- Clone
- W17190B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CHIP, STIP1 Homology And U-Box Containing Protein 1, Carboxy Terminus Of Hsp70-Interacting Protein, RING-Type E3 Ubiquitin Transferase CHIP, E3 Ubiquitin-Protein Ligase CHIP, CLL-Associated Antigen KW-8, Antigen NY-CO-7, Heat Shock Protein A Binding
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W17190B_Purified_CHIP_Antibody_1_050919 W17190B_Purified_CHIP_Antibody_1_050919](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W17190B_Purified_CHIP_Antibody_1_050919.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-CHIP antibody (W17190B). Lane 1, 5: Molecular weight marker; Lane 2, 6: 20 µg of human spleen lysate; Lane 3, 7: 10 µg of mouse kidney lysate; Lane 4, 8: 10 µg of rat spleen lysate. The blot was incubated with 0.5 µg/mL of the primary antibody or Rat IgG2b isotype control overnight at 4°C, followed by incubation with HRP-labeled Goat anti-rat IgG (Cat. No. 405405). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-CHIP antibody (W17190B) on formalin-fixed paraffin-embedded human spleen tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Biotin-labeled Donkey anti-rat IgG. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm -
IHC staining of purified anti-CHIP antibody (W17190B) on formalin-fixed paraffin-embedded mouse kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Biotin-labeled Donkey anti-rat IgG. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm -
IHC staining of purified anti-CHIP antibody (W17190B) on formalin-fixed paraffin-embedded rat kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Biotin-labeled Donkey anti-rat IgG. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # | Size | Price | Save |
---|---|---|---|
865701 | 25 µg | ¥23,320 | |
865702 | 100 µg | ¥60,720 |
CHIP is an E3 ubiquitin-protein ligase known to ubiquitinate misfolded chaperone-bound substrates and target them for proteasomal degradation. Hsp70, Hsc70, and Hsp90 are three chaperone proteins with which CHIP interacts. CHIP mediates polyubiquitination of DNA polymerase beta at 'Lys-41', 'Lys-61' and 'Lys-81', thereby plays a role in base-excision repair. CHIP can also negatively regulate the suppressive function of regulatory T-cells during inflammation by mediating the ubiquitination and degradation of FOXP3 in a HSPA1A/B-dependent manner. CHIP also negatively regulates TGF-beta signaling by modulating the basal levels of SMAD3 via ubiquitin-mediated degradation. Mutations in CHIP cause spinocerebellar ataxia type 16.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2810779 (BioLegend Cat. No. 865701)
AB_2810780 (BioLegend Cat. No. 865702)
Antigen Details
- Structure
- CHIP is a 303 amino acid protein with an apparent molecular mass of ~ 35 kD.
- Distribution
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Tissue Distribution: Brain, heart, skeletal muscle, kidney, liver, and spleen
Cellular Distribution: Cytosol, nucleus, and endoplasmic reticulum - Function
- CHIP is involved in protein ubiquitination, protein degradation, and neurodegeneration.
- Interaction
- Ubiquitin, ATXN3, EPHA2, SMAD3, UBE4B, VCP/p97.
- Cell Type
- Glutamatergic Neurons, Mature Neurons, Neurons, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neurodegeneration, Neuroscience, Signal Transduction, Ubiquitin/Protein Degradation
- Molecular Family
- Enzymes and Regulators, Heat Shock Proteins, Postsynaptic proteins, Phospho-Proteins, Protein Modifiers
- Antigen References
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- Zhan S, et al. 2017. Int Rev Immunol. 36(5):300.
- Cao Z, et al. 2016. Oncotarget. 7(20):29864.
- Gene ID
- 10273 View all products for this Gene ID 56424 View all products for this Gene ID 287155 View all products for this Gene ID
- UniProt
- View information about CHIP on UniProt.org
Other Formats
View All CHIP Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-CHIP | W17190B | WB,IHC-P |
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