catrysse L, et al. 2021. Cell Rep. 36:109748. PubMed
  152. Zhu Y, et al. 2021. Int J Oral Sci. 13:25. PubMed
  153. Filtjens J, et al. 2021. Nat Commun. 12:2936. PubMed
  154. Siolas D, et al. 2021. Cell Reports. 36:109578. PubMed
  155. Canan C, et al. 2014. J Leukoc Biol. 96:473. PubMed
  156. Lee C, et al. 2020. Front Immunol. 11:77. PubMed
  157. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  158. Baardman J et al. 2018. Cell reports. 25(8):2044-2052 . PubMed
  159. Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed
  160. Durga Devi T, et al. 2017. Am J Pathol. 187:2659. PubMed
  161. Kurelac I, et al. 2019. J Clin Med. 1.85. PubMed
  162. Crowe J, et al. 2020. PLoS Pathog. 16:e1008391. PubMed
  163. Song HY, et al. 2021. Mol Ther. S1525-0016:00472-X. PubMed
  164. Luo ZW, et al. 2021. Int J Nanomedicine. 16:2949. PubMed
  165. Chithanathan K, et al. 2022. Biology (Basel). 11:. PubMed
  166. Ding T, et al. 2021. J Nanobiotechnology. 19:247. PubMed
  167. Andreata F, et al. 2021. STAR Protoc. 2:100831. PubMed
  168. Liu Y, et al. 2019. Am J Physiol Cell Physiol. 317:C762. PubMed
  169. Syed I et al. 2018. Cell metabolism. 27(2):419-427 . PubMed
  170. Nasti A, et al. 2017. Eur J Immunol. 47:2163. PubMed
RRID
AB_10896057 (BioLegend Cat. No. 141707)
AB_10900231 (BioLegend Cat. No. 141708)

Antigen Details

Structure
Type I transmembrane protein, 175 kD, C-type lectin superfamily
Distribution

Macrophages, dendritic cells, Langerhans cells, liver endothelial cells

Function
Pathogen recognition, endocytosis and phagocytosis, antigen presentation
Ligand/Receptor
Antigen containing mannose, fucose, or an N-acetyl glucosamine
Cell Type
Dendritic cells, Endothelial cells, Langerhans cells, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469.
3. Burgdorf S, et al. 2006. J. Immunol. 176:6770.
4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.

Gene ID
17533 View all products for this Gene ID
UniProt
View information about CD206 on UniProt.org

Related FAQs

Why is mouse CD206 stained intracellularly and not via surface staining?

Typically, mouse CD206 surface level is relatively low under normal conditions and so intracellular staining protocol is required to get better signal.

Go To Top Version: 3    Revision Date: 04/04/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account