- Clone
- A18246A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-2Rα, Ly-43, p55, Tac
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A18246A_Brilliant-Violet-421_-CD25_Antibody_061523 A18246A_Brilliant-Violet-421_-CD25_Antibody_061523](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A18246A_Brilliant-Violet-421_-CD25_Antibody_061523-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with anti-mouse CD4 FITC and anti-mouse CD25 (clone A18246A) Brilliant Violet 421™ (left) or rat IgG2a, κ Brilliant Violet 421™ isotype control (right).
CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
- RRID
-
AB_3068095 (BioLegend Cat. No. 113705)
Antigen Details
- Structure
- Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
- Distribution
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Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells
- Function
- IL-2 receptor
- Ligand/Receptor
- IL-2
- Antigen References
-
- Taniguchi T, et al. 1993. Cell. 73:5-8.
- Waldmann TA. 1991. J Biol Chem. 266:2681-4.
- Read S, et al. 2000. J Exp Med. 192:295-302.
- Lowenthal JW, et al. 1985. J Immunol. 135:3988-94.
- Gene ID
- 16184 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
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It is lot-specific. On average it ranges between 2-4.
Other Formats
View All CD25 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD25 | A18246A | FC |
PE anti-mouse CD25 | A18246A | FC |
Brilliant Violet 421™ anti-mouse CD25 | A18246A | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD25
C57BL/6 mouse splenocytes were stained with anti-mouse CD4 a... -
PE anti-mouse CD25
C57BL/6 mouse splenocytes were stained with anti-mouse CD4 A... -
Brilliant Violet 421™ anti-mouse CD25
C57BL/6 mouse splenocytes were stained with anti-mouse CD4 F...
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