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PerCP/Cyanine5.5 anti-EGFR Antibody

Pricing & Availability
Clone
A19002A (See other available formats)
Regulatory Status
RUO
Other Names
Epidermal Growth Factor Receptor, Receptor Tyrosine-Protein Kinase ErbB-1, Erb-B2 Receptor Tyrosine Kinase 1, ERBB1, NISBD2, PIG61, MENA, Proto-oncogene c-ErbB-1
Isotype
Mouse IgG1, κ
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Product Citations
publications
A19002A_-PerCP-Cyanine55_EGFR_ICFC_070924
A-431 cells (positive cell line, filled histogram) and MOLT-4 cells (low-expressing cell line, open histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with PerCP/Cyanine5.5 anti-EGFR (clone A19002A), or PerCP/Cyanine5.5 mouse IgG1, κ isotype control (open histogram, dashed line) (Cat No. 400150).
  • A19002A_-PerCP-Cyanine55_EGFR_ICFC_070924
    A-431 cells (positive cell line, filled histogram) and MOLT-4 cells (low-expressing cell line, open histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with PerCP/Cyanine5.5 anti-EGFR (clone A19002A), or PerCP/Cyanine5.5 mouse IgG1, κ isotype control (open histogram, dashed line) (Cat No. 400150).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Save
933905 25 tests ¥37,400
933906 100 tests ¥93,500
Description

Epidermal Growth Factor Receptor (EGFR) is a receptor tyrosine kinase that links extracellular mitogenic ligand binding to complex downstream signaling cascades. Initial ligand binding results in receptor oligomerization and autophosphorylation of multiple tyrosine residues within cytosolic domains of the the protein. These phosphorylation events stabilize the EGFR kinase activation loop and lead to the recruitment of adaptor proteins and other downstream effectors. EGFR stimulation leads to activation of RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCγ-PKC and STAT signaling cascades, and constitutive activation of the receptor promotes tumorigenesis in multiple cancers.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide from human EGFR
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

For use of this antibody (clone A19002A) in intracellular flow cytometry (ICFC), fixation/permeabilization using Cyto-Fast™ Perm Buffer (Cat. No. 426803) is recommended.

 

Antigen Details

Structure
EGFR is a 1,210 amino acid protein with a predicted molecular weight of 134 kD; the processed form is 1,186 amino acids in length.
Distribution

Plasma membrane/Ubiquitously expressed

Function
EGFR signaling, cell signaling
Cell Type
Endothelial cells, Epithelial cells
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family
CD Molecules, Growth Factors
Antigen References
  1. Hunter T and Cooper JA. 1981. Cell. 24:741.
  2. Gill GN and Lazar CS. 1981. Nature. 293:305.
  3. Reynolds FH, et al. 1981. Nature. 292:259
  4. Zhou M, et al. 2013. Cancer Res. 23:7056-67
Gene ID
1956 View all products for this Gene ID
UniProt
View information about EGFR on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All EGFR Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-EGFR A19002A WB,ICC,IHC-P,ICFC,IP
Alexa Fluor® 647 anti-EGFR A19002A ICFC,IHC-P
Go To Top Version: 1    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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