- Clone
- 1D4-C5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Vitamin B7, Vitamin H
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1D4-C5_Purified_Biotin_Cytof_060304.jpg 1D4-C5_Purified_Biotin_Cytof_060304.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/1D4-C5_Purified_Biotin_Cytof_060304.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human PBMCs stained with Biotin-anti Human CD3 (UCHT1) then detected with 143Nd-anti-Biotin (1D4-C5). Plotted against 147Sm-anti-CD20 (2H7). Data provided by DVS Sciences.
Biotin (vitamin B7 or vitamin H) is essential for cell growth and the metabolization of fat and protein. It is also often used to tag a molecule of interest. The anti-biotin antibody can be used as a second-step reagent for detecting biotinylated targets.
Product DetailsProduct Details
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Biotinylated limulus hemocyanin
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
CyTOF® - Verified - Recommended Usage
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This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
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Additional reported applications (for relevant formats) include: immunoprecipitation and ELISA.
- Additional Product Notes
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Maxpar® is a registered trademark of Standard BioTools Inc.
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Application References
(PubMed link indicates BioLegend citation) -
- Uccellini MB, et al. 2008. J. Immunol. 181:5875.
- Green NM, et al. 2012. J Biol Chem. 287:39789. PubMed.
- Product Citations
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- RRID
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AB_2563707 (BioLegend Cat. No. 409007)
Antigen Details
- Ligand/Receptor
- Avidin, Streptavidin
- Gene ID
- NA
- UniProt
- View information about Biotin on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
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We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
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We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
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We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
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The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All Biotin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Biotin | 1D4-C5 | FC,IP,ELISA |
PE anti-Biotin | 1D4-C5 | FC |
Purified anti-Biotin (Maxpar® Ready) | 1D4-C5 | FC,CyTOF® |
TotalSeq™-A0436 anti-Biotin | 1D4-C5 | PG |
Ultra-LEAF™ Purified anti-Biotin | 1D4-C5 | FC,IP,ELISA |
TotalSeq™-C0436 anti-Biotin | 1D4-C5 | PG |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Biotin
C57BL/6 thymocytes stained with biotinylated CD4 (GK1.5), fo... -
PE anti-Biotin
C57BL/6 thymocytes stained with biotinylated CD4 (GK1.5), fo... -
Purified anti-Biotin (Maxpar® Ready)
Human PBMCs stained with Biotin-anti Human CD3 (UCHT1) then ... -
TotalSeq™-A0436 anti-Biotin
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Ultra-LEAF™ Purified anti-Biotin
C57BL/6 thymocytes stained with biotinylated CD4 (GK1.5), fo... -
TotalSeq™-C0436 anti-Biotin
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