- Clone
- 6B4C88 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-15RA, IL-15Rα, IL-15 Receptor α subunity
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![6B4C88_PEDazzle594_CD215_Antibody_032522 6B4C88_PEDazzle594_CD215_Antibody_032522](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/6B4C88_PEDazzle594_CD215_Antibody_032522.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Mouse bone marrow derived dendritic cells were stimulated overnight with LPS. Cells were stained with anti-mouse CD215 (IL-15Rα) (clone 6B4C88) PE/Dazzle™ 594 (filled histogram) or with rat IgG2a, κ PE/Dazzle™ 594 isotype control (open histogram).
Cat # | Size | Price | Save |
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153511 | 25 µg | ¥38,720 | |
153512 | 100 µg | ¥84,040 |
IL-15 receptor α (IL-15Rα) is a cytokine receptor with the structural homology to IL-2Rα (CD25). Both α receptors contain a group of β sandwich protein called sushi domains. Similar to IL-2Rα, IL-15Rα is able to form a heterotrimer with their β chain (CD122) and common γ chain (γc, CD132). But unlike IL-2Rα subunit, IL-15Rα itself binds to IL-15 with equivalent high affinity compared with the heterotrimeric complex. IL-15Rα is expressed as soluble or transmembrane forms by variety cells, such as activated monocytes, dendritic cells, CD8+ memory T cells, NK or NKT cells. IL-15Rα by itself forms stable complexes with IL-15 on cell surface and presents IL-15 in trans to neighboring cells. IL-15, like IL-2, is a pleiotropic four-helix bundle cytokine and plays a key role in promoting T and B cell survival and proliferation, preferentially in memory CD8+ T cell proliferation, and NK/NKT cell development.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CD215 recombinant protein (33-205 a.a.)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- RRID
-
AB_2922479 (BioLegend Cat. No. 153511)
AB_2922479 (BioLegend Cat. No. 153512)
Antigen Details
- Structure
- IL-15 receptor α (IL-15Rα) is a cytokine receptor with the structural homology of IL-2Rα (CD25). Both α receptors contain a group of β sandwich protein called sushi domains.
- Distribution
-
It is found on a variety of subsets including dendritic cells, activated monocytes, memory CD8 T cells and NK/NKT cells.
- Function
- IL-15Rα is involved in T and B cell proliferation and development, preferentially for memory CD8+ T cell proliferation, and NK/NKT cell development
- Ligand/Receptor
- IL-15
- Cell Type
- Dendritic cells, Monocytes, NK cells, NKT cells, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Giri JG, et al. 1995. EMBO J. 14:3654.
2. Giron-Michel J, et al. 2005. Blood. 106:2302.
3. Lorenzen I, et al. 2005. J. Biol. Chem. 281:6642.
4. Sato N, et al. 2007. Proc. Natl. Acad. Sci. USA. 104:588.
5. Anderson BG, Quinn LS. 2016. Endocrinology. 157:1315 - Gene ID
- 16169 View all products for this Gene ID
- UniProt
- View information about CD215 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CD215 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD215 (IL-15Rα) | 6B4C88 | FC |
PE anti-mouse CD215 (IL-15Rα) | 6B4C88 | FC |
APC anti-mouse CD215 (IL-15Rα) | 6B4C88 | FC |
TotalSeq™-A1019 anti-mouse CD215 (IL-15Rα) | 6B4C88 | PG |
PE/Cyanine7 anti-mouse CD215 (IL-15Rα) | 6B4C88 | FC |
PE/Dazzle™ 594 anti-mouse CD215 (IL-15Rα) | 6B4C88 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD215 (IL-15Rα)
Mouse bone marrow derived dendritic cells stimulated overnig... -
PE anti-mouse CD215 (IL-15Rα)
Mouse bone marrow derived dendritic cells stimulated overnig... -
APC anti-mouse CD215 (IL-15Rα)
Mouse bone marrow derived dendritic cells stimulated overnig... -
TotalSeq™-A1019 anti-mouse CD215 (IL-15Rα)
-
PE/Cyanine7 anti-mouse CD215 (IL-15Rα)
Mouse bone marrow derived dendritic cells were stimulated ov... -
PE/Dazzle™ 594 anti-mouse CD215 (IL-15Rα)
Mouse bone marrow derived dendritic cells were stimulated ov...
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