- Clone
- 2G10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- RNA pol sigma 70, rpoD, sigma D, RNA Polymerase
- Isotype
- Mouse IgG2b
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![2G10_DBHRP_Ecoli_RNASigma70_Antibody_092216 2G10_DBHRP_Ecoli_RNASigma70_Antibody_092216](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/2G10_DBHRP_Ecoli_RNASigma70_Antibody_092216.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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15 µg of total protein extract from E. coli was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with purified or Direct-Blot™ HRP (DB-HRP) anti-E. coli RNA Sigma 70 (clone 2G10). Proteins were visualized using chemiluminescence detection.
RNA polymerase transcribes DNA to synthesize RNA. Prokaryotes contain a single RNA polymerase- core RNA polymerase. RNA polymerase sigma 70 (also known as RNA pol sigma 70 and rpoD) is a part of a family of sigma factor subunits that are required for specific promoter recognition and initiation. RNA pol sigma 70 is the major housekeeping sigma factor responsible for RNA polymerase recognition out of the majority of promoters necessary to transcribe bacterial genes which are expressed during expansion.
Product DetailsProduct Details
- Verified Reactivity
- E. coli
- Reported Reactivity
- Other species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant protein
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
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WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:4000. The optimal dilution should be determined by titration for each individual assay of interest.
25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution. - Application Notes
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Clone 2G10 is extremely useful for identifying the major sigma factor from a variety of bacteria. It does not react with Borellia burgdorferi. Clone 2G10 recognizes epitope 470-486.
Additional reported applications (for the relevant formats) include: functional assay and immunoprecipitation. -
Application References
(PubMed link indicates BioLegend citation) -
- Breyer MJ, et al. 1997. J. Bacterial 179:1404.
- Lesley SA, et al. 1989. J. Biol. Chem. 264:3794.
- Strickland MS, et al. 1988. Biochemistry 27:5755.
- Product Citations
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- RRID
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AB_2629596 (BioLegend Cat. No. 663205)
Antigen Details
- Structure
- Sigma 70 (σ70) is a 70 kD transcription factor of E. coli RNA polymerase.
- Biology Area
- Cell Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Gene ID
- 947567 View all products for this Gene ID
- UniProt
- View information about Sigma 70 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Sigma 70 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Direct-Blot™ HRP anti-E. coli RNA Sigma 70 | 2G10 | WB |
Purified anti-E. coli RNA Sigma 70 | 2G10 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Direct-Blot™ HRP anti-E. coli RNA Sigma 70
15 µg of total protein extract from E. coli was res... -
Purified anti-E. coli RNA Sigma 70
Whole cell extracts (15 µg protein) from BL21 E. coli...
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