- Clone
- 1D3/CD19 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- B4, CVID3
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1D3_CD19_PacificBlue_CD19_Antibody_090222 1D3_CD19_PacificBlue_CD19_Antibody_090222](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/1D3_CD19_PacificBlue_CD19_Antibody_090222.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with anti-mouse CD3 FITC and anti-mouse CD19 (clone 1D3/CD19) Pacific Blue™ (left) or mouse IgG2a, κ Pacific Blue™ isotype control (right).
Cat # | Size | Price | Save |
---|---|---|---|
152415 | 25 µg | ¥20,680 | |
152416 | 100 µg | ¥47,300 |
CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Transfected k562 cells expressing the extracellular domain of murine CD19.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: blocking1,2 and immunoprecipitation1.
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Application References
(PubMed link indicates BioLegend citation) -
- Krop I, et al. 1996. Eur. J. Immunol. 26:238. (FC, Blocking, IP)
- Abraham P, et al. 2014. Clin. Exp. Immunol. 175:181. (Blocking)
- RRID
-
AB_2927869 (BioLegend Cat. No. 152415)
AB_2927869 (BioLegend Cat. No. 152416)
Antigen Details
- Structure
- Ig superfamily, associates with CD21 and CD81, 95 kD.
- Distribution
-
Pro-B cells to mature B cells (during development), follicular dendritic cells.
- Function
- Modulates B cell activation and differentiation.
- Ligand/Receptor
- CD21, CD81, Leu-13.
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437. - Gene ID
- 12478 View all products for this Gene ID
- UniProt
- View information about CD19 on UniProt.org
Related FAQs
Other Formats
View All CD19 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD19 | 1D3/CD19 | FC,Block,IP |
FITC anti-mouse CD19 | 1D3/CD19 | FC |
PerCP/Cyanine5.5 anti-mouse CD19 | 1D3/CD19 | FC |
PE anti-mouse CD19 | 1D3/CD19 | FC |
APC anti-mouse CD19 | 1D3/CD19 | FC |
APC/Cyanine7 anti-mouse CD19 | 1D3/CD19 | FC |
Alexa Fluor® 700 anti-mouse CD19 | 1D3/CD19 | FC |
Pacific Blue™ anti-mouse CD19 | 1D3/CD19 | FC |
PE/Cyanine7 anti-mouse CD19 | 1D3/CD19 | FC |
Biotin anti-mouse CD19 | 1D3/CD19 | FC |
Brilliant Violet 421™ anti-mouse CD19 | 1D3/CD19 | FC |
Brilliant Violet 510™ anti-mouse CD19 | 1D3/CD19 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD19
C57BL/6 mouse splenocytes were stained with purified CD19 (c... -
FITC anti-mouse CD19
C57BL/6 mouse splenocytes were stained with CD19 FITC (clone... -
PerCP/Cyanine5.5 anti-mouse CD19
C57BL/6 mouse splenocytes were stained with CD19 (clone 1D3/... -
PE anti-mouse CD19
C57BL/6 mouse splenocytes were stained with CD19 (clone 1D3/... -
APC anti-mouse CD19
C57BL/6 mouse splenocytes were stained with CD19 APC (clone ... -
APC/Cyanine7 anti-mouse CD19
C57BL/6 mouse splenocytes were stained with anti-mouse CD3 F... -
Alexa Fluor® 700 anti-mouse CD19
C57BL/6 mouse splenocytes were stained with anti-mouse CD3 F... -
Pacific Blue™ anti-mouse CD19
C57BL/6 mouse splenocytes were stained with anti-mouse CD3 F... -
PE/Cyanine7 anti-mouse CD19
C57BL/6 mouse splenocytes were stained with anti-mouse CD3 F... -
Biotin anti-mouse CD19
C57BL/6 mouse splenocytes were stained with biotinylated ant... -
Brilliant Violet 421™ anti-mouse CD19
C57BL/6 mouse splenocytes were stained with anti-mouse CD3 (... -
Brilliant Violet 510™ anti-mouse CD19
C57BL/6 mouse splenocytes were stained with anti-mouse CD3 (...
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