- Clone
- A19016A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Signal Transducer and Activator Of Transcription 4, SLEB11, Signal Transducer and Activator of Transcription 4 Variant 3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A19016A_PE_Stat4_Antibody_1_013123 A19016A_PE_Stat4_Antibody_1_013123](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A19016A_PE_Stat4_Antibody_1_013123.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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NK-92 cells untreated (negative control, open histogram), or treated with hIL-2 (100 ng/mL) and hIFNα2 (100 ng/mL) (positive control, solid histogram) were fixed and permeabilized using True Phos™ Perm Buffer (Cat No. 425401) and intracellularly stained with PE anti-Stat4 Phospho (Tyr 693) (clone A19016A), or PE Mouse IgG1, κ isotype control (open histogram, dashed line)(representative for both untreated and treated NK-92 cells) (Cat No. 981804). -
PBMC pre-treated overnight with cell activation cocktail (1:2500)(Cat. No. 423301), were stimulated with (positive control, solid histogram) or without (negative control, open histogram) hIL-2 (100 ng/mL) and IFNα2 (100 ng/mL) for 30 min. Cells were then fixed and permeabilized using True Phos™ Perm Buffer (Cat No. 425401) and intracellularly stained with PE anti-Stat4 Phospho (Tyr693) (clone A19016A), or PE Mouse IgG1, κ isotype control (open histogram, dashed line) (representative for both untreated and treated PBMC) (Cat No. 981804).
Cat # | Size | Price | Save |
---|---|---|---|
941205 | 25 tests | ¥28,600 | |
941206 | 100 tests | ¥69,300 |
The signal transducer and activator of transcription (STAT) family of transcription factors are DNA-binding proteins required for transcriptional regulation of several cellular and physiological processes in response to cytokine and growth factor stimulation. Inactive STAT proteins are present as cytosolic monomers. C-terminal tyrosine phosphorylation by upstream Janus kinases results in STAT dimerization, translocation to the nucleus, and increased affinity for response elements found in the regulatory promoter of STAT target genes.
In contrast to most other members of the STAT family, STAT4 expression is restricted to hematopoietic cells and responds to fewer cytokines, such as IL-12 and IL-2. STAT4, which requires phosphorylation at tyrosine 693 for activation, plays a pivotal role in the differentiation and proliferation of Th1 and Th17 cells, both of which are crucial effectors of chronic autoimmune disorders. In natural killer cells, STAT4 is required for normal development, maturation, and activation. Single nucleotide polymorphisms of STAT4 are implicated in the pathogenesis of systemic lupus erythematosis and rheumatoid arthritis, and are linked with increased risk of hepatocellular carcinoma.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide corresponding to human STAT4 phosphorylated at tyrosine 693
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
This antibody is predicted to recognize mouse STAT4 phosphorylated at tyrosine 694 and rat STAT4 phosphorylated at tyrosine 693 due to complete sequence homology between the immunizing peptide and the respective murine STAT4 orthologs.
- RRID
-
AB_2936724 (BioLegend Cat. No. 941205)
AB_2936724 (BioLegend Cat. No. 941206)
Antigen Details
- Structure
- STAT4 is a 748 amino acid protein with predicted molecular weight of 86 kD.
- Distribution
-
Hematopoietic cells/Nucleocytoplasmic localization
- Function
- Transcription factor, cell signaling
- Biology Area
- Cell Biology, Signal Transduction, Transcription Factors
- Antigen References
-
Trinchieri G, et al. 2003. Nat. Rev. Immunol. 3:133.
Remmers EF, et al. 2007. N. Engl J. Med. 357:977.
Mukasa R, et al. 2010. Immunity. 32:616
Rapp M, et al. 2017. Sci. Immunol. 2:3296. - Gene ID
- 6775 View all products for this Gene ID
- UniProt
- View information about STAT4 Phospho on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All STAT4 Phospho Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-STAT4 Phospho (Tyr693) Antibody | A19016A | WB,ICFC |
Alexa Fluor® 647 anti-STAT4 Phospho (Tyr693) | A19016A | ICFC |
PE anti-STAT4 Phospho (Tyr693) | A19016A | ICFC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-STAT4 Phospho (Tyr693) Antibody
Whole cell extracts (15 µg protein) from serum-starved NK-92... NK-92 cells untreated (negative control, open histogram), or... PBMC pre-treated overnight with cell activator cocktail (1:2... -
Alexa Fluor® 647 anti-STAT4 Phospho (Tyr693)
NK-92 cells untreated (negative control, open histogram), or... PBMC pre-treated overnight with cell activation cocktail (1:... -
PE anti-STAT4 Phospho (Tyr693)
NK-92 cells untreated (negative control, open histogram), or... PBMC pre-treated overnight with cell activation cocktail (1:...
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