- Clone
- N152B/23 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Voltage-dependent anion-selective channel protein 1, porin 31HL, porin 31HM, plasmalemmal porin, outer mitochondrial membrane protein porin 1, PORIN, VDAC-1
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![N152Bslash23_Biotin_VDAC1_Antibody_1_090418 N152Bslash23_Biotin_VDAC1_Antibody_1_090418](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/N152Bslash23_Biotin_VDAC1_Antibody_1_090518_updated.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of Biotin anti-VDAC1 antibody (clone N152B/23). Lane 1: Molecular weight marker; Lane 2: 20 μg of human brain lysate; Lane 3: 20 μg of mouse brain lysate; Lane 4: 20 μg of rat brain lysate. The blot was incubated with 5 μg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Streptavidin (Cat. No. 405210). Enhanced chemiluminescence was used as the detection system. -
IHC staining of Biotin anti-VDAC1 antibody (clone N152B/23) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit was used (Multi-Species, component #5, DAB; Cat. No. 929901). Slides were counterstained with hematoxylin, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm -
IHC staining of Biotin anti-VDAC1 antibody (clone N152B/23) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit was used (Multi-Species, component #5, DAB; Cat. No. 929901). Slides were counterstained with hematoxylin, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm -
IHC staining of Biotin anti-VDAC1 antibody (clone N152B/23) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit was used (Multi-Species, component #5, DAB; Cat. No. 929901). Slides were counterstained with hematoxylin, according to the protocol provided. The image was captured with a 20X objective. Scale Bar: 50 µm
Cat # | Size | Price | Save |
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820704 | 100 µg |
This item is currently unavailable |
Voltage-dependent anion-selective channel protein 1 (VDAC1, also known as outer mitochondrial membrane protein porin 1, porin 31HL, and plasmalemmal porin) is a voltage-dependent anion channel and is the main calcium ion transport channel and plays a role in regulating the citric acid cycle and ROS generation. VDAC1 is implicated in cancer and neurodegenerative diseases through its ability to elevate ROS production.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a fusion protein corresponding to amino acids 1-283 (full-length) of human VDAC1.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).
- RRID
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AB_2750497 (BioLegend Cat. No. 820704)
Antigen Details
- Structure
- VDAC1 is a 283 amino acid protein with a molecular weight of 30 kD.
- Biology Area
- Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Mitochondrial Markers
- Gene ID
- 7416 View all products for this Gene ID
- UniProt
- View information about VDAC1 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Other Formats
View All VDAC1 Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-VDAC1 | N152B/23 | WB,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-VDAC1
Western blot of anti-VDAC1 antibody (clone N152B/23). Lane 1... IHC staining of anti-VDAC1 antibody (clone N152B/23) on form... IHC staining of anti-VDAC1 antibody (clone N152B/23) on form... IHC staining of anti-VDAC1 antibody (clone N152B/23) on form... IHC staining of anti-VDAC1 antibody (clone N152B/23) on form...
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