PE anti-human CD148 Antibody

Pricing & Availability
Clone
A3 (See other available formats)
Regulatory Status
RUO
Other Names
HPTP-eta, DEP-1, p260
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A3_PE_102108.jpg
Human peripheral blood lymphocytes stained with A3 PE
  • A3_PE_102108.jpg
    Human peripheral blood lymphocytes stained with A3 PE
See PE spectral data
Cat # Size Price Save
328708 100 tests ¥58,960
Description

CD148, a receptor-like protein tyrosine phosphatase also known as human protein tyrosine phosphatase-eta (HPTP-eta) or density-enhanced protein tyrosine phosphatase-1 (DEP-1), is involved in signal transduction in leucocytes and is thought to contribute to mechanisms of cellular differentiation. In lymphoid organs, CD148 was found to be widely expressed on B and T cells, granulocytes, macrophages, certain dendritic cells as well as mature thymocytes. The cellular level of CD148 was increased after in vitro activation of peripheral blood leucocytes. CD148 as a leucocyte activation marker and may be involved in the regulation of T cell activation. Leucocytes expressing CD148 are significantly upregulated in inflamed tissues and that a subset of these cells co-expresses the activation marker CD25. In non-lymphoid tissues, CD148 was found to be present on many epithelial cell types with glandular and/or endocrine differentiation as well as on fibrocytes, melanocytes and Schwann cells. Among non-hematopoietic cells, CD148 is expressed by characteristic types of epithelial and non-epithelial cells. Downregulation of CD148 might promote dedifferentiation and autonomous growth of such cells in malignant tumors.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
PHA-stimulated human PBMC
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Tangye SG. 1998. J. Immunol. 161:3249. (FC)
  2. Tangye SG. 1998. J. Immunol. 161:3803.
  3. Tangye SG. 1998. J. Exp. Med. 188:1691.
  4. Autschbach F. 1999. Tissue Antigens 54:485.
  5. Lin J and Weiss A. 2003. J. Cell Biol. 162:673. (IF)
  6. Whiteford, JR., et al. 2011. Mol Cell Biol. 22:3609. PubMed.
Product Citations
  1. Chappert P, et al. 2022. Immunity. 55:1872. PubMed
RRID
AB_2174826 (BioLegend Cat. No. 328708)

Antigen Details

Distribution

Monocytes, T cells, B cells, NK cells, epithelial cells, fibrocytes, melanocytes and Schwann cells

Cell Type
Monocytes, T cells, B cells, NK cells, Epithelial cells, Fibroblasts, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
5796 View all products for this Gene ID
UniProt
View information about CD148 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 02/04/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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