- Clone
- 9H3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- C3b/C4b receptor, complement receptor type 1 (CR1)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![9H3_Biotin_CD35_Antibody_051523 9H3_Biotin_CD35_Antibody_051523](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/9H3_Biotin_CD35_Antibody_051523.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with anti-human CD20 APC and biotinylated CD35 (clone 9H3) (left) or biotinylated mouse IgG1, κ isotype control (right), followed by SAV-PE.
CD35 is a type I single chain of glycoprotein, also known as C3b/C4b receptor, Complement Receptor type 1 or CR1. Four molecular weight allotypes (160kD, 190kD, 220kD, and 250kD) have been described. CD35 is expressed on granulocytes, monocytes, B cells, erythrocytes, and follicular dendritic cells, as well as subsets of NK and T cells. CD35 binds complement C3b, C4b, or iC3, and iC4, and plays important roles in both innate and adoptive immune response via mediating phagocytosis by granulocytes and monocytes. CD35 has also been reported to inhibit T-cell proliferation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_2941509 (BioLegend Cat. No. 332408)
Antigen Details
- Distribution
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B cells, Dendritic cells, Erythrocytes, Granulocytes, Monocytes, NK cells, T cells
- Function
- Adhesion, mediate phagocytosis, inhibit T-cell proliferation
- Ligand/Receptor
- C3b, iC3, C4b, iC4
- Cell Type
- B cells, Dendritic cells, Erythrocytes, Granulocytes, Monocytes, NK cells, T cells
- Biology Area
- Cell Biology, Complement, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
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1. Vorup-Jensen et al. 2018. Front Immunol. 9:2716.
2. Ogembo et al. 2013. Cell Rep. 3(2):371-85. - Gene ID
- 1378 View all products for this Gene ID
- UniProt
- View information about CD35 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Other Formats
View All CD35 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD35 Antibody | 9H3 | FC |
FITC anti-human CD35 | 9H3 | FC |
PE anti-human CD35 Antibody | 9H3 | FC |
Biotin anti-human CD35 | 9H3 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD35 Antibody
Human peripheral blood lymphocytes were stained with purifie... -
FITC anti-human CD35
Human peripheral blood lymphocytes were stained with CD20 PE... -
PE anti-human CD35 Antibody
Human peripheral blood lymphocytes were stained with CD20 PE... -
Biotin anti-human CD35
Human peripheral blood lymphocytes were stained with anti-hu...
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