- Clone
- BV10A4H2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- FMS-like tyrosine kinase-3, FLT-3, STK-1, Flk-2
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![BV10A4H2_BV650_CD135_Antibody_070623 BV10A4H2_BV650_CD135_Antibody_070623](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/BV10A4H2_BV650_CD135_Antibody_070623.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human pre-B cell line REH was stained with anti-human CD135 (Flt-3/Flk-2) (clone BV10A4H2) Brilliant Violet 650™ (filled histogram) or mouse IgG1, κ Brilliant Violet 650™ isotype control (open histogram).
Cat # | Size | Price | Save |
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313329 | 25 tests | ¥50,380 | |
313330 | 100 tests | ¥95,700 |
CD135 is a 130-160 kD type III tyrosine kinase receptor expressed on CD34+ hematopoietic stem cells, myelomonocytic progenitors, primitive B cell progenitors, and thymocytes. CD135 is also expressed on malignant hematopoietic cells including AML, ALL and CML-BC. CD135, also known as FMS-like tyrosine kinase-3, FLT3, STK-1, and Flk-2, is a growth factor receptor that binds the FLT3 ligand to promote the growth and differentiation of primitive hematopoietic cells. The intracytoplasmic domain of CD135 is modified by phosphorylation and has been shown to interact with Grb2, SOCS1, VAV1, and Shc.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- BV-173 pro-B cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
Antigen Details
- Structure
- Member of the immunoglobulin supergene family, type 3 tyrosine kinase receptor; 130-160 kD
- Distribution
-
Expressed on CD34+ hematopoietic stem cells, myelomonocytic progenitors, primitive B cell progenitors, and thymocytes. Also expressed on AML, ALL and CML-BC tumor cells
- Function
- Tyrosine kinase growth factor receptor involved in the growth and differentiation of primitive hematopoietic cells
- Ligand/Receptor
- FLT3 ligand
- Cell Type
- B cells, Hematopoietic stem and progenitors, Leukemia, Thymocytes
- Biology Area
- Cell Biology, Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
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1. Rappold I, et al. 1997. Blood 90:111.
2. Rosnet O, et al. 1996. Acta Haematol. 95:218.
3. Rosnet O, et al. 1996. Leukemia 10:238.
4. Bertho JM, et al. 2000. Scand. J. Immunol. 52:53. - Gene ID
- 2322 View all products for this Gene ID
- UniProt
- View information about CD135 on UniProt.org
Other Formats
View All CD135 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD135 (Flt-3/Flk-2) | BV10A4H2 | FC |
PE anti-human CD135 (Flt-3/Flk-2) | BV10A4H2 | FC |
APC anti-human CD135 (Flt-3/Flk-2) | BV10A4H2 | FC |
PE/Cyanine5 anti-human CD135 (Flt-3/Flk-2) | BV10A4H2 | FC |
Biotin anti-human CD135 (Flt-3/Flk-2) | BV10A4H2 | FC |
PE/Cyanine7 anti-human CD135 (Flt-3/Flk-2) | BV10A4H2 | FC |
PerCP/Cyanine5.5 anti-human CD135 (Flt-3/Flk-2) | BV10A4H2 | FC |
TotalSeq™-A0351 anti-human CD135 (Flt-3/Flk-2) | BV10A4H2 | PG |
PE/Dazzle™ 594 anti-human CD135 (Flt-3/Flk-2) | BV10A4H2 | FC |
TotalSeq™-B0351 anti-human CD135 (Flt-3/Flk-2) | BV10A4H2 | PG |
TotalSeq™-C0351 anti-human CD135 (Flt-3/Flk-2) | BV10A4H2 | PG |
TotalSeq™-D0351 anti-human CD135 (Flt-3/Flk-2) | BV10A4H2 | PG |
Brilliant Violet 711™ anti-human CD135 (Flt-3/Flk-2) | BV10A4H2 | FC |
Brilliant Violet 650™ anti-human CD135 (Flt-3/Flk-2) | BV10A4H2 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD135 (Flt-3/Flk-2)
Human pre-B leukemia cell line REH stained with purified BV1... -
PE anti-human CD135 (Flt-3/Flk-2)
Human pre-B cell line REH stained with BV10A4H2 PE -
APC anti-human CD135 (Flt-3/Flk-2)
Human pre-B cell line REH stained with BV10A4H2 APC -
PE/Cyanine5 anti-human CD135 (Flt-3/Flk-2)
Human pre-B cell line REH stained with BV10A4H2 PE/Cyanine5 -
Biotin anti-human CD135 (Flt-3/Flk-2)
Human pre-B cell line REH stained with biotinylated BV10A4H2... -
PE/Cyanine7 anti-human CD135 (Flt-3/Flk-2)
Human acute lymphoblastic leukemia cell line REH was stained... -
PerCP/Cyanine5.5 anti-human CD135 (Flt-3/Flk-2)
Human pre-B cell line REH was stained with CD135 (clone BV10... -
TotalSeq™-A0351 anti-human CD135 (Flt-3/Flk-2)
-
PE/Dazzle™ 594 anti-human CD135 (Flt-3/Flk-2)
Human pre-B cell line REH was stained with CD135 (Flt-3/Flk-... -
TotalSeq™-B0351 anti-human CD135 (Flt-3/Flk-2)
-
TotalSeq™-C0351 anti-human CD135 (Flt-3/Flk-2)
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TotalSeq™-D0351 anti-human CD135 (Flt-3/Flk-2)
-
Brilliant Violet 711™ anti-human CD135 (Flt-3/Flk-2)
Human pre-B cell line REH was stained with anti-human CD135 ... -
Brilliant Violet 650™ anti-human CD135 (Flt-3/Flk-2)
Human pre-B cell line REH was stained with anti-human CD135 ...
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