LEGEND MAX™ Rat TNF-α ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
Rat TNF-α Pre-coated ELISA Kit
Ave. Rating
Submit a Review
Product Citations
publications
Rat_TNF-a_050611
  • Rat_TNF-a_050611
Cat # Size Price Save
438207 1 Pre-coated Plate ¥75,900
Description

TNF-a (Tumor Necrosis Factor a), also termed Cachectin, Cytotoxic Factor (CF), Hemorrhagic Factor, Macrophage Cytotoxic Factor (MCF), is a potent multifunctional cytokine which can exert regulatory, inflammatory and cytotoxic effects on a wide range of normal lymphoid, non-lymphoid cells and tumor cells. TNF-a is secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells, and many transformed cell lines. It exists as a soluble 17.5 kD protein and a 26 kD membrane-associated protein. The soluble form protein derives from the membrane form by proteolytic processing. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. TNF-a binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. It has been shown that TNF-a plays a critical role in many physiological and pathological conditions, including neurodegenerative diseases and cancer.

This kit is specifically designed to meet the increasing usage of rat models in biomedical studies due to larger sampling sizes, and the fact that rats have higher tolerance in chemotherapy, etc.

BioLegend’s Rat TNF-a LEGEND MAX™ ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with 96 well microtiter strip plates that are pre-coated with a capture antibody.

This ELISA kit is specifically designed for the accurate quantitation of Rat TNF-a from cell culture supernatant, serum, plasma or other biological fluids. This kit is analytically validated with ready-to-use reagents.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Anti-Rat TNF-α Pre-coated 96-well Strip Microplate
  • Rat TNF-α Dectection Antibody
  • Rat TNF-α Standard
  • Matrix A
  • Avidin-HRP D
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Rat
Application

ELISA

Application References

(PubMed link indicates BioLegend citation)
  1. Chi H, et al. 2013. Life Sci. 93:986. PubMed
Product Citations
  1. Tung TH, et al. 2022. Biomedicines. 10: . PubMed
  2. Hsieh CC, et al. 2023. J Ginseng Res. 47:210. PubMed
  3. Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
  4. Roberto Rodrigues Bicalho P, et al. 2020. Surg Res Pract. 2020:5738236. PubMed
  5. Zhang C, et al. 2022. Front Pharmacol. 12:790072. PubMed
  6. Jantaruk P, et al. 2017. PLoS One.. 10.1371/journal.pone.0183852. PubMed
  7. Yadav A, et al. 2021. J Neuroinflammation. 18:238. PubMed
  8. Lee SY, et al. 2022. Int J Mol Sci. 23:. PubMed
  9. Urkan M, et al. 2021. Ulus Travma Acil Cerrahi Derg. 27:284. PubMed
  10. Chia H, et al. 2013. Life Sci . 93:986. PubMed
  11. Kumari A, Dash D, Singh R 2017. Inflammopharmacology. 10.1007/s10787-017-0334-3. PubMed
  12. Chen CY, et al. 2021. Antioxidants (Basel). 10:. PubMed
  13. Huang KW, et al. 2020. Mol Ther Nucleic Acids. 1.042361111. PubMed
  14. Song J, et al. 2020. Mol Med Rep. 1.400694444. PubMed
  15. Yamanaka D, et al. 2017. Journal of Anesthesia. 10.1007/s00540-017-2374-7. PubMed
  16. Peng KT, et al. 2019. Clin Sci (Lond). 133:2171. PubMed
  17. Huang NC, et al. 2021. Int J Mol Sci. 22:. PubMed
Sensitivity
4.2 pg/mL
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
301289 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 2    Revision Date: 09/11/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account