Analyte Specific Reagent. Analytical and performance characteristics are not established.
- Clone
- M-A251
- Workshop
- IV A053
- Other Names
- IL-2 receptor α chain, Low affinity IL-2R, IL-2Rα chain
- Isotype
- Mouse IgG1, κ
![M-A251_PE_CD25_ASR_061224 M-A251_PE_CD25_ASR_061224](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/M-A251_PE_CD25_ASR_061224.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Typical results from human peripheral blood lymphocytes stained either with M-A251 PE used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
CD25 is a 55 kD type I transmembrane glycoprotein also known as low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses. CD25 associates with IL-2 receptor β (CD122) and common γ (CD132) chains to form a high affinity IL-2R complex.
Product DetailsProduct Details
- Reactivity
- Human
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 0.2% (w/v) BSA (origin USA), and a stabilizer.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 100 µg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
- Disclaimer
-
WARNINGS AND PRECAUTIONS
- Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
- This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
- All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
- Do not use this reagent beyond the expiration date stated on the label.
- Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be an indication of possible deterioration.
- Avoid prolonged exposure of the reagent or stained cells to light.
Antigen Details
- Antigen References
-
1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Barclay N, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press Inc.
4. Taniguchi T and Minami Y. et al. 1993. Cell 73:5.
5. Waldmann T. 1991. J. Biol. Chem. 266:2681.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC/Cyanine7 anti-human CD25
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
Purified anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
PE anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
FITC anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
PE/Cyanine7 anti-human CD25
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
APC anti-human CD25
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
PerCP/Cyanine5.5 anti-human CD25
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
Brilliant Violet 421™ anti-human CD25
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
Alexa Fluor® 488 anti-human CD25
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
Alexa Fluor® 700 anti-human CD25
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
Brilliant Violet 510™ anti-human CD25
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
PE/Dazzle™ 594 anti-human CD25
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
Biotin anti-human CD25
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
Alexa Fluor® 647 anti-human CD25
PHA-stimulated (3 days) human peripheral blood lymphocytes w... Confocal image of human lymph node sample acquired using the... -
Pacific Blue™ anti-human CD25
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
PerCP anti-human CD25
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
APC/Fire™ 750 anti-human CD25
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
Brilliant Violet 711™ anti-human CD25
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
Brilliant Violet 785™ anti-human CD25
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
Brilliant Violet 605™ anti-human CD25
PHA-stimulated (3 days) human peripheral blood lymphocytes w... Human peripheral blood lymphocytes were stained with CD4 APC... -
KIRAVIA Blue 520™ anti-human CD25
PHA-stimulated (3 days) human peripheral blood lymphocytes w... Human peripheral blood lymphocytes were stained with CD4 APC... -
PE/Fire™ 700 anti-human CD25
PHA-stimulated (3 days) human peripheral blood lymphocytes w... Human peripheral blood lymphocytes were stained with anti-hu... -
APC/Fire™ 810 anti-human CD25
PHA-stimulated (3 days) human peripheral blood lymphocytes w... Human peripheral blood lymphocytes were stained with CD4 Bri... -
Spark NIR™ 685 anti-human CD25 Antibody
Human peripheral blood lymphocytes were stained with CD4 FIT... -
Spark YG™ 581 anti-human CD25
PHA-stimulated (3 days) human peripheral blood lymphocytes w... Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 640 anti-human CD25 Antibody
PHA-stimulated (3 days) human peripheral blood lymphocytes w... Human peripheral blood lymphocytes were stained with CD4 Bri... -
PE anti-human CD25
Typical results from human peripheral blood lymphocytes stai... -
PerCP/Cyanine5.5 anti-human CD25
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 750 anti-human CD25
Typical results from human peripheral blood lymphocytes stai... -
PE/Cyanine7 anti-human CD25
Typical results from human peripheral blood lymphocytes stai... -
APC anti-human CD25
Typical results from human peripheral blood lymphocytes stai... -
PE/Cyanine5 anti-human CD25
Human peripheral blood lymphocytes were stained with anti-hu... -
FITC anti-human CD25
Typical results from human peripheral blood lymphocytes stai... -
Spark Red™ 718 anti-human CD25
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP PE anti-human CD25
Typical results from human peripheral blood lymphocytes stai... -
GMP PE/Cyanine7 anti-human CD25
Typical results from human peripheral blood lymphocytes stai... -
PerCP/Fire™ 780 anti-human CD25
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 744 anti-human CD25
PHA-stimulated (3 days) human peripheral blood mononuclear c... Human peripheral blood mononuclear cells were stained with a... -
PerCP/Fire™ 806 anti-human CD25
Human peripheral blood lymphocytes were stained with anti-hu... -
Brilliant Violet 650™ anti-human CD25
Human peripheral blood lymphocytes were stained with anti-hu... -
Brilliant Violet 750™ anti-human CD25
Human peripheral blood lymphocytes were stained with anti-hu... Human peripheral blood lymphocytes were stained with anti-hu...
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