- Regulatory Status
- RUO
- Other Names
- Anti-Vitamin B7 Nanobeads, Anti-Vitamin H nanobeads, Anti-Biotinylated cell nanobeads
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_MojoSort_Biotin_Nanobeads_1_071724 A_MojoSort_Biotin_Nanobeads_1_071724](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/MojoSort_Biotin_Nanobeads_1_071724.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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A single cell suspension from human peripheral blood mononuclear cells (PBMCs) was prepared to isolate CD14+ cells using MojoSort™ anti-Biotin Nanobeads and biotinylated anti-human CD14 (clone 63D3) (Cat. No. 367105). Cells were stained with anti-human CD14 (clone HCD14) PE (Cat. No. 325605) and anti-human CD3 (clone UCHT1) FITC (Cat. No. 300452). The analysis was pre-gated with anti-human CD45 (clone HI30) APC/Cyanine7 (Cat. No. 304014) staining. Dead cells were excluded using Helix NP™ Blue (Cat. No. 425305). -
Starting with human whole blood, CD8+ cells were isolated using MojoSort™ anti-Biotin Nanobeads and biotinylated anti-human CD8 (clone SK1) (Cat. No. 344720). Cells were stained with anti-human CD8 (clone RPA-T8) PE (Cat. No. 301004) and anti-human CD3 (clone UCHT1) APC (Cat. No. 300412). The analysis was pre-gated with anti-human CD45 (clone HI30) APC/Cyanine7 (Cat. No. 304014) staining. Dead cells were excluded using Helix NP™ Blue (Cat. No. 425305). -
A single cell suspension from human peripheral blood mononuclear cells (PBMCs) was positively selected for CD19+ cells using the MojoSort™ anti-Biotin Nanobeads and biotinylated anti-human CD19 (Cat. No. 302204). Cells were stained for flow cytometry with anti-human CD19 (clone HIB19) APC (Cat. No. 302212) and anti-human CD3 (clone UCHT1) APC (Cat. No.300412). Dead cells were excluded using Helix NP™ Blue (Cat. No. 425305).
Cat # | Size | Price | Save |
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480032 | 100 µL | ¥35,200 | |
480034 | 1 mL | ¥102,080 |
MojoSort™ anti-Biotin Nanobeads can be used for positive or negative selection of targeted cells with biotin-conjugated antibodies. For positive selection, the magnetically labeled fraction is retained by the use of a magnetic separator. For negative selection, the untouched cells are collected by decanting the liquid in a clean tube. Either positive or negative selection cells can be used for downstream applications such as functional assays, gene expression, phenotypic characterization, etc.
A complete list of BioLegend’s biotin-conjugated antibodies can be found here.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
Kit Contents
- Kit Contents
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For Cat. No. 480032:
- 1 vial containing 100 µL of anti-Biotin Nanobeads
For Cat. No. 480034:- 1 vial containing 1 mL of anti-Biotin Nanobeads
Product Details
- Verified Reactivity
- Species independent, all species
- Formulation
- Particle: Aqueous solution containing BSA and 0.05% sodium azide.
- Preparation
- Anti-Biotin conjugated particles: protein-coated magnetic nanobeads.
- Storage & Handling
- Anti-Biotin Nanobeads should be stored undiluted between 2°C and 8°C
- Application
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Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
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The concentration of anti-Biotin Nanobeads to be used should be optimized by end users.
- Application Notes
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The MojoSort™ anti-Biotin Nanobeads are designed for the positive or negative isolation of cells when used with biotin-conjugated antibodies or similar reagents.
Nanobead dilution to use in column: To be optimized by the end user
A complete list of BioLegend’s biotin-conjugated antibodies can be found here.
Antigen Details
- Gene ID
- NA
- UniProt
- View information about Biotin on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Is there a way to detach your magnetic particles from the cell surface?
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MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.
MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.
- What is the size of your magnetic particles?
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MojoSort Nanobeads: the average diameter is approximately 130 nm.
MojoSort Microbeads: the average diameter is approximately 1 μm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
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MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- What antibodies are present in the depletion cocktails provided for isolation kits?
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Please contact our technical service team for further assistance.
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