- Clone
- OX-101 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- gp42, IAP, neurophilin
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![OX-101_PE_110308.jpg OX-101_PE_110308.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/OX-101_PE_110308.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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LOU rat splenocytes stained with OX-101 PE
CD47, also known as Integrin-Associated Protein (IAP), is a membrane protein of about 50 kD with an IgV-like extracelluluar domain, a five membrane-spanning segment, and a short terminal cytoplasmic region. It is widely expressed on many cell types and often associated with beta 3 integrins. The significance of this molecule is recently drawing increasing attention. It has been reported that CD47 functions as a self marker. Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages. By binding to SIRPα, CD47 controls hemostatic innate immune functions, such as phagocytosis and cell trafficking.
Product DetailsProduct Details
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
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Application References
(PubMed link indicates BioLegend citation) -
- DeVries HE, et al. 2002. J. Immunol. 168:5832.
- RRID
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AB_2074948 (BioLegend Cat. No. 204108)
Antigen Details
- Structure
- 50 kD plasma membrane protein of immunoglobulin supergene family, with an extracellular IgV-like domain of 120 amino acids, a five membrane spanning segment of 152 amino acids, and a short cytoplasmic tail of 30 amino acids.
- Distribution
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Broad tissue distribution, including haemopoietic cells, epithelial and endothelial cells, fibroblasts, erythrocytes and other tissue cells.
- Function
- Adhesion molecules, immune regulation
- Interaction
- Associate with β3 integrins
- Ligand/Receptor
- SIRPα (CD172α, signal regulatory protein alpha), thrombospondin
- Cell Type
- Epithelial cells, Endothelial cells, Fibroblasts, Erythrocytes
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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- Vernon-Wilson, et al. 2000. Eur. J. Immunol. 30:2130.
- Brown E, et al. 1990. J. Cell. Biol. 111:2785.
- Mawby WJ, et al. 1994. Biochem. J. 304:525.
- Gene ID
- 16423 View all products for this Gene ID
- UniProt
- View information about CD47 on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All CD47 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-rat CD47 | OX-101 | FC |
PE/Cyanine7 anti-rat CD47 | OX-101 | FC |
APC anti-rat CD47 | OX-101 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-rat CD47
LOU rat splenocytes stained with OX-101 PE -
PE/Cyanine7 anti-rat CD47
LOU rat splenocytes were surface stained with CD47 (clone OX... -
APC anti-rat CD47
LOU rat splenocytes were surface stained with CD47 (clone OX...
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