- Clone
- L363 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![L363_PECy7_alpha-GalCer-CD1d_Antibody_101218 L363_PECy7_alpha-GalCer-CD1d_Antibody_101218](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/L363_PECy7_alpha-GalCer-CD1d_Antibody_101218.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Murine CD1d1 transfected RMA-s cells were treated with (filled histogram) or without (open histogram) a-GalCer overnight, then stained with a-GalCer:CD1d complex (clone L363) PE/Cyanine7.
Cat # | Size | Price | Save |
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140515 | 25 µg | ¥34,980 | |
140516 | 100 µg | ¥81,400 |
The L363 antibody reacts with α-GalCer:mouse CD1d complex. Glycolipid α-galactosylceramide (α-GalCer) is a potent activator of human and murine iNKT cells and is widely used as a model antigen for the studies of iNKT-mediated immunity. The glycolipid binds to CD1d and forms a complex which is subsequently recognized by iNKT cells, a subset of T cells expressing invariant TCR α chain Vα14, analogous to the peptide-MHC complex recognition by TCR α/ß T cells.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- α-GalCer:mCD1d1 complexes coupled to Mycobacterium tuberculosis PPD
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: Surface and intracellular immunofluorescence2 and block a-GalCer:mCD1d1 complex induced iNKT activation1.
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
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Application References
(PubMed link indicates BioLegend citation) -
- Yu KO, et al. 2007. J. Immunol. Methods 323:11. (Block)
- Im JS, et al. 2009. Immunity 30:888. (IF)
- RRID
-
AB_2783103 (BioLegend Cat. No. 140515)
AB_2783104 (BioLegend Cat. No. 140516)
Antigen Details
- Distribution
-
NKT cells
- Cell Type
- B cells, Dendritic cells, NKT cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, MHC Antigens, TCRs
- Antigen References
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1. Arrenberg P, et al. 2010. P. Natl. Acad. Sci. USA 107:10984.
2. Mattarollo SR, et al. 2010. J. Immunol. 184:5663.
3. Wang J, et al. 2010. P. Natl. Acad. Sci. USA 107:1535.
4. Nieuwenhuis EE, et al. 2009. J. Clin. Invest. 119:1241.
5. Sullivan BA and Kronenberg M. 2005. J. Clin. Invest. 115:2328. - Gene ID
- 12479 View all products for this Gene ID
- UniProt
- View information about alpha-GalCer:CD1d complex on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All α-GalCer:CD1d complex Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-mouse α-GalCer:CD1d complex | L363 | FC |
PE/Dazzle™ 594 anti-mouse α-GalCer:CD1d complex | L363 | FC |
Biotin anti-mouse α-GalCer:CD1d complex | L363 | FC |
APC anti-mouse α-GalCer:CD1d complex | L363 | FC |
PerCP/Cyanine5.5 anti-mouse α-GalCer:CD1d complex | L363 | FC |
PE/Cyanine7 anti-mouse α-GalCer:CD1d complex | L363 | FC |
Ultra-LEAF™ Purified anti-mouse α-GalCer:CD1d complex | L363 | FC,Block,ICC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-mouse α-GalCer:CD1d complex
Murine CD1d1 transfected RMA-s cells were treated with (fill... -
PE/Dazzle™ 594 anti-mouse α-GalCer:CD1d complex
Murine CD1d1 transfected RMA-s cells were treated with (fill... -
Biotin anti-mouse α-GalCer:CD1d complex
Murine CD1d1 transfected RMA-s cells were treated with (fill... -
APC anti-mouse α-GalCer:CD1d complex
Murine CD1d1 transfected RMA-s cells were treated with (fill... -
PerCP/Cyanine5.5 anti-mouse α-GalCer:CD1d complex
Murine CD1d transfected RMA-s cells were treated with (fille... -
PE/Cyanine7 anti-mouse α-GalCer:CD1d complex
Murine CD1d1 transfected RMA-s cells were treated with (fill... -
Ultra-LEAF™ Purified anti-mouse α-GalCer:CD1d complex
Murine CD1d1 transfected RMA-s cells loaded with (yellow) or...
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