- Clone
- D6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Prolactin-inducible protein (PIP), secretory actin-binding protein, gross cystic disease fluid protein 15, BRST2, GCDFP15, GCDFP-15, GPIP4
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![D6_PURE_BRST-2_Antibody_IHC-P_022819 D6_PURE_BRST-2_Antibody_IHC-P_022819](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/D6_PURE_BRST-2_Antibody_IHC-P_022819.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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IHC staining of anti-BRST-2 (GCDFP-15) antibody (clone D6) on formalin-fixed paraffin-embedded human breast tumor tissue. Following antigen retrieval using Sodium Citrate, the tissue was incubated with the primary antibody at 0.2 µg/ml for 60 minutes at room temperature. BioLegend's Ultra Streptavidin (USA) HRP Kit (Multi-Species) was used for detection followed by counterstaining with hematoxylin.
The BRST-2 antigen is expressed by apocrine sweat glands, eccrine glands (variable), minor salivary glands, bronchial glands, metaplasmic epithelium of the breast, benign sweat gland tumors of the skin and the serous cells of the submandibular gland. Breast carcinomas (primary and metastatic lesions) with apocrine features express the BRST-2 antigen.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was generated against Gross Cystic Disease Fluid Protein 15.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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IHC-P - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.1 - 1.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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The BRST-2 clone D6 recognizes a 15 kD glycoprotein known as Gross Cystic Disease Fluid Protein-15 (GCDFP-15). Antigen retrieval is not required. The BRST-2 epitope is heat-sensitive; paraffin sections that have been heat fixed at 60°C may lose antibody binding sites.
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Application References
(PubMed link indicates BioLegend citation) -
- Yoshimura Y, et al. 2011. Jpn. J. Clin. Oncol. 41:358. (IHC) PubMed
- Fan CY, et al. 2000. Am. J. Surg. Pathol. 24(4):579. PubMed
- Wick MR, et al. 1989. Hum. Pathol. 20(3):281. PubMed
- Mazoujian G, et al. 1984. Am. J. Surg. Pathol. 8(1):43. PubMed
- Mazoujian G, et al. 1983. Am. J. Pathol. 110:105. PubMed
- Brown RW, et al. 1997. Am. J. Clin. Path. 107:12. PubMed
- RRID
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AB_2616964 (BioLegend Cat. No. 915106)
Antigen Details
- Structure
- BRST-2 (GCDFP-15) is a 17 kD protein.
- Distribution
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Extracellular and nucleus.
- Function
- Diseases associated with BRST-2 include florid papillomatosis of the nipple and breast signet ring cell adenocarcinoma. Interacts with AZGP1.
- Biology Area
- Cancer Biomarkers, Cell Biology
- Gene ID
- 5304 View all products for this Gene ID
- UniProt
- View information about BRST-2 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All BRST-2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
BRST-2 (GCDFP-15) Monoclonal Antibody, Purified | D6 | IHC |
Purified anti-BRST-2 (GCDFP-15) | D6 | IHC-P |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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BRST-2 (GCDFP-15) Monoclonal Antibody, Purified
Staining of BRST-2 (GCDFP-15, clone D6) on formalin fixed pa... -
Purified anti-BRST-2 (GCDFP-15)
IHC staining of anti-BRST-2 (GCDFP-15) antibody (clone D6) o...
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