- Clone
- W18272D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W18272D_PERCP-CYA55_IFN-G_Antibody_122823 W18272D_PERCP-CYA55_IFN-G_Antibody_122823](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W18272D_PERCP-CYA55_IFN-G_Antibody_122823.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were treated with (left) or without (right) Cell Activation Cocktail (with Brefeldin A) (Cat. Nos. 423303 and 423304) for 5 hours. Cells were then surface stained with anti-mouse CD3 (clone 145-2C11) FITC, then fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat: 426803) and intracellularly stained with anti-mouse IFN-γ (clone W18272D) PerCP/Cyanine5.5.
Cat # | Size | Price | Save |
---|---|---|---|
163509 | 25 µg | ¥24,200 | |
163510 | 100 µg | ¥73,700 |
IFN-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Excitation Laser
-
Blue Laser (488 nm)
- RRID
-
AB_3097440 (BioLegend Cat. No. 163509)
AB_3097440 (BioLegend Cat. No. 163510)
Antigen Details
- Structure
- Cytokine; dimer; 40-80 kD (Mammalian)
- Distribution
-
Tregs
- Function
- Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
- Ligand/Receptor
- Receptors: IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
- Antigen References
-
1. Zaidi et al. 2011, Clin Cancer Res,17(19):6118-242. Castro et al. 2018, Front Immunol, 9:8473. Alspach et al. 2019, Cold Spring Harb Perspect Biol, 11(3):a028480
- Gene ID
- 15978 View all products for this Gene ID
- UniProt
- View information about IFN-gamma on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
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PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other Formats
View All IFN-γ Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-mouse IFN-γ Antibody | W18272D | ICFC |
APC/Fire™ 750 anti-mouse IFN-γ | W18272D | ICFC |
PE/Cyanine7 anti-mouse IFN-γ | W18272D | ICFC |
PerCP/Cyanine5.5 anti-mouse IFN-γ | W18272D | ICFC |
FITC anti-mouse IFN-γ | W18272D | ICFC |
APC anti-mouse IFN-γ | W18272D | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-mouse IFN-γ Antibody
C57BL/6 mouse splenocytes were treated with (left) or withou... -
APC/Fire™ 750 anti-mouse IFN-γ
C57BL/6 mouse splenocytes were treated with (left) or withou... -
PE/Cyanine7 anti-mouse IFN-γ
C57BL/6 mouse splenocytes were treated with (left) or withou... -
PerCP/Cyanine5.5 anti-mouse IFN-γ
C57BL/6 mouse splenocytes were treated with (left) or withou... -
FITC anti-mouse IFN-γ
C57BL/6 mouse splenocytes were treated with (left) or withou... -
APC anti-mouse IFN-γ
C57BL/6 mouse splenocytes were treated with (left) or withou...
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