- Clone
- 12A5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Folate Receptor 4, FBP, FR δ
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![12A5_PerCPCyanine55_FR4_Antibody_1_101323 12A5_PerCPCyanine55_FR4_Antibody_1_101323](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/12A5_PerCPCyanine55_FR4_Antibody_1_101323.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with CD3 FITC and FR4 (Folate Receptor 4, clone 12A5) PerCP/Cyanine5.5 (left) or rat IgG2b, κ PerCP/Cyanine5.5 isotype control (right).
Cat # | Size | Price | Save |
---|---|---|---|
125017 | 25 µg | ¥34,980 | |
125018 | 100 µg | ¥81,400 |
Folate receptor 4 (FR4) is a surface receptor for folic acid (Vitamin B9).It was recently reported that FR4 is highly and constitutively expressed on mouse CD4+ CD25+ natural regulatory T cells (Treg).The high expression of this surface marker on Treg can be used in combination with CD4 and CD25 to distinguish Treg from other types of T cells.Since FR4 is a surface marker,this antibody may be used in combination with anti-CD4 and/or CD25 to sort living T reg cells for functional studies.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Excitation Laser
-
Blue Laser (488 nm)
- Additional Product Notes
-
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
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Application References
(PubMed link indicates BioLegend citation) -
- Yamaguchi T et al. 1007:Immunity 27(1)145
- RRID
-
AB_2721723 (BioLegend Cat. No. 125017)
AB_2721724 (BioLegend Cat. No. 125018)
Antigen Details
- Structure
- 28 kd protein consisting of 244 amino acids. Heavily glycosylated
- Distribution
-
Expressed on mouse thymocytes and spleen cells. Highly expressed on natural regulatory (Treg) cells
- Function
- Mediate calcium-independent homophlic cell-cell adhesion
- Ligand/Receptor
- Folic acid and its derivative
- Cell Type
- Tregs
- Biology Area
- Immunology, Innate Immunity
- Antigen References
-
1. Yamaguchi T et al 2007. Immunity 27:145
2. Spiegelstein O et al. 2000 Gene 258:117 - Gene ID
- 64931 View all products for this Gene ID
- UniProt
- View information about FR4 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
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PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other Formats
View All FR4 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
FITC anti-mouse FR4 (Folate Receptor 4) | 12A5 | FC |
PE anti-mouse FR4 (Folate Receptor 4) | 12A5 | FC |
Alexa Fluor® 647 anti-mouse FR4 (Folate Receptor 4) | 12A5 | FC |
PE/Cyanine7 anti-mouse FR4 (Folate Receptor 4) | 12A5 | FC |
PerCP/Cyanine5.5 anti-mouse FR4 (Folate Receptor 4) | 12A5 | FC |
PE/Dazzle™ 594 anti-mouse FR4 (Folate Receptor 4) | 12A5 | FC |
APC/Fire™ 750 anti-mouse FR4 (Folate Receptor 4) | 12A5 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-mouse FR4 (Folate Receptor 4)
C57BL/6 mouse splenocytes stained with CD3 (145-2C11) APC an... -
PE anti-mouse FR4 (Folate Receptor 4)
C57BL/6 mouse splenocytes stained with Cd3 (145-2C11) APC an... -
Alexa Fluor® 647 anti-mouse FR4 (Folate Receptor 4)
C57BL/6 mouse splenocytes stained with CD3 (145-2C11) PE and... -
PE/Cyanine7 anti-mouse FR4 (Folate Receptor 4)
C57BL/6 mouse splenocytes stained with CD3 (145-2C11) APC an... -
PerCP/Cyanine5.5 anti-mouse FR4 (Folate Receptor 4)
C57BL/6 mouse splenocytes were stained with CD3 FITC and FR4... -
PE/Dazzle™ 594 anti-mouse FR4 (Folate Receptor 4)
C57BL/6 mouse splenocytes were stained with CD3 APC and FR4 ... -
APC/Fire™ 750 anti-mouse FR4 (Folate Receptor 4)
C57BL/6 mouse splenocytes were stained with CD3 FITC and FR4...
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