- Clone
- W19087A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Anterior Gradient 2, Protein Disulphide Isomerase Family Member, HAG-2, PDIA17, XAG-2, HPC8, Epididymis Secretory Protein Li 116, Secreted Cement Gland Homolog, GOB-4, HEL-S-116
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W19087A_PURE_AGR2_Antibody_1_111320 W19087A_PURE_AGR2_Antibody_1_111320](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W19087A_PURE_AGR2_Antibody_1_111320.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg total protein) from HeLa (negative control), A549 (low expressor positive control), and MCF7 (high expressor positive control) lysates were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 2.0 µg/mL (1:250 dilution) purified anti-AGR2 antibody (clone W19087A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. Predicted AGR2 expression was obtained from Human Protein Atlas. -
HeLa cells (negative control, panel A) and A549 cells (positive control, panel B) were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 2.0 µg/mL purified anti-AGR2 antibody (clone W19087A) overnight at 4°C followed by incubation with Alexa Fluor® 594 goat anti-rat IgG antibody (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective. -
Whole cell extracts (250 µg total protein) prepared from MCF7 cells were immunoprecipitated overnight with 2.5 µg of purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400501) or purified anti-AGR2 antibody (clone W19087A). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a rabbit control antibody against a separate epitope of AGR2. Lane M: Molecular weight marker.
Cat # | Size | Price | Save |
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943101 | 25 µg | ¥25,960 | |
943102 | 100 µg | ¥64,460 |
AGR2 is a disulfide isomerase that catalyzes protein folding and thiol-disulfide exchange reactions in the ER lumen. It can also serve as a chaperone for secretory proteins, and is essential for maturation of cysteine-rich transmembrane receptors, including the intestinal glycoprotein mucin. In addition to its role in ER homeostasis, AGR2 can also exert pro-oncogenic function. In many tumors, AGR2 is overexpressed and secreted into the tumor microenvironment, where it disrupts epithelial cell to cell adhesion, promotes invasive structures, and mediates loss of cell polarity. AGR2 is highly upregulated in tamoxifen-treated cells and plays a crucial role in mediating resistance to chemotherapeutic agents.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant human AGR2 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IP - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 2.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone was tested for ICC on A549 cells fixed with 4% PFA and permeabilized with either Triton X-100 or methanol. While both methods were compatible with AGR2 staining, a significantly stronger signal was attained with Triton X-100 permeabilization.
AGR2 staining was highly variable in A549 cells, with many cells lacking any detectable signal. This result was consistent with internal data generated with an anti-AGR2 control antibody. - RRID
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AB_2888903 (BioLegend Cat. No. 943101)
AB_2888903 (BioLegend Cat. No. 943102)
Antigen Details
- Structure
- AGR2 is a 175 amino acid protein with a predicted molecular weight of 20 kD.
- Distribution
-
Primarily expressed in epithelial tissues/Endoplasmic reticulum
- Function
- ER chaperone, protein folding, disulfide isomerase
- Biology Area
- Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Structure, Protein Misfolding and Aggregation, Protein Synthesis
- Antigen References
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1. Chevet E, et al. 2013. Oncogene. 32:2499-509.
2. Fessart D, et al. 2016. Elife. 5:13887.
3. Hengel SM, et al. 2011. J Proteome Res. 10:4567-78. - Gene ID
- 10551 View all products for this Gene ID
- UniProt
- View information about AGR2 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All AGR2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-AGR2 | W19087A | WB,ICC,IP |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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