- Clone
- HD30 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- DEC-205
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![HD30_PerCPCyanine55_CD205_Antibody_101818 HD30_PerCPCyanine55_CD205_Antibody_101818](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/HD30_PerCPCyanine55_CD205_Antibody_101818.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Monocyte-derived dendritic cells were stained with CD205 (DEC-205, clone HD30) PerCP/Cyanine5.5 (filled histogram), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
Cat # | Size | Price | Save |
---|---|---|---|
342209 | 25 tests | ¥45,320 | |
342210 | 100 tests | ¥90,420 |
CD205 is a 210 kD C-type lectin transmembrane protein, known as DEC-205. It belongs to macrophage mannose receptor family and is found at high levels on dendritic cells and thymic epithelial cells. Unlike murine CD205, human CD205 is also expressed at low levels on T- and B-cells, NK cells and monocytes. CD205, serves as an endocytic receptor, functions in antigen uptake/processing and clearance of apoptotic cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a purified extracellular domain of human CD205-IgG1 Fc fusion protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of fixed, frozen sections4 and Western blotting4.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
-
Application References
(PubMed link indicates BioLegend citation) -
- Guo M, et al. 2000. Human Immunology 61:729.
- Cho H, et al. 2007. Physiol Genomics doi:10.1152/physiolgenomics.00051.2006
- Gurer C, et al. 2008. Blood 112:1231. PubMed
- Park CG, et al. 2012. J. Immunol. Methods 377:15. (IHC, WB)
- RRID
-
AB_2234763 (BioLegend Cat. No. 342209)
AB_2137644 (BioLegend Cat. No. 342210)
Antigen Details
- Structure
- Macrophage mannose receptor family, C-type lectin, 210 kD
- Distribution
-
High levels on dendritic cells and thymic epithelial cells, low levels on lymphocytes and monocytes
- Function
- Antigen uptake and process
- Cell Type
- Dendritic cells, Epithelial cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Guo M, et al. 2000. Human Immunology 61:729.
2. Kato M, et al. 2006. Int. Immunol.
3. Kato M, et al. 2003. J. Biol. Chem. 278(36):34035.
4. Small M and G. Kraal. 2003. Int. Immunol. 15(2):197.
5. Mahnke K, et al. 2000. J. Cell Biol. 151(3):673. - Gene ID
- 4065 View all products for this Gene ID
- UniProt
- View information about CD205 on UniProt.org
Related FAQs
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
-
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other Formats
View All CD205 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD205 (DEC-205) | HD30 | FC,IHC-P,WB |
PE anti-human CD205 (DEC-205) | HD30 | FC |
Alexa Fluor® 647 anti-human CD205 (DEC-205) | HD30 | FC |
APC anti-human CD205 (DEC-205) | HD30 | FC |
PerCP/Cyanine5.5 anti-human CD205 (DEC-205) | HD30 | FC |
TotalSeq™-A0814 anti-human CD205 (DEC-205) | HD30 | PG |
TotalSeq™-C0814 anti-human CD205 (DEC-205) | HD30 | PG |
TotalSeq™-B0814 anti-human CD205 (DEC-205) Antibody | HD30 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human CD205 (DEC-205)
Monocyte-derived dendritic cells stained with HD30 PE -
PE anti-human CD205 (DEC-205)
Monocyte-derived dendritic cells stained with HD30 PE -
Alexa Fluor® 647 anti-human CD205 (DEC-205)
Monocyte-derived dendritic cells stained with HD30 Alexa Flu... -
APC anti-human CD205 (DEC-205)
Human monocyte-derived dendritic cells stained with HD30 APC -
PerCP/Cyanine5.5 anti-human CD205 (DEC-205)
Monocyte-derived dendritic cells were stained with CD205 (DE... -
TotalSeq™-A0814 anti-human CD205 (DEC-205)
-
TotalSeq™-C0814 anti-human CD205 (DEC-205)
-
TotalSeq™-B0814 anti-human CD205 (DEC-205) Antibody
Follow Us