- Clone
- Ber-ACT35 (ACT35) (See other available formats)
- Regulatory Status
- RUO
- Other Names
- ACT35 antigen, tumor necrosis factor receptor superfamily member 4 (TNFRSF4)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![BER-ACT35_PerCPCyanine5dot5_CD134_Antibody_030419_updated.png BER-ACT35_PerCPCyanine5dot5_CD134_Antibody_030419_updated.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/BER-ACT35_PerCPCyanine5dot5_CD134_Antibody_030419_updated.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were activated for 3 days with PHA, and then stained with CD3 APC and CD134 (OX-40) (clone Ber-ACT35) PerCP/Cyanine5.5 (left) or Mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
Cat # | Size | Price | Save |
---|---|---|---|
350009 | 25 tests | ¥38,720 | |
350010 | 100 tests | ¥84,040 |
CD134, also known as OX40 and TNFRSF4, is a 50 kD type I transmembrane glycoprotein. It is a member of the TNF receptor family. OX40 is expressed on activated T lymphocytes including Th1, Th2, Th17, and Treg cells. The interaction of OX40 with OX40L results in B cell proliferation and antibody secretion, regulation of primary T cell expansion, and T cell survival. OX40 influences the size of the T cell memory pool and regulation of CD4+ T cell tolerance.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- HTLV 1-transformed HUT 102 cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Excitation Laser
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Blue Laser (488 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, immunohistochemical staining2,3of paraffin embedded7 and frozen tissue sections, ELISA4, and functional assay5. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact our custom solutions team).
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
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Application References
(PubMed link indicates BioLegend citation) -
- Latza U, et al. 1994. Eur. J. Immunol. 24:677. (WB, IP)
- Durkop H, et al. 1995. Brit. J. Haematol. 91:927. (IHC)
- Durkop H, et al. 1997. Brit. J. Haematol. 98:863. (IHC)
- Willett B, et al. 2007. J. Virol. 81:9665. (ELISA)
- Li M and Zhang Y. et al. 2005. Cell. Mol. Immunol. 2:467. (FA)
- Gloviczki ML, et al. 2012. Clin. J. Am. Soc. Nephrol. 8:546. PubMed
- Domingos PL, et al. 2012. An. Bras. Dermatol. 87:851. (IHC)
- Product Citations
-
- RRID
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AB_10720986 (BioLegend Cat. No. 350009)
AB_10719224 (BioLegend Cat. No. 350010)
Antigen Details
- Structure
- 50 kD, TNF receptor family
- Distribution
-
Activated T cells
- Function
- Receptor for OX40 ligand, co-stimulatory signal for T cell proliferation and survival.
- Ligand/Receptor
- OX40L (CD252)
- Cell Type
- T cells, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Smith CA, et al. 1994. Cell. 76:959.
2. Chen AL, et al. 1999. Immunity. 11:689.
3. Croft M. 2010. Annu. Rev. Immunol. 28:57.
4. Withers DR, et al. 2009. J. Immunol. 183:5079.
5. Klinger M, et al. 2009. J. Immunol. 182:4581. - Gene ID
- 7293 View all products for this Gene ID
- UniProt
- View information about CD134 on UniProt.org
Related FAQs
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
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PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other Formats
View All CD134 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-human CD134 (OX40)
Human peripheral blood lymphocytes were activated for 3 days... -
Purified anti-human CD134 (OX40)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... Formalin-fixed paraffin-embedded human tonsil treated with a... -
PE anti-human CD134 (OX40)
3-day PHA-activated human peripheral blood lymphocytes were ... -
APC anti-human CD134 (OX40)
Human peripheral blood lymphocytes were activated for 3 days... -
PerCP/Cyanine5.5 anti-human CD134 (OX40)
Human peripheral blood lymphocytes were activated for 3 days... -
PE/Cyanine7 anti-human CD134 (OX40)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
Brilliant Violet 421™ anti-human CD134 (OX40)
PHA-stimulated (3-days) human peripheral blood lymphocytes w... -
Purified anti-human CD134 (OX40) (Maxpar® Ready)
Human PBMCs were incubated for 3 days in media alone (top) o... -
Alexa Fluor® 647 anti-human CD134 (OX40)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
PE/Dazzle™ 594 anti-human CD134 (OX40)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
APC/Cyanine7 anti-human CD134 (OX40)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
Biotin anti-human CD134 (OX40)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
Brilliant Violet 510™ anti-human CD134 (OX40)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
Brilliant Violet 605™ anti-human CD134 (OX40)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
APC/Fire™ 750 anti-human CD134 (OX40)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
Brilliant Violet 711™ anti-human CD134 (OX40)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
TotalSeq™-A0158 anti-human CD134 (OX40)
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TotalSeq™-C0158 anti-human CD134 (OX40)
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TotalSeq™-B0158 anti-human CD134 (OX40)
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TotalSeq™-D0158 anti-human CD134 (OX40)
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PE/Cyanine5 anti-human CD134 (OX40)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
PE/Fire™ 810 anti-human CD134 (OX40)
Human peripheral blood lymphocytes were activated for 3 days... -
PE anti-human CD134
Typical result from PHA-stimulated (3 days) human peripheral... -
PE/Fire™ 744 anti-human CD134 (OX40)
PHA-stimulated (three days) human peripheral blood lymphocyt...
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