LEGENDScreen™ Mouse PE Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
Mouse Cell Surface Marker Screening (PE) Kit, Mouse Antibody Panel (PE)
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Cat # Size Price Save
700009 1 kit ¥363,000
Description

BioLegend's LEGENDScreen™ Mouse Cell PE Kit contains 253 PE-conjugated monoclonal antibodies to cell surface markers as well as 11 mouse, rat, or hamster Ig isotype controls. All of the antibodies have been pre-titrated to optimal concentrations, arrayed on three 96-well plates, and lyophilized in a ready-to-use format. This kit can be used to screen cell lines and primary cells (splenocytes, bone marrow-derived cells, and cells isolated from tissues, etc.). There are blank wells on the plates for users to add their choice of markers. The end user can also choose to screen cells of interest directly or co-stain with other markers labeled with different fluorochromes. Positive "hits" from the screening can be quickly identified based on the plate map, and the catalog number and clone information can be obtained using the tables in the product manual. Analysis with individual fluorochrome-conjugated antibodies should be performed to confirm the screening results.

The LEGENDScreen™ Mouse Cell Screening (PE) Kit provides a convenient, easy-to-use, and powerful tool for immunology, stem cell, and cancer research.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Formulation
Lyophilized antibodies in 96-well plate
Storage & Handling
Store unopened kit components at 2 - 8°C. Do not use this kit beyond its expiration date. Once opened, reconstitute plates with distilled, deionized water. The reconstituted plates can be used immediately or sealed for storage at 2 - 8°C in the dark for up to one month. Keep the buffer(s) at 2 - 8°C and use within one month after opening.
Application

Cell Screening

Application Notes

Materials Provided in Kit:
3 Mouse Cell Screening Plates containing:
 · 253 PE-conjugated Monoclonal Antibodies
 · 11 Mouse, Rat, or Hamster Ig Isotype Controls
Cell Staining Buffer (Cat. No. 420201)
Fixation Buffer (Cat. No. 420801)
Plate Sealers (8 sheets)

Additional Product Notes

 Download the Excel Version of the Specificity/Clone List

RRID
AB_3097145 (BioLegend Cat. No. 700009)

Antigen Details

Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
NA

Related FAQs

If I don't have enough cells and use much less than 4 million/mL (~ 3 x 105 cells/well), will it still work?

This may work with lower numbers of total cells, but we recommend trying to keep higher concentrations of cells for faster analysis. Of course, how many cells are needed depends on the specific application. Successful staining has been done with 1 x 105 cells/well.

What are the guarantees regarding the lyophilized plate compared to the reconstituted plate?

Lyophilized product has a guaranteed shelf life of 6 months unopened. Reconstituted plates can be used or stored at 2 - 8°C sealed in the dark and used within a month. Be sure to properly seal the plates to prevent evaporation and shield the antibodies from light.

How should the kit be stored?

The kit should be stored at 2 - 8°C upon receipt. Once opened, the plates must be reconstituted immediately. Reconstituted plates can be used or stored at 2 - 8°C sealed in the dark and used within a month.

I have added my own antibody solution to the lyophilized product, will the lyophilized antibody work?

Yes, as long as the fluorophores on these antibodies are compatible and proper compensation has been applied during acquisition and analysis.

I am not going to use all the reconstituted antibody solution. Can I keep the left over for later or re-dry the solution in dark?

The antibody is in a one test per well format. There will not be any antibody left if the full test is used. Customers may decide to use less than the recommended volume per test, but this is not recommended and the performance is not guaranteed. Customers may also selectively transfer certain antibodies from the original plate to a new plate and use after reconstitution. If any antibody is not used after reconstitution, the plate can be sealed and store at 2 - 8°C for a month in the dark. Once reconstituted, re-drying is not recommended as this may result in a loss of signal.

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Can I use my existing institution discount for this new product?

Yes.

Can I use half or less of the plate and keep the rest for later?

Yes. Customers can use half of the plate or whatever specificities they are interested in. However, the whole plate should be reconstituted. The half plate of antibodies must be transferred to another empty plate for the staining. The remaining half must be sealed and stored at 2 - 8°C in the dark and used within a month.

Are these plates made under sterile conditions?

The plates are not sterile. You would handle them as you would handle most typical flow cytometry staining protocols.

How do I request a custom LEGENDScreen™ product with only my specificities of interest?

For more info, visit: biolegend.com/custom_solutions.

What is the level of variability from one experiment to the other?

If the protocol is followed closely, the variability should be minimal. The variability should be similar to single vial antibody staining.

Go To Top Version: 2    Revision Date: 09/21/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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