- Clone
- 3A20.2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SAv
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![3A20.2_PE_Streptavidin_Antibody_030116 3A20.2_PE_Streptavidin_Antibody_030116](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/3A20.2_PE_Streptavidin_Antibody_030116.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 thymocytes were sequentially stained with CD4 Biotin, Streptavidin purified, anti-Streptavidin (clone 3A20.2) PE antibody (filled histogram), or mouse IgG2b, κ PE isotype control (open histogram).
Cat # | Size | Price | Save |
---|---|---|---|
410503 | 25 µg | ¥17,600 | |
410504 | 100 µg | ¥47,080 |
Streptavidin is a 52.8 kD tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used in a wide range of applications including flow cytometry, molecular biology, and bionanotechnology.
Product DetailsProduct Details
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Streptavidin
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
The 3A20.2 purified antibody is useful as a third step reagent to amplify dim or low signals in many applications. This antibody does not cross-react with avidin.
- Product Citations
-
- RRID
-
AB_2571914 (BioLegend Cat. No. 410503)
AB_2571915 (BioLegend Cat. No. 410504)
Antigen Details
- Structure
- 52.8 kD tetrameric protein.
- Function
- Streptavidin binds to biotin with high affinity.
- Ligand/Receptor
- Biotin
- Antigen References
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1. Dundas CM, et al. 2013. Appl. Microbiol. Biotechnol. 97:9343.
2. Zhao X, et al. 2013. J. Anal Methods Chem. 58:1093.
3. Kaplan DL, et al. 1999. Biomol. Eng. 16:135.
4. Wilbur DS, et al. 1999. Biomol. Eng. 16:113.
5. Sano T, et al. 1998. J. Chromatogr. B. Biomed. Sci. Appl. 715:85.
- Gene ID
- NA
- UniProt
- View information about Streptavidin on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All Streptavidin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Streptavidin Antibody | 3A20.2 | FC |
PE anti-Streptavidin Antibody | 3A20.2 | FC |
Brilliant Violet 421™ anti-Streptavidin Antibody | 3A20.2 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Streptavidin Antibody
C57BL/6 thymocytes were sequentially stained with CD4 Biotin... -
PE anti-Streptavidin Antibody
C57BL/6 thymocytes were sequentially stained with CD4 Biotin... -
Brilliant Violet 421™ anti-Streptavidin Antibody
C57BL/6 thymocytes were sequentially stained with biotinylat...
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